P37677 (LYXK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-xylulose/3-keto-L-gulonate kinase
Short name=L-xylulokinase
EC=2.7.1.-
EC=2.7.1.53

Alternative name(s):
3-dehydro-L-gulonate kinase

Gene names

Name:	lyx
Synonyms:	lyxK, sgbK, xylK, yiaP
Ordered Locus Names:	b3580, JW3552

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorylation of L-xylulose and 3-keto-L-gulonate. Is involved in L-lyxose utilization via xylulose, and may also be involved in the utilization of 2,3-diketo-L-gulonate. Ref.4 Ref.6
Catalytic activity	ATP + L-xylulose = ADP + L-xylulose 5-phosphate. ATP + 3-dehydro-L-gulonate = ADP + 3-dehydro-L-gulonate 6-phosphate.
Subunit structure	Homodimer. Ref.4
Sequence similarities	Belongs to the FGGY kinase family.
Biophysicochemical properties	Kinetic parameters: KM=0.8 mM for L-xylulose Ref.4 KM=0.4 mM for ATP Vmax=33 µmol/min/mg enzyme with L-xylulose as substrate pH dependence: Optimum pH is 8.4 with L-xylulose as substrate.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-lyxose metabolic process Inferred from mutant phenotype Ref.4. Source: EcoCyc
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW L-xylulokinase activity Inferred from direct assay Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	L-xylulose/3-keto-L-gulonate kinase		PRO_0000059560

Sequences

	Sequence		Length	Mass (Da)
	P37677 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 207EC94507141E34	FASTA	498	55,155
	10 20 30 40 50 60 MTQYWLGLDC GGSWLKAGLY DREGREAGVQ RLPLCALSPQ PGWAERDMAE LWQCCMAVIR 70 80 90 100 110 120 ALLTHSGVSG EQIVGIGISA QGKGLFLLDK NDKPLGNAIL SSDRRAMEIV RRWQEDGIPE 130 140 150 160 170 180 KLYPLTRQTL WTGHPVSLLR WLKEHEPERY AQIGCVMMTH DYLRWCLTGV KGCEESNISE 190 200 210 220 230 240 SNLYNMSLGE YDPCLTDWLG IAEINHALPP VVGSAEICGE ITAQTAALTG LKAGTPVVGG 250 260 270 280 290 300 LFDVVSTALC AGIEDEFTLN AVMGTWAVTS GITRGLRDGE AHPYVYGRYV NDGEFIVHEA 310 320 330 340 350 360 SPTSSGNLEW FTAQWGEISF DEINQAVASL PKAGGDLFFL PFLYGSNAGL EMTSGFYGMQ 370 380 390 400 410 420 AIHTRAHLLQ AIYEGVVFSH MTHLNRMRER FTDVHTLRVT GGPAHSDVWM QMLADVSGLR 430 440 450 460 470 480 IELPQVEETG CFGAALAARV GTGVYHNFSE AQRDLRHPVR TLLPDMTAHQ LYQKKYQRYQ 490 HLIAALQGFH ARIKEHTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Activation of a cryptic gene encoding a kinase for L-xylulose opens a new pathway for the utilization of L-lyxose by Escherichia coli." Sanchez J.C., Gimenez R., Schneider A., Fessner W.-D., Baldoma L., Aquilar J., Badia J. J. Biol. Chem. 269:29665-29669(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES. Strain: K12.
[5]	"Novel phosphotransferases system genes revealed by bacterial genome analysis: operons encoding homologues of sugar-specific permease domains of the phosphotransferase system and pentose catabolic enzymes." Reizer J., Charbit A., Reizer A., Saier M.H. Jr. Genome Sci. Technol. 1:53-75(1996) Cited for: DISCUSSION OF SEQUENCE.
[6]	"Utilization of L-ascorbate by Escherichia coli K-12: assignments of functions to products of the yjf-sga and yia-sgb operons." Yew W.S., Gerlt J.A. J. Bacteriol. 184:302-306(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00039 Genomic DNA. Translation: AAB18557.1. U00096 Genomic DNA. Translation: AAC76604.1. AP009048 Genomic DNA. Translation: BAE77713.1.
PIR	S47801.
RefSeq	NP_418037.1. NC_000913.2. YP_491854.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P37677.
SMR	P37677. Positions 6-492.
ModBase	Search...
Protein-protein interaction databases
IntAct	P37677. 2 interactions.
STRING	511145.b3580.
Proteomic databases
PRIDE	P37677.
Protocols and materials databases
DNASU	948101.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76604; AAC76604; b3580. BAE77713; BAE77713; BAE77713.
GeneID	12933556. 948101.
KEGG	ecj:Y75_p3595. eco:b3580.
PATRIC	32122636. VBIEscCol129921_3695.
Organism-specific databases
EchoBASE	EB2192.
EcoGene	EG12284. lyx.
Phylogenomic databases
eggNOG	COG1070.
HOGENOM	HOG000222135.
KO	K00880.
OMA	YGMQALH.
ProtClustDB	CLSK870414.
Enzyme and pathway databases
BioCyc	EcoCyc:LYXK-MONOMER. ECOL316407:JW3552-MONOMER. MetaCyc:LYXK-MONOMER.
Gene expression databases
Genevestigator	P37677.
Family and domain databases
InterPro	IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. [Graphical view]
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYXK_ECOLI

Accession

Primary (citable) accession number: P37677
Secondary accession number(s): Q2M7P3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program