Reviewed, UniProtKB/Swiss-Prot P37653 (BCSA_ECOLI)

Names and origin

Protein names

Recommended name:
    Cellulose synthase catalytic subunit [UDP-forming]
    EC=2.4.1.12

Gene names

Name:	bcsA
Synonyms:	yhjO, yhjP
Ordered Locus Names:	b3533, JW5665

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	872 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalytic subunit of cellulose synthase. It polymerizes uridine 5'-diphosphate glucose to cellulose, which is produced as an extracellular component for mechanical and chemical protection at the onset of the stationary phase, when the cells exhibit multicellular behavior (rdar morphotype). Coexpression of cellulose and thin aggregative fimbriae leads to a hydrophobic network with tightly packed cells embedded in a highly inert matrix.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by bis-(3'-5') cyclic diguanylic acid (c-di-GMP).
Pathway	Glycan metabolism; bacterial cellulose biosynthesis.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Domain	There are two conserved domains in the globular part of the protein: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.
Miscellaneous	The genes bscA, bcsB, bcsZ and bcsC are constitutively transcribed but cellulose synthesis occurs only when adrA, a putative transmembrane protein regulated by agfD, is expressed. Cellulose production is abolished in E.coli K12.
Sequence similarities	Belongs to the glycosyltransferase 2 family.
Sequence caution	The sequence AAB18511.1 differs from that shown. Reason: Frameshift at position 128.

Ontologies

Keywords
Biological process	Cellulose biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	c-di-GMP
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to plasma membrane Inferred from electronic annotation. Source: InterPro
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 872	872	Cellulose synthase catalytic subunit [UDP-forming]		PRO_0000059267
Regions
	Transmembrane	30 – 50	21	Potential
	Transmembrane	151 – 171	21	Potential
	Transmembrane	173 – 193	21	Potential
	Transmembrane	230 – 250	21	Potential
	Transmembrane	525 – 545	21	Potential
	Transmembrane	547 – 567	21	Potential
	Transmembrane	592 – 612	21	Potential
	Transmembrane	640 – 660	21	Potential
	Transmembrane	668 – 688	21	Potential
	Transmembrane	833 – 853	21	Potential
	Region	271 – 364	94	Catalytic subdomain A
	Region	441 – 501	61	Catalytic subdomain B
Sites
	Active site	313	1	Potential
	Active site	457	1	Potential
	Binding site	360	1	Substrate Potential
	Binding site	362	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P37653-1 [UniParc]. Last modified July 26, 2002. Version 3. Checksum: 14326B8A2EB228F7	FASTA	872	99,785
	10 20 30 40 50 60 MSILTRWLLI PPVNARLIGR YRDYRRHGAS AFSATLGCFW MILAWIFIPL EHPRWQRIRA 70 80 90 100 110 120 EHKNLYPHIN ASRPRPLDPV RYLIQTCWLL IGASRKETPK PRRRAFSGLQ NIRGRYHQWM 130 140 150 160 170 180 NELPERVSHK TQHLDEKKEL GHLSAGARRL ILGIIVTFSL ILALICVTQP FNPLAQFIFL 190 200 210 220 230 240 MLLWGVALIV RRMPGRFSAL MLIVLSLTVS CRYIWWRYTS TLNWDDPVSL VCGLILLFAE 250 260 270 280 290 300 TYAWIVLVLG YFQVVWPLNR QPVPLPKDMS LWPSVDIFVP TYNEDLNVVK NTIYASLGID 310 320 330 340 350 360 WPKDKLNIWI LDDGGREEFR QFAQNVGVKY IARTTHEHAK AGNINNALKY AKGEFVSIFD 370 380 390 400 410 420 CDHVPTRSFL QMTMGWFLKE KQLAMMQTPH HFFSPDPFER NLGRFRKTPN EGTLFYGLVQ 430 440 450 460 470 480 DGNDMWDATF FCGSCAVIRR KPLDEIGGIA VETVTEDAHT SLRLHRRGYT SAYMRIPQAA 490 500 510 520 530 540 GLATESLSAH IGQRIRWARG MVQIFRLDNP LTGKGLKFAQ RLCYVNAMFH FLSGIPRLIF 550 560 570 580 590 600 LTAPLAFLLL HAYIIYAPAL MIALFVLPHM IHASLTNSKI QGKYRHSFWS EIYETVLAWY 610 620 630 640 650 660 IAPPTLVALI NPHKGKFNVT AKGGLVEEEY VDWVISRPYI FLVLLNLVGV AVGIWRYFYG 670 680 690 700 710 720 PPTEMLTVVV SMVWVFYNLI VLGGAVAVSV ESKQVRRSHR VEMTMPAAIA REDGHLFSCT 730 740 750 760 770 780 VQDFSDGGLG IKINGQAQIL EGQKVNLLLK RGQQEYVFPT QVARVMGNEV GLKLMPLTTQ 790 800 810 820 830 840 QHIDFVQCTF ARADTWALWQ DSYPEDKPLE SLLDILKLGF RGYRHLAEFA PSSVKGIFRV 850 860 870 LTSLVSWVVS FIPRRPERSE TAQPSDQALA QQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix." Zogaj X., Nimtz M., Rohde M., Bokranz W., Roemling U. Mol. Microbiol. 39:1452-1463(2001) [PubMed: 11260463] [Abstract] Cited for: CHARACTERIZATION. Strain: ECOR 10, ECOR 12 and TOB1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00039 Genomic DNA. Translation: AAB18510.1. Frameshift. U00039 Genomic DNA. Translation: AAB18511.1. Frameshift. U00096 Genomic DNA. Translation: AAC76558.1. Different initiation. AP009048 Genomic DNA. Translation: BAE77761.1.
PIR	H65151. S47754. S47755.
RefSeq	AP_004260.1. NP_417990.4.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:12387N.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	948053.
GenomeReviews	Gene locus JW5665 in contig AP009048_GR. Gene locus b3533 in contig U00096_GR.
KEGG	ecj:JW5665. eco:b3533.
Organism-specific databases
EchoBASE	EB2169.
EcoGene	EG12260. bcsA.
CMR	Search...
Phylogenomic databases
HOGENOM	P37653.
OMA	P37653. CYANAML.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12260-MON.
Family and domain databases
InterPro	IPR003919. Cell_synth_A. IPR017480. Cellulose_synth_catalytic. IPR001173. Glyco_trans_2. IPR009875. PilZ. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. PF07238. PilZ. 1 hit. [Graphical view]
PRINTS	PR01439. CELLSNTHASEA.
TIGRFAMs	TIGR03030. CelA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BCSA_ECOLI

Accession

Primary (citable) accession number: P37653
Secondary accession number(s): P37654 , P76712, P76713, Q2M7J5, Q8RSS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 26, 2002
Last modified:	June 16, 2009
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)