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Protein

Cyclic di-GMP binding protein BcsE

Gene

bcsE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds bis-(3'-5') cyclic diguanylic acid (c-di-GMP), the ability to bind c-di-GMP is important for its function (PubMed:24942809).1 Publication

Miscellaneous

Cellulose production is abolished in E.coli K12 / MG1655 and W3110 due to a premature stop codon in bcsQ (PubMed:24097954).1 Publication

GO - Molecular functioni

  • cyclic-di-GMP binding Source: EcoCyc

GO - Biological processi

Keywordsi

Biological processCellulose biosynthesis
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12263-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic di-GMP binding protein BcsE1 Publication
Alternative name(s):
Cellulose biosynthesis protein BcsE
Gene namesi
Name:bcsE
Synonyms:yhjS
Ordered Locus Names:b3536, JW3504
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12263 bcsE

Pathology & Biotechi

Disruption phenotypei

When the bcsEFG operon is disrupted in a cellulose-synthesizing strain (a strain K12 / W3110 derivative called AR3110 with a restored, functional bcsQ gene), cellulose is no longer made.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi139R → D: Wild-type c-di-GMP binding, complements a bcsE deletion in S.typhimurium 14028. 1 Publication1
Mutagenesisi287R → D: Wild-type c-di-GMP binding. 1 Publication1
Mutagenesisi306R → D: Wild-type c-di-GMP binding. 1 Publication1
Mutagenesisi364R → D: Wild-type c-di-GMP binding. 1 Publication1
Mutagenesisi415R → D: No longer binds c-di-GMP, does not complement a bcsE deletion in S.typhimurium 14028. 1 Publication1
Mutagenesisi418D → R: No longer binds c-di-GMP. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001695801 – 523Cyclic di-GMP binding protein BcsEAdd BLAST523

Proteomic databases

PaxDbiP37657
PRIDEiP37657

Interactioni

Protein-protein interaction databases

BioGridi4260852, 1 interactor
DIPiDIP-12390N
IntActiP37657, 3 interactors
STRINGi316407.85676509

Structurei

3D structure databases

ProteinModelPortaliP37657
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 492GIL domain, sufficient for c-di-GMP binding1 PublicationAdd BLAST340

Domaini

The central domain (GIL, residues 153-492) is sufficient for c-di-GMP binding.1 Publication

Sequence similaritiesi

Belongs to the BcsE family.Curated

Phylogenomic databases

eggNOGiENOG4105U74 Bacteria
ENOG410ZIFY LUCA
HOGENOMiHOG000125957
OMAiGCGANMV

Family and domain databases

InterProiView protein in InterPro
IPR017745 BcsE_GIL
PfamiView protein in Pfam
PF10995 CBP_GIL, 1 hit
TIGRFAMsiTIGR03369 cellulose_bcsE, 1 hit

Sequencei

Sequence statusi: Complete.

P37657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDIVDPVFS IGISSLWDEL RHMPAGGVWW FNVDRHEDAI SLANQTIASQ
60 70 80 90 100
AETAHVAVIS MDSDPAKIFQ LDDSQGPEKI KLFSMLNHEK GLYYLTRDLQ
110 120 130 140 150
CSIDPHNYLF ILVCANNAWQ NIPAERLRSW LDKMNKWSRL NHCSLLVINP
160 170 180 190 200
GNNNDKQFSL LLEEYRSLFG LASLRFQGDQ HLLDIAFWCN EKGVSARQQL
210 220 230 240 250
SVQQQNGIWT LVQSEEAEIQ PRSDEKRILS NVAVLEGAPP LSEHWQLFNN
260 270 280 290 300
NEVLFNEART AQAATVVFSL QQNAQIEPLA RSIHTLRRQR GSAMKILVRE
310 320 330 340 350
NTASLRATDE RLLLACGANM VIPWNAPLSR CLTMIESVQG QKFSRYVPED
360 370 380 390 400
ITTLLSMTQP LKLRGFQKWD VFCNAVNNMM NNPLLPAHGK GVLVALRPVP
410 420 430 440 450
GIRVEQALTL CRPNRTGDIM TIGGNRLVLF LSFCRINDLD TALNHIFPLP
460 470 480 490 500
TGDIFSNRMV WFEDDQISAE LVQMRLLAPE QWGMPLPLTQ SSKPVINAEH
510 520
DGRHWRRIPE PMRLLDDAVE RSS
Length:523
Mass (Da):59,428
Last modified:October 1, 1994 - v1
Checksum:i4241AF8CE7A9DC35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA Translation: AAB18514.1
U00096 Genomic DNA Translation: AAC76561.1
AP009048 Genomic DNA Translation: BAE77759.1
PIRiS47758
RefSeqiNP_417993.1, NC_000913.3
WP_001204931.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76561; AAC76561; b3536
BAE77759; BAE77759; BAE77759
GeneIDi948050
KEGGiecj:JW3504
eco:b3536
PATRICifig|511145.12.peg.3647

Similar proteinsi

Entry informationi

Entry nameiBCSE_ECOLI
AccessioniPrimary (citable) accession number: P37657
Secondary accession number(s): Q2M7J7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 25, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health