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Protein

Kdo(2)-lipid A phosphoethanolamine 7''-transferase

Gene

eptB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (Kdo) residue of a Kdo(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases diacylglycerol.1 Publication

Catalytic activityi

Diacylphosphatidylethanolamine + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A = diacyglycerol + 7-O-(2-aminoethoxy(hydroxy)phosphoryl)-alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A.1 Publication
Diacylphosphatidylethanolamine + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid IV(A) = diacyglycerol + 7-O-(2-aminoethoxy(hydroxy)phosphoryl)-alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid IV(A).1 Publication

Cofactori

Ca2+1 Publication

Enzyme regulationi

Inhibited by calcium concentrations higher than 1 mM.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG12267-MONOMER.
ECOL316407:JW5660-MONOMER.
MetaCyc:EG12267-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kdo(2)-lipid A phosphoethanolamine 7''-transferase (EC:2.7.8.421 Publication)
Alternative name(s):
Phosphoethanolamine transferase EptB
Gene namesi
Name:eptB
Synonyms:yhjW
Ordered Locus Names:b3546, JW5660
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12267. eptB.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Topological domaini31 – 48PeriplasmicSequence analysisAdd BLAST18
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 79CytoplasmicSequence analysis10
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Topological domaini101 – 117PeriplasmicSequence analysisAdd BLAST17
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Topological domaini139 – 159CytoplasmicSequence analysisAdd BLAST21
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 563PeriplasmicSequence analysisAdd BLAST383

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002091481 – 563Kdo(2)-lipid A phosphoethanolamine 7''-transferaseAdd BLAST563

Proteomic databases

PaxDbiP37661.
PRIDEiP37661.

Interactioni

Protein-protein interaction databases

BioGridi4262537. 2 interactors.
IntActiP37661. 5 interactors.
STRINGi511145.b3546.

Structurei

3D structure databases

ProteinModelPortaliP37661.
SMRiP37661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DIJ. Bacteria.
COG2194. LUCA.
HOGENOMiHOG000125991.
InParanoidiP37661.
KOiK12975.
OMAiFAMQSEV.
PhylomeDBiP37661.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR012549. DUF1705.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF08019. DUF1705. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

P37661-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYIKSITQQ KLSFLLAIYI GLFMNGAVFY RRFGSYAHDF TVWKGISAVV
60 70 80 90 100
ELAATVLVTF FLLRLLSLFG RRSWRILASL VVLFSAGASY YMTFLNVVIG
110 120 130 140 150
YGIIASVMTT DIDLSKEVVG LNFILWLIAV SALPLILIWN NRCRYTLLRQ
160 170 180 190 200
LRTPGQRIRS LAVVVLAGIM VWAPIRLLDI QQKKVERATG VDLPSYGGVV
210 220 230 240 250
ANSYLPSNWL SALGLYAWAR VDESSDNNSL LNPAKKFTYQ APQNVDDTYV
260 270 280 290 300
VFIIGETTRW DHMGIFGYER NTTPKLAQEK NLAAFRGYSC DTATKLSLRC
310 320 330 340 350
MFVRQGGAED NPQRTLKEQN IFAVLKQLGF SSDLYAMQSE MWFYSNTMAD
360 370 380 390 400
NIAYREQIGA EPRNRGKPVD DMLLVDEMQQ SLGRNPDGKH LIILHTKGSH
410 420 430 440 450
FNYTQRYPRS FAQWKPECIG VDSGCTKAQM INSYDNSVTY VDHFISSVID
460 470 480 490 500
QVRDKKAIVF YAADHGESIN EREHLHGTPR ELAPPEQFRV PMMVWMSDKY
510 520 530 540 550
LENPANAQAF AQLKKEADMK VPRRHVELYD TIMGCLGYTS PDGGINENNN
560
WCHIPQAKEA AAN
Length:563
Mass (Da):63,804
Last modified:December 19, 2001 - v3
Checksum:i40CF0E6A31779230
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18523.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76570.2.
AP009048 Genomic DNA. Translation: BAE77749.1.
PIRiD65153.
RefSeqiNP_418002.2. NC_000913.3.
WP_001269197.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76570; AAC76570; b3546.
BAE77749; BAE77749; BAE77749.
GeneIDi948068.
KEGGiecj:JW5660.
eco:b3546.
PATRICi32122564. VBIEscCol129921_3659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18523.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76570.2.
AP009048 Genomic DNA. Translation: BAE77749.1.
PIRiD65153.
RefSeqiNP_418002.2. NC_000913.3.
WP_001269197.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37661.
SMRiP37661.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262537. 2 interactors.
IntActiP37661. 5 interactors.
STRINGi511145.b3546.

Proteomic databases

PaxDbiP37661.
PRIDEiP37661.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76570; AAC76570; b3546.
BAE77749; BAE77749; BAE77749.
GeneIDi948068.
KEGGiecj:JW5660.
eco:b3546.
PATRICi32122564. VBIEscCol129921_3659.

Organism-specific databases

EchoBASEiEB2176.
EcoGeneiEG12267. eptB.

Phylogenomic databases

eggNOGiENOG4105DIJ. Bacteria.
COG2194. LUCA.
HOGENOMiHOG000125991.
InParanoidiP37661.
KOiK12975.
OMAiFAMQSEV.
PhylomeDBiP37661.

Enzyme and pathway databases

BioCyciEcoCyc:EG12267-MONOMER.
ECOL316407:JW5660-MONOMER.
MetaCyc:EG12267-MONOMER.

Miscellaneous databases

PROiP37661.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR012549. DUF1705.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF08019. DUF1705. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEPTB_ECOLI
AccessioniPrimary (citable) accession number: P37661
Secondary accession number(s): Q2M7K7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 19, 2001
Last modified: November 2, 2016
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.