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Protein

2,3-diketo-L-gulonate reductase

Gene

dlgD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH, to form 3-keto-L-gulonate.1 Publication

Miscellaneous

NAD is bound in an unusual conformation, at the interface of a dimer of the enzyme.

Catalytic activityi

3-dehydro-L-gulonate + NAD(P)+ = (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H.

Enzyme regulationi

The enzyme is inhibited by tartrate and D-malate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44Proton donorCurated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 174NAD7
Nucleotide bindingi224 – 225NAD2
Nucleotide bindingi304 – 306NAD3

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:EG12279-MONOMER
MetaCyc:EG12279-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
2,3-diketo-L-gulonate reductase (EC:1.1.1.130)
Short name:
2,3-DKG reductase
Alternative name(s):
3-dehydro-L-gulonate 2-dehydrogenase
Gene namesi
Name:dlgD
Synonyms:yiaK
Ordered Locus Names:b3575, JW3547
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12279 dlgD

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB01694 D-tartaric acid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000838301 – 3322,3-diketo-L-gulonate reductaseAdd BLAST332

Proteomic databases

PaxDbiP37672
PRIDEiP37672

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4262546, 12 interactors
STRINGi316385.ECDH10B_3756

Structurei

Secondary structure

1332
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 18Combined sources14
Helixi23 – 38Combined sources16
Turni43 – 45Combined sources3
Helixi46 – 48Combined sources3
Helixi49 – 57Combined sources9
Beta strandi68 – 73Combined sources6
Beta strandi76 – 80Combined sources5
Helixi86 – 104Combined sources19
Beta strandi105 – 114Combined sources10
Helixi121 – 129Combined sources9
Beta strandi133 – 138Combined sources6
Beta strandi149 – 151Combined sources3
Beta strandi159 – 163Combined sources5
Beta strandi169 – 173Combined sources5
Beta strandi175 – 178Combined sources4
Helixi180 – 188Combined sources9
Beta strandi203 – 206Combined sources4
Helixi209 – 215Combined sources7
Turni221 – 223Combined sources3
Helixi224 – 241Combined sources18
Helixi246 – 252Combined sources7
Beta strandi254 – 256Combined sources3
Beta strandi258 – 266Combined sources9
Turni269 – 271Combined sources3
Helixi274 – 289Combined sources16
Beta strandi293 – 295Combined sources3
Turni303 – 305Combined sources3
Helixi306 – 317Combined sources12
Helixi323 – 331Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NXUX-ray1.80A/B1-332[»]
1S20X-ray2.20A/B/C/D/E/F/G/H1-332[»]
ProteinModelPortaliP37672
SMRiP37672
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37672

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DG4 Bacteria
COG2055 LUCA
HOGENOMiHOG000249660
KOiK08092
OMAiDGHYVLG
PhylomeDBiP37672

Family and domain databases

HAMAPiMF_00820 Diketo_gul_reduc, 1 hit
InterProiView protein in InterPro
IPR023689 Diketo_gul_Rdtase
IPR036111 Mal/L-sulfo/L-lacto_DH-like_sf
IPR003767 Malate/L-lactate_DH-like
PANTHERiPTHR11091 PTHR11091, 1 hit
PfamiView protein in Pfam
PF02615 Ldh_2, 1 hit
SUPFAMiSSF89733 SSF89733, 1 hit

Sequencei

Sequence statusi: Complete.

P37672-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVTFEQLKA AFNRVLISRG VDSETADACA EMFARTTESG VYSHGVNRFP
60 70 80 90 100
RFIQQLENGD IIPDAQPKRI TSLGAIEQWD AQRSIGNLTA KKMMDRAIEL
110 120 130 140 150
AADHGIGLVA LRNANHWMRG GSYGWQAAEK GYIGICWTNS IAVMPPWGAK
160 170 180 190 200
ECRIGTNPLI VAIPSTPITM VDMSMSMFSY GMLEVNRLAG RQLPVDGGFD
210 220 230 240 250
DEGNLTKEPG VIEKNRRILP MGYWKGSGMS IVLDMIATLL SDGASVAEVT
260 270 280 290 300
QDNSDEYGIS QIFIAIEVDK LIDGPTRDAK LQRIMDYVTS AERADENQAI
310 320 330
RLPGHEFTTL LAENRRNGIT VDDSVWAKIQ AL
Length:332
Mass (Da):36,573
Last modified:November 1, 1997 - v2
Checksum:iA411EB14D5C03DF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA Translation: AAB18552.1
U00096 Genomic DNA Translation: AAC76599.1
AP009048 Genomic DNA Translation: BAE77718.1
AJ223475 Genomic DNA Translation: CAA11398.1
PIRiA65157
RefSeqiNP_418032.1, NC_000913.3
WP_000869037.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76599; AAC76599; b3575
BAE77718; BAE77718; BAE77718
GeneIDi948096
KEGGiecj:JW3547
eco:b3575
PATRICifig|1411691.4.peg.3137

Entry informationi

Entry nameiDLGD_ECOLI
AccessioniPrimary (citable) accession number: P37672
Secondary accession number(s): P76716, Q2M7N8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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