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Protein

Cyclic di-GMP-binding protein

Gene

bcsB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds the cellulose synthase activator, bis-(3'-5') cyclic diguanylic acid (c-di-GMP).By similarity

Miscellaneous

The genes bscA, bcsB, bcsZ and bcsC are constitutively transcribed but cellulose synthesis occurs only when AdrA, a putative transmembrane protein regulated by AgfD, is expressed. Cellulose production is abolished in E.coli K12.

Pathwayi: bacterial cellulose biosynthesis

This protein is involved in the pathway bacterial cellulose biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway bacterial cellulose biosynthesis and in Glycan metabolism.

GO - Biological processi

Keywordsi

Biological processCellulose biosynthesis
Ligandc-di-GMP

Enzyme and pathway databases

BioCyciEcoCyc:EG12259-MONOMER.
MetaCyc:EG12259-MONOMER.
UniPathwayiUPA00694.

Protein family/group databases

TCDBi4.D.3.1.6. the glycan glucosyl transferase (opgh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic di-GMP-binding protein
Alternative name(s):
Cellulose synthase regulatory subunit
Gene namesi
Name:bcsB
Synonyms:yhjN
Ordered Locus Names:b3532, JW3500
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12259. bcsB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 742PeriplasmicSequence analysisAdd BLAST717
Transmembranei743 – 763HelicalSequence analysisAdd BLAST21
Topological domaini764 – 779CytoplasmicSequence analysisAdd BLAST16

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000026826 – 779Cyclic di-GMP-binding proteinAdd BLAST754

Proteomic databases

PaxDbiP37652.
PRIDEiP37652.

Interactioni

Subunit structurei

Tightly associated with the cellulose synthase catalytic subunit.By similarity

Protein-protein interaction databases

BioGridi4262529. 269 interactors.
DIPiDIP-12386N.
IntActiP37652. 4 interactors.
STRINGi316385.ECDH10B_3709.

Structurei

3D structure databases

ProteinModelPortaliP37652.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AcsB/BcsB family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107EEX. Bacteria.
ENOG410XNNB. LUCA.
HOGENOMiHOG000259077.
InParanoidiP37652.
KOiK20541.

Family and domain databases

InterProiView protein in InterPro
IPR003920. Cell_synth_B.
IPR018513. Cell_synthase_bac.
PfamiView protein in Pfam
PF03170. BcsB. 1 hit.
PRINTSiPR01440. CELLSNTHASEB.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKLFWICA VAMGMSAFPS FMTQATPATQ PLINAEPAVA AQTEQNPQVG
60 70 80 90 100
QVMPGVQGAD APVVAQNGPS RDVKLTFAQI APPPGSMVLR GINPNGSIEF
110 120 130 140 150
GMRSDEVVTK AMLNLEYTPS PSLLPVQSQL KVYLNDELMG VLPVTKEQLG
160 170 180 190 200
KKTLAQMPIN PLFISDFNRV RLEFVGHYQD VCEKPASTTL WLDVGRSSGL
210 220 230 240 250
DLTYQTLNVK NDLSHFPVPF FDPSDNRTNT LPMVFAGAPD VGLQQASAIV
260 270 280 290 300
ASWFGSRSGW RGQNFPVLYN QLPDRNAIVF ATNDKRPDFL RDHPAVKAPV
310 320 330 340 350
IEMINHPQNP YVKLLVVFGR DDKDLLQAAK GIAQGNILFR GESVVVNEVK
360 370 380 390 400
PLLPRKPYDA PNWVRTDRPV TFGELKTYEE QLQSSGLEPA AINVSLNLPP
410 420 430 440 450
DLYLMRSTGI DMDINYRYTM PPVKDSSRMD ISLNNQFLQS FNLSSKQEAN
460 470 480 490 500
RLLLRIPVLQ GLLDGKTDVS IPALKLGATN QLRFDFEYMN PMPGGSVDNC
510 520 530 540 550
ITFQPVQNHV VIGDDSTIDF SKYYHFIPMP DLRAFANAGF PFSRMADLSQ
560 570 580 590 600
TITVMPKAPN EAQMETLLNT VGFIGAQTGF PAINLTVTDD GSTIQGKDAD
610 620 630 640 650
IMIIGGIPDK LKDDKQIDLL VQATESWVKT PMRQTPFPGI VPDESDRAAE
660 670 680 690 700
TRSTLTSSGA MAAVIGFQSP YNDQRSVIAL LADSPRGYEM LNDAVNDSGK
710 720 730 740 750
RATMFGSVAV IRESGINSLR VGDVYYVGHL PWFERVWYAL ANHPILLAVL
760 770
AAISVILLAW VLWRLLRIIS RRRLNPDNE
Length:779
Mass (Da):86,024
Last modified:December 1, 2000 - v2
Checksum:iF3188171DDC23918
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18509.1.
U00096 Genomic DNA. Translation: AAC76557.1.
AP009048 Genomic DNA. Translation: BAE77762.1.
PIRiG65151.
RefSeqiNP_417989.1. NC_000913.3.
WP_001407405.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76557; AAC76557; b3532.
BAE77762; BAE77762; BAE77762.
GeneIDi948045.
KEGGiecj:JW3500.
eco:b3532.
PATRICifig|511145.12.peg.3643.

Similar proteinsi

Entry informationi

Entry nameiBCSB_ECOLI
AccessioniPrimary (citable) accession number: P37652
Secondary accession number(s): P76711, Q2M7J4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 1, 2000
Last modified: August 30, 2017
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families