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P37677 (LYXK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-xylulose/3-keto-L-gulonate kinase

Short name=L-xylulokinase
EC=2.7.1.-
EC=2.7.1.53
Alternative name(s):
3-dehydro-L-gulonate kinase
Gene names
Name:lyx
Synonyms:lyxK, sgbK, xylK, yiaP
Ordered Locus Names:b3580, JW3552
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of L-xylulose and 3-keto-L-gulonate. Is involved in L-lyxose utilization via xylulose, and may also be involved in the utilization of 2,3-diketo-L-gulonate. Ref.4 Ref.6

Catalytic activity

ATP + L-xylulose = ADP + L-xylulose 5-phosphate.

ATP + 3-dehydro-L-gulonate = ADP + 3-dehydro-L-gulonate 6-phosphate.

Subunit structure

Homodimer. Ref.4

Sequence similarities

Belongs to the FGGY kinase family.

Biophysicochemical properties

Kinetic parameters:

KM=0.8 mM for L-xylulose Ref.4

KM=0.4 mM for ATP

Vmax=33 µmol/min/mg enzyme with L-xylulose as substrate

pH dependence:

Optimum pH is 8.4 with L-xylulose as substrate.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498L-xylulose/3-keto-L-gulonate kinase
PRO_0000059560

Sequences

Sequence LengthMass (Da)Tools
P37677 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 207EC94507141E34

FASTA49855,155
        10         20         30         40         50         60 
MTQYWLGLDC GGSWLKAGLY DREGREAGVQ RLPLCALSPQ PGWAERDMAE LWQCCMAVIR 

        70         80         90        100        110        120 
ALLTHSGVSG EQIVGIGISA QGKGLFLLDK NDKPLGNAIL SSDRRAMEIV RRWQEDGIPE 

       130        140        150        160        170        180 
KLYPLTRQTL WTGHPVSLLR WLKEHEPERY AQIGCVMMTH DYLRWCLTGV KGCEESNISE 

       190        200        210        220        230        240 
SNLYNMSLGE YDPCLTDWLG IAEINHALPP VVGSAEICGE ITAQTAALTG LKAGTPVVGG 

       250        260        270        280        290        300 
LFDVVSTALC AGIEDEFTLN AVMGTWAVTS GITRGLRDGE AHPYVYGRYV NDGEFIVHEA 

       310        320        330        340        350        360 
SPTSSGNLEW FTAQWGEISF DEINQAVASL PKAGGDLFFL PFLYGSNAGL EMTSGFYGMQ 

       370        380        390        400        410        420 
AIHTRAHLLQ AIYEGVVFSH MTHLNRMRER FTDVHTLRVT GGPAHSDVWM QMLADVSGLR 

       430        440        450        460        470        480 
IELPQVEETG CFGAALAARV GTGVYHNFSE AQRDLRHPVR TLLPDMTAHQ LYQKKYQRYQ 

       490 
HLIAALQGFH ARIKEHTL 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Activation of a cryptic gene encoding a kinase for L-xylulose opens a new pathway for the utilization of L-lyxose by Escherichia coli."
Sanchez J.C., Gimenez R., Schneider A., Fessner W.-D., Baldoma L., Aquilar J., Badia J.
J. Biol. Chem. 269:29665-29669(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: K12.
[5]"Novel phosphotransferases system genes revealed by bacterial genome analysis: operons encoding homologues of sugar-specific permease domains of the phosphotransferase system and pentose catabolic enzymes."
Reizer J., Charbit A., Reizer A., Saier M.H. Jr.
Genome Sci. Technol. 1:53-75(1996)
Cited for: DISCUSSION OF SEQUENCE.
[6]"Utilization of L-ascorbate by Escherichia coli K-12: assignments of functions to products of the yjf-sga and yia-sgb operons."
Yew W.S., Gerlt J.A.
J. Bacteriol. 184:302-306(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00039 Genomic DNA. Translation: AAB18557.1.
U00096 Genomic DNA. Translation: AAC76604.1.
AP009048 Genomic DNA. Translation: BAE77713.1.
PIRS47801.
RefSeqNP_418037.1. NC_000913.3.
YP_491854.1. NC_007779.1.

3D structure databases

ProteinModelPortalP37677.
SMRP37677. Positions 6-492.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP37677. 2 interactions.
STRING511145.b3580.

Proteomic databases

PRIDEP37677.

Protocols and materials databases

DNASU948101.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76604; AAC76604; b3580.
BAE77713; BAE77713; BAE77713.
GeneID12933556.
948101.
KEGGecj:Y75_p3595.
eco:b3580.
PATRIC32122636. VBIEscCol129921_3695.

Organism-specific databases

EchoBASEEB2192.
EcoGeneEG12284. lyx.

Phylogenomic databases

eggNOGCOG1070.
HOGENOMHOG000222135.
KOK00880.
OMAQVCAEVV.
OrthoDBEOG6KDKMT.
PhylomeDBP37677.

Enzyme and pathway databases

BioCycEcoCyc:LYXK-MONOMER.
ECOL316407:JW3552-MONOMER.
MetaCyc:LYXK-MONOMER.

Gene expression databases

GenevestigatorP37677.

Family and domain databases

InterProIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
[Graphical view]
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PROSITEPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP37677.

Entry information

Entry nameLYXK_ECOLI
AccessionPrimary (citable) accession number: P37677
Secondary accession number(s): Q2M7P3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 14, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene