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Protein

2,3-diketo-L-gulonate-binding periplasmic protein YiaO

Gene

yiaO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the tripartite ATP-independent periplasmic (TRAP) transport system YiaMNO involved in the uptake of 2,3-diketo-L-gulonate. This protein specifically binds 2,3-diketo-L-gulonate. Is not able to bind either L-ascorbate or dehydroascorbate.1 Publication

GO - Molecular functioni

  • carbohydrate binding Source: EcoCyc

GO - Biological processi

  • carbohydrate transmembrane transport Source: EcoCyc
  • cellular response to DNA damage stimulus Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG12283-MONOMER.
ECOL316407:JW3551-MONOMER.
MetaCyc:EG12283-MONOMER.

Protein family/group databases

TCDBi2.A.56.1.2. the tripartite atp-independent periplasmic transporter (trap-t) family.

Names & Taxonomyi

Protein namesi
Recommended name:
2,3-diketo-L-gulonate-binding periplasmic protein YiaO
Short name:
2,3-DKG-binding protein
Alternative name(s):
Extracytoplasmic solute receptor protein YiaO
Gene namesi
Name:yiaO
Ordered Locus Names:b3579, JW3551
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12283. yiaO.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24CuratedAdd BLAST24
ChainiPRO_000003181325 – 3282,3-diketo-L-gulonate-binding periplasmic protein YiaOAdd BLAST304

Proteomic databases

PaxDbiP37676.
PRIDEiP37676.

Interactioni

Subunit structurei

The complex comprises the extracytoplasmic solute receptor protein YiaO, and the two transmembrane proteins YiaM and YiaN.

Protein-protein interaction databases

BioGridi4262009. 5 interactors.
STRINGi511145.b3579.

Structurei

3D structure databases

ProteinModelPortaliP37676.
SMRiP37676.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DR6. Bacteria.
COG1638. LUCA.
HOGENOMiHOG000251307.
InParanoidiP37676.
OMAiGQAADYF.
PhylomeDBiP37676.

Family and domain databases

InterProiIPR004682. TRAP_DctP.
IPR018389. TRAP_DctP/TeaA.
[Graphical view]
PfamiPF03480. DctP. 1 hit.
[Graphical view]
PIRSFiPIRSF006470. DctB. 1 hit.
TIGRFAMsiTIGR00787. dctP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLRSVTYAL FIAGLAAFST SSLAAQSLRF GYETSQTDSQ HIAAKKFNDL
60 70 80 90 100
LQERTKGELK LKLFPDSTLG NAQAMISGVR GGTIDMEMSG SNNFAGLSPV
110 120 130 140 150
MNLLDVPFLF RDTAHAHKTL DGKVGDDLKA SLEGKGLKVL AYWENGWRDV
160 170 180 190 200
TNSRAPVKTP ADLKGLKIRT NNSPMNIAAF KVFGANPIPM PFAEVYTGLE
210 220 230 240 250
TRTIDAQEHP INVVWSAKFF EVQKFLSLTH HAYSPLLVVI NKAKFDGLSP
260 270 280 290 300
EFQQALVSSA QEAGNYQRKL VAEDQQKIID GMKEAGVEVI TDLDRKAFSD
310 320
ALGNQVRDMF VKDVPQGADL LKAVDEVQ
Length:328
Mass (Da):35,970
Last modified:October 1, 1994 - v1
Checksum:iEB44605FE87BCFF8
GO

Mass spectrometryi

Molecular mass is 33468.8±2 Da from positions 25 - 328. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18556.1.
U00096 Genomic DNA. Translation: AAC76603.1.
AP009048 Genomic DNA. Translation: BAE77714.1.
PIRiS47800.
RefSeqiNP_418036.1. NC_000913.3.
WP_000776887.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76603; AAC76603; b3579.
BAE77714; BAE77714; BAE77714.
GeneIDi948091.
KEGGiecj:JW3551.
eco:b3579.
PATRICi32122634. VBIEscCol129921_3694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18556.1.
U00096 Genomic DNA. Translation: AAC76603.1.
AP009048 Genomic DNA. Translation: BAE77714.1.
PIRiS47800.
RefSeqiNP_418036.1. NC_000913.3.
WP_000776887.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37676.
SMRiP37676.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262009. 5 interactors.
STRINGi511145.b3579.

Protein family/group databases

TCDBi2.A.56.1.2. the tripartite atp-independent periplasmic transporter (trap-t) family.

Proteomic databases

PaxDbiP37676.
PRIDEiP37676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76603; AAC76603; b3579.
BAE77714; BAE77714; BAE77714.
GeneIDi948091.
KEGGiecj:JW3551.
eco:b3579.
PATRICi32122634. VBIEscCol129921_3694.

Organism-specific databases

EchoBASEiEB2191.
EcoGeneiEG12283. yiaO.

Phylogenomic databases

eggNOGiENOG4105DR6. Bacteria.
COG1638. LUCA.
HOGENOMiHOG000251307.
InParanoidiP37676.
OMAiGQAADYF.
PhylomeDBiP37676.

Enzyme and pathway databases

BioCyciEcoCyc:EG12283-MONOMER.
ECOL316407:JW3551-MONOMER.
MetaCyc:EG12283-MONOMER.

Miscellaneous databases

PROiP37676.

Family and domain databases

InterProiIPR004682. TRAP_DctP.
IPR018389. TRAP_DctP/TeaA.
[Graphical view]
PfamiPF03480. DctP. 1 hit.
[Graphical view]
PIRSFiPIRSF006470. DctB. 1 hit.
TIGRFAMsiTIGR00787. dctP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYIAO_ECOLI
AccessioniPrimary (citable) accession number: P37676
Secondary accession number(s): Q2M7P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally proposed to be a subunit from an L-xylulose uptake system, but PubMed:16385129 shows that YiaO does not bind L- or D-xylulose.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.