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P37677

- LYXK_ECOLI

UniProt

P37677 - LYXK_ECOLI

Protein

L-xylulose/3-keto-L-gulonate kinase

Gene

lyx

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of L-xylulose and 3-keto-L-gulonate. Is involved in L-lyxose utilization via xylulose, and may also be involved in the utilization of 2,3-diketo-L-gulonate.2 Publications

    Catalytic activityi

    ATP + L-xylulose = ADP + L-xylulose 5-phosphate.
    ATP + 3-dehydro-L-gulonate = ADP + 3-dehydro-L-gulonate 6-phosphate.

    Kineticsi

    1. KM=0.8 mM for L-xylulose1 Publication
    2. KM=0.4 mM for ATP1 Publication

    Vmax=33 µmol/min/mg enzyme with L-xylulose as substrate1 Publication

    pH dependencei

    Optimum pH is 8.4 with L-xylulose as substrate.1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. L-xylulokinase activity Source: EcoCyc

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. L-lyxose metabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:LYXK-MONOMER.
    ECOL316407:JW3552-MONOMER.
    MetaCyc:LYXK-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-xylulose/3-keto-L-gulonate kinase (EC:2.7.1.-, EC:2.7.1.53)
    Short name:
    L-xylulokinase
    Alternative name(s):
    3-dehydro-L-gulonate kinase
    Gene namesi
    Name:lyx
    Synonyms:lyxK, sgbK, xylK, yiaP
    Ordered Locus Names:b3580, JW3552
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12284. lyx.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 498498L-xylulose/3-keto-L-gulonate kinasePRO_0000059560Add
    BLAST

    Proteomic databases

    PRIDEiP37677.

    Expressioni

    Gene expression databases

    GenevestigatoriP37677.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiP37677. 2 interactions.
    STRINGi511145.b3580.

    Structurei

    3D structure databases

    ProteinModelPortaliP37677.
    SMRiP37677. Positions 6-492.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FGGY kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG1070.
    HOGENOMiHOG000222135.
    KOiK00880.
    OMAiQVCAEVV.
    OrthoDBiEOG6KDKMT.
    PhylomeDBiP37677.

    Family and domain databases

    InterProiIPR018485. Carb_kinase_FGGY_C.
    IPR018483. Carb_kinase_FGGY_CS.
    IPR018484. Carb_kinase_FGGY_N.
    [Graphical view]
    PfamiPF02782. FGGY_C. 1 hit.
    PF00370. FGGY_N. 1 hit.
    [Graphical view]
    PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P37677-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQYWLGLDC GGSWLKAGLY DREGREAGVQ RLPLCALSPQ PGWAERDMAE    50
    LWQCCMAVIR ALLTHSGVSG EQIVGIGISA QGKGLFLLDK NDKPLGNAIL 100
    SSDRRAMEIV RRWQEDGIPE KLYPLTRQTL WTGHPVSLLR WLKEHEPERY 150
    AQIGCVMMTH DYLRWCLTGV KGCEESNISE SNLYNMSLGE YDPCLTDWLG 200
    IAEINHALPP VVGSAEICGE ITAQTAALTG LKAGTPVVGG LFDVVSTALC 250
    AGIEDEFTLN AVMGTWAVTS GITRGLRDGE AHPYVYGRYV NDGEFIVHEA 300
    SPTSSGNLEW FTAQWGEISF DEINQAVASL PKAGGDLFFL PFLYGSNAGL 350
    EMTSGFYGMQ AIHTRAHLLQ AIYEGVVFSH MTHLNRMRER FTDVHTLRVT 400
    GGPAHSDVWM QMLADVSGLR IELPQVEETG CFGAALAARV GTGVYHNFSE 450
    AQRDLRHPVR TLLPDMTAHQ LYQKKYQRYQ HLIAALQGFH ARIKEHTL 498
    Length:498
    Mass (Da):55,155
    Last modified:October 1, 1994 - v1
    Checksum:i207EC94507141E34
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00039 Genomic DNA. Translation: AAB18557.1.
    U00096 Genomic DNA. Translation: AAC76604.1.
    AP009048 Genomic DNA. Translation: BAE77713.1.
    PIRiS47801.
    RefSeqiNP_418037.1. NC_000913.3.
    YP_491854.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76604; AAC76604; b3580.
    BAE77713; BAE77713; BAE77713.
    GeneIDi12933556.
    948101.
    KEGGiecj:Y75_p3595.
    eco:b3580.
    PATRICi32122636. VBIEscCol129921_3695.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00039 Genomic DNA. Translation: AAB18557.1 .
    U00096 Genomic DNA. Translation: AAC76604.1 .
    AP009048 Genomic DNA. Translation: BAE77713.1 .
    PIRi S47801.
    RefSeqi NP_418037.1. NC_000913.3.
    YP_491854.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P37677.
    SMRi P37677. Positions 6-492.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P37677. 2 interactions.
    STRINGi 511145.b3580.

    Proteomic databases

    PRIDEi P37677.

    Protocols and materials databases

    DNASUi 948101.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76604 ; AAC76604 ; b3580 .
    BAE77713 ; BAE77713 ; BAE77713 .
    GeneIDi 12933556.
    948101.
    KEGGi ecj:Y75_p3595.
    eco:b3580.
    PATRICi 32122636. VBIEscCol129921_3695.

    Organism-specific databases

    EchoBASEi EB2192.
    EcoGenei EG12284. lyx.

    Phylogenomic databases

    eggNOGi COG1070.
    HOGENOMi HOG000222135.
    KOi K00880.
    OMAi QVCAEVV.
    OrthoDBi EOG6KDKMT.
    PhylomeDBi P37677.

    Enzyme and pathway databases

    BioCyci EcoCyc:LYXK-MONOMER.
    ECOL316407:JW3552-MONOMER.
    MetaCyc:LYXK-MONOMER.

    Miscellaneous databases

    PROi P37677.

    Gene expression databases

    Genevestigatori P37677.

    Family and domain databases

    InterProi IPR018485. Carb_kinase_FGGY_C.
    IPR018483. Carb_kinase_FGGY_CS.
    IPR018484. Carb_kinase_FGGY_N.
    [Graphical view ]
    Pfami PF02782. FGGY_C. 1 hit.
    PF00370. FGGY_N. 1 hit.
    [Graphical view ]
    PROSITEi PS00445. FGGY_KINASES_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
      Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
      Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Activation of a cryptic gene encoding a kinase for L-xylulose opens a new pathway for the utilization of L-lyxose by Escherichia coli."
      Sanchez J.C., Gimenez R., Schneider A., Fessner W.-D., Baldoma L., Aquilar J., Badia J.
      J. Biol. Chem. 269:29665-29669(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: K12.
    5. "Novel phosphotransferases system genes revealed by bacterial genome analysis: operons encoding homologues of sugar-specific permease domains of the phosphotransferase system and pentose catabolic enzymes."
      Reizer J., Charbit A., Reizer A., Saier M.H. Jr.
      Genome Sci. Technol. 1:53-75(1996)
      Cited for: DISCUSSION OF SEQUENCE.
    6. "Utilization of L-ascorbate by Escherichia coli K-12: assignments of functions to products of the yjf-sga and yia-sgb operons."
      Yew W.S., Gerlt J.A.
      J. Bacteriol. 184:302-306(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiLYXK_ECOLI
    AccessioniPrimary (citable) accession number: P37677
    Secondary accession number(s): Q2M7P3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3