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Protein

DNA-binding transcriptional repressor YiaJ

Gene

yiaJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi45 – 64H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • DNA binding transcription factor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG12278-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding transcriptional repressor YiaJ
Alternative name(s):
YiaKLMNOPQRS operon repressor
Short name:
YiaK-S operon repressor
Gene namesi
Name:yiaJ
Ordered Locus Names:b3574, JW3546
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12278 yiaJ

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002017711 – 282DNA-binding transcriptional repressor YiaJAdd BLAST282

Proteomic databases

PaxDbiP37671
PRIDEiP37671

Expressioni

Inductioni

Expression of yiaJ is autogenously regulated and reduced by the binding of CRP-cAMP to the CRP site 1 of the YiaK-S promoter.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259370, 4 interactors
IntActiP37671, 3 interactors
STRINGi316385.ECDH10B_3755

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi98 – 115Combined sources18
Beta strandi117 – 125Combined sources9
Beta strandi128 – 135Combined sources8
Beta strandi151 – 153Combined sources3
Helixi154 – 156Combined sources3
Helixi158 – 165Combined sources8
Helixi169 – 178Combined sources10
Turni179 – 182Combined sources4
Helixi194 – 207Combined sources14
Beta strandi210 – 217Combined sources8
Beta strandi220 – 227Combined sources8
Beta strandi235 – 243Combined sources9
Helixi244 – 250Combined sources7
Helixi252 – 272Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YSQX-ray1.75A94-282[»]
ProteinModelPortaliP37671
SMRiP37671
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37671

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 85HTH iclR-typePROSITE-ProRule annotationAdd BLAST63
Domaini100 – 272IclR-EDPROSITE-ProRule annotationAdd BLAST173

Phylogenomic databases

eggNOGiENOG4105J4Q Bacteria
COG1414 LUCA
HOGENOMiHOG000107041
InParanoidiP37671
KOiK21602
OMAiWPLRMHL
PhylomeDBiP37671

Family and domain databases

Gene3Di1.10.10.10, 1 hit
3.30.450.40, 1 hit
InterProiView protein in InterPro
IPR029016 GAF-like_dom_sf
IPR014757 Tscrpt_reg_IclR_C
IPR005471 Tscrpt_reg_IclR_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF09339 HTH_IclR, 1 hit
PF01614 IclR, 1 hit
SMARTiView protein in SMART
SM00346 HTH_ICLR, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS51077 HTH_ICLR, 1 hit
PS51078 ICLR_ED, 1 hit

Sequencei

Sequence statusi: Complete.

P37671-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKEVMGKKE NEMAQEKERP AGSQSLFRGL MLIEILSNYP NGCPLAHLSE
60 70 80 90 100
LAGLNKSTVH RLLQGLQSCG YVTTAPAAGS YRLTTKFIAV GQKALSSLNI
110 120 130 140 150
IHIAAPHLEA LNIATGETIN FSSREDDHAI LIYKLEPTTG MLRTRAYIGQ
160 170 180 190 200
HMPLYCSAMG KIYMAFGHPD YVKSYWESHQ HEIQPLTRNT ITELPAMFDE
210 220 230 240 250
LAHIRESGAA MDREENELGV SCIAVPVFDI HGRVPYAVSI SLSTSRLKQV
260 270 280
GEKNLLKPLR ETAQAISNEL GFTVRDDLGA IT
Length:282
Mass (Da):31,067
Last modified:October 1, 1994 - v1
Checksum:i22E887B6672B958F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA Translation: AAB18551.1
U00096 Genomic DNA Translation: AAC76598.1
AP009048 Genomic DNA Translation: BAE77719.1
PIRiS47795
RefSeqiNP_418031.1, NC_000913.3
WP_000514240.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76598; AAC76598; b3574
BAE77719; BAE77719; BAE77719
GeneIDi948084
KEGGiecj:JW3546
eco:b3574
PATRICifig|1411691.4.peg.3138

Similar proteinsi

Entry informationi

Entry nameiYIAJ_ECOLI
AccessioniPrimary (citable) accession number: P37671
Secondary accession number(s): Q2M7N7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: March 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health