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Protein

O-acetyltransferase WecH

Gene

wecH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the incorporation of O-acetyl groups into the enterobacterial common antigen (ECA) trisaccharide repeat units. Catalyzes the acetylation of both cyclic ECA (ECA(CYC)) and phosphoglyceride-linked ECA (ECA(PG)).1 Publication

Pathwayi: enterobacterial common antigen biosynthesis

This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • O-acetyltransferase activity Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG12274-MONOMER.
ECOL316407:JW3533-MONOMER.
BRENDAi3.1.8.1. 2165.
UniPathwayiUPA00566.

Protein family/group databases

TCDBi9.B.97.1.1. the acyltransferase-3/putative acetyl-coa transporter (atat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
O-acetyltransferase WecH1 PublicationUniRule annotation (EC:2.3.1.-UniRule annotation1 Publication)
Gene namesi
Name:wecH1 PublicationUniRule annotation
Synonyms:yiaH
Ordered Locus Names:b3561, JW3533
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12274. wecH.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicCuratedAdd BLAST12
Transmembranei13 – 33HelicalUniRule annotationAdd BLAST21
Topological domaini34 – 45PeriplasmicCuratedAdd BLAST12
Transmembranei46 – 66HelicalUniRule annotationAdd BLAST21
Topological domaini67 – 78CytoplasmicCuratedAdd BLAST12
Transmembranei79 – 99HelicalUniRule annotationAdd BLAST21
Topological domaini100 – 113PeriplasmicCuratedAdd BLAST14
Transmembranei114 – 134HelicalUniRule annotationAdd BLAST21
Topological domaini135 – 138CytoplasmicCurated4
Transmembranei139 – 159HelicalUniRule annotationAdd BLAST21
Topological domaini160 – 168PeriplasmicCurated9
Transmembranei169 – 189HelicalUniRule annotationAdd BLAST21
Topological domaini190 – 202CytoplasmicCuratedAdd BLAST13
Transmembranei203 – 223HelicalUniRule annotationAdd BLAST21
Topological domaini224 – 234PeriplasmicCuratedAdd BLAST11
Transmembranei235 – 255HelicalUniRule annotationAdd BLAST21
Topological domaini256 – 266CytoplasmicCuratedAdd BLAST11
Transmembranei267 – 287HelicalUniRule annotationAdd BLAST21
Topological domaini288 – 299PeriplasmicCuratedAdd BLAST12
Transmembranei300 – 320HelicalUniRule annotationAdd BLAST21
Topological domaini321 – 331Cytoplasmic1 PublicationAdd BLAST11

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB-HAMAP
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant is defective in the incorporation of O-acetyl groups into both ECA(CYC) and ECA(PG).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002080981 – 331O-acetyltransferase WecHAdd BLAST331

Proteomic databases

PaxDbiP37669.
PRIDEiP37669.

Interactioni

Protein-protein interaction databases

DIPiDIP-12403N.
IntActiP37669. 1 interactor.
MINTiMINT-1289754.
STRINGi511145.b3561.

Structurei

3D structure databases

ProteinModelPortaliP37669.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acyltransferase 3 family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105MEV. Bacteria.
COG3274. LUCA.
HOGENOMiHOG000126046.
OMAiFYYVLYA.

Family and domain databases

HAMAPiMF_01949. Acetyltr_WecH. 1 hit.
InterProiIPR002656. Acyl_transf_3.
IPR032905. WecH.
[Graphical view]
PfamiPF01757. Acyl_transf_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37669-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPKIYWIDN LRGIACLMVV MIHTTTWYVT NAHSVSPVTW DIANVLNSAS
60 70 80 90 100
RVSVPLFFMI SGYLFFGERS AQPRHFLRIG LCLIFYSAIA LLYIALFTSI
110 120 130 140 150
NMELALKNLL QKPVFYHLWF FFAIAVIYLV SPLIQVKNVG GKMLLVLMAV
160 170 180 190 200
IGIIANPNTV PQKIDGFEWL PINLYINGDT FYYILYGMLG RAIGMMDTQH
210 220 230 240 250
KALSWVSAAL FATGVFIISR GTLYELQWRG NFADTWYLYC GPMVFICAIA
260 270 280 290 300
LLTLVKNTLD TRTIRGLGLI SRHSLGIYGF HALIIHALRT RGIELKNWPI
310 320 330
LDIIWIFCAT LAASLLLSML VQRIDRNRLV S
Length:331
Mass (Da):37,570
Last modified:October 1, 1994 - v1
Checksum:i88FD5A182110F63C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18538.1.
U00096 Genomic DNA. Translation: AAC76585.1.
AP009048 Genomic DNA. Translation: BAE77732.1.
PIRiS47782.
RefSeqiNP_418018.1. NC_000913.3.
WP_001182650.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76585; AAC76585; b3561.
BAE77732; BAE77732; BAE77732.
GeneIDi948077.
KEGGiecj:JW3533.
eco:b3561.
PATRICi32122598. VBIEscCol129921_3676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18538.1.
U00096 Genomic DNA. Translation: AAC76585.1.
AP009048 Genomic DNA. Translation: BAE77732.1.
PIRiS47782.
RefSeqiNP_418018.1. NC_000913.3.
WP_001182650.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37669.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-12403N.
IntActiP37669. 1 interactor.
MINTiMINT-1289754.
STRINGi511145.b3561.

Protein family/group databases

TCDBi9.B.97.1.1. the acyltransferase-3/putative acetyl-coa transporter (atat) family.

Proteomic databases

PaxDbiP37669.
PRIDEiP37669.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76585; AAC76585; b3561.
BAE77732; BAE77732; BAE77732.
GeneIDi948077.
KEGGiecj:JW3533.
eco:b3561.
PATRICi32122598. VBIEscCol129921_3676.

Organism-specific databases

EchoBASEiEB2183.
EcoGeneiEG12274. wecH.

Phylogenomic databases

eggNOGiENOG4105MEV. Bacteria.
COG3274. LUCA.
HOGENOMiHOG000126046.
OMAiFYYVLYA.

Enzyme and pathway databases

UniPathwayiUPA00566.
BioCyciEcoCyc:EG12274-MONOMER.
ECOL316407:JW3533-MONOMER.
BRENDAi3.1.8.1. 2165.

Miscellaneous databases

PROiP37669.

Family and domain databases

HAMAPiMF_01949. Acetyltr_WecH. 1 hit.
InterProiIPR002656. Acyl_transf_3.
IPR032905. WecH.
[Graphical view]
PfamiPF01757. Acyl_transf_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWECH_ECOLI
AccessioniPrimary (citable) accession number: P37669
Secondary accession number(s): Q2M7M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.