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Protein

HTH-type transcriptional regulator YiaJ

Gene

yiaJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi45 – 6420H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • transcription factor activity, sequence-specific DNA binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12278-MONOMER.
ECOL316407:JW3546-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator YiaJ
Alternative name(s):
YiaKLMNOPQRS operon repressor
Short name:
YiaK-S operon repressor
Gene namesi
Name:yiaJ
Ordered Locus Names:b3574, JW3546
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12278. yiaJ.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282HTH-type transcriptional regulator YiaJPRO_0000201771Add
BLAST

Proteomic databases

PaxDbiP37671.
PRIDEiP37671.

Expressioni

Inductioni

Expression of yiaJ is autogenously regulated and reduced by the binding of CRP-cAMP to the CRP site 1 of the YiaK-S promoter.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259370. 4 interactions.
IntActiP37671. 3 interactions.
MINTiMINT-1308441.
STRINGi511145.b3574.

Structurei

Secondary structure

1
282
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi98 – 11518Combined sources
Beta strandi117 – 1259Combined sources
Beta strandi128 – 1358Combined sources
Beta strandi151 – 1533Combined sources
Helixi154 – 1563Combined sources
Helixi158 – 1658Combined sources
Helixi169 – 17810Combined sources
Turni179 – 1824Combined sources
Helixi194 – 20714Combined sources
Beta strandi210 – 2178Combined sources
Beta strandi220 – 2278Combined sources
Beta strandi235 – 2439Combined sources
Helixi244 – 2507Combined sources
Helixi252 – 27221Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YSQX-ray1.75A94-282[»]
ProteinModelPortaliP37671.
SMRiP37671. Positions 24-273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37671.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 8563HTH iclR-typePROSITE-ProRule annotationAdd
BLAST
Domaini100 – 272173IclR-EDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH iclR-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 iclR-ED (iclR effector binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105J4Q. Bacteria.
COG1414. LUCA.
HOGENOMiHOG000107041.
InParanoidiP37671.
OMAiHNKNSIT.
OrthoDBiEOG6MWN8D.
PhylomeDBiP37671.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.450.40. 1 hit.
InterProiIPR029016. GAF_dom-like.
IPR014757. Tscrpt_reg_IclR_C.
IPR005471. Tscrpt_reg_IclR_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF09339. HTH_IclR. 1 hit.
PF01614. IclR. 1 hit.
[Graphical view]
SMARTiSM00346. HTH_ICLR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55781. SSF55781. 1 hit.
PROSITEiPS51077. HTH_ICLR. 1 hit.
PS51078. ICLR_ED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37671-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKEVMGKKE NEMAQEKERP AGSQSLFRGL MLIEILSNYP NGCPLAHLSE
60 70 80 90 100
LAGLNKSTVH RLLQGLQSCG YVTTAPAAGS YRLTTKFIAV GQKALSSLNI
110 120 130 140 150
IHIAAPHLEA LNIATGETIN FSSREDDHAI LIYKLEPTTG MLRTRAYIGQ
160 170 180 190 200
HMPLYCSAMG KIYMAFGHPD YVKSYWESHQ HEIQPLTRNT ITELPAMFDE
210 220 230 240 250
LAHIRESGAA MDREENELGV SCIAVPVFDI HGRVPYAVSI SLSTSRLKQV
260 270 280
GEKNLLKPLR ETAQAISNEL GFTVRDDLGA IT
Length:282
Mass (Da):31,067
Last modified:October 1, 1994 - v1
Checksum:i22E887B6672B958F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18551.1.
U00096 Genomic DNA. Translation: AAC76598.1.
AP009048 Genomic DNA. Translation: BAE77719.1.
PIRiS47795.
RefSeqiNP_418031.1. NC_000913.3.
WP_000514240.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76598; AAC76598; b3574.
BAE77719; BAE77719; BAE77719.
GeneIDi948084.
KEGGiecj:JW3546.
eco:b3574.
PATRICi32122624. VBIEscCol129921_3689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18551.1.
U00096 Genomic DNA. Translation: AAC76598.1.
AP009048 Genomic DNA. Translation: BAE77719.1.
PIRiS47795.
RefSeqiNP_418031.1. NC_000913.3.
WP_000514240.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YSQX-ray1.75A94-282[»]
ProteinModelPortaliP37671.
SMRiP37671. Positions 24-273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259370. 4 interactions.
IntActiP37671. 3 interactions.
MINTiMINT-1308441.
STRINGi511145.b3574.

Proteomic databases

PaxDbiP37671.
PRIDEiP37671.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76598; AAC76598; b3574.
BAE77719; BAE77719; BAE77719.
GeneIDi948084.
KEGGiecj:JW3546.
eco:b3574.
PATRICi32122624. VBIEscCol129921_3689.

Organism-specific databases

EchoBASEiEB2186.
EcoGeneiEG12278. yiaJ.

Phylogenomic databases

eggNOGiENOG4105J4Q. Bacteria.
COG1414. LUCA.
HOGENOMiHOG000107041.
InParanoidiP37671.
OMAiHNKNSIT.
OrthoDBiEOG6MWN8D.
PhylomeDBiP37671.

Enzyme and pathway databases

BioCyciEcoCyc:EG12278-MONOMER.
ECOL316407:JW3546-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37671.
PROiP37671.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.450.40. 1 hit.
InterProiIPR029016. GAF_dom-like.
IPR014757. Tscrpt_reg_IclR_C.
IPR005471. Tscrpt_reg_IclR_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF09339. HTH_IclR. 1 hit.
PF01614. IclR. 1 hit.
[Graphical view]
SMARTiSM00346. HTH_ICLR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55781. SSF55781. 1 hit.
PROSITEiPS51077. HTH_ICLR. 1 hit.
PS51078. ICLR_ED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Regulation of expression of the yiaKLMNOPQRS operon for carbohydrate utilization in Escherichia coli: involvement of the main transcriptional factors."
    Ibanez E., Campos E., Baldoma L., Aguilar J., Badia J.
    J. Bacteriol. 182:4617-4624(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
    Strain: K12.
  5. "The crystal structure of transcriptional regulator YiaJ."
    Midwest center for structural genomics (MCSG)
    Submitted (MAR-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 94-282.

Entry informationi

Entry nameiYIAJ_ECOLI
AccessioniPrimary (citable) accession number: P37671
Secondary accession number(s): Q2M7N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 20, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.