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P37651

- GUN_ECOLI

UniProt

P37651 - GUN_ECOLI

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Protein
Endoglucanase
Gene
bcsZ, bcsC, yhjM, b3531, JW3499
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes carboxymethylcellulose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei55 – 551Proton donor By similarity
Active sitei116 – 1161Nucleophile Reviewed prediction

GO - Molecular functioni

  1. cellulase activity Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciEcoCyc:EG12258-MONOMER.
ECOL316407:JW3499-MONOMER.
MetaCyc:EG12258-MONOMER.
UniPathwayiUPA00694.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Carboxymethylcellulase
Short name:
CMCase
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:bcsZ
Synonyms:bcsC, yhjM
Ordered Locus Names:b3531, JW3499
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12258. bcsZ.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 368347Endoglucanase
PRO_0000007938Add
BLAST

Proteomic databases

PaxDbiP37651.
PRIDEiP37651.

Expressioni

Gene expression databases

GenevestigatoriP37651.

Interactioni

Protein-protein interaction databases

IntActiP37651. 3 interactions.
STRINGi511145.b3531.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 3510
Beta strandi51 – 533
Helixi54 – 6613
Helixi70 – 8415
Turni89 – 913
Beta strandi96 – 1005
Beta strandi106 – 1105
Helixi115 – 13117
Helixi135 – 15218
Beta strandi153 – 1564
Turni157 – 1593
Beta strandi160 – 1634
Beta strandi165 – 1728
Beta strandi175 – 1784
Helixi185 – 1917
Helixi192 – 1943
Helixi198 – 21114
Beta strandi220 – 2256
Turni226 – 2283
Beta strandi239 – 2413
Turni242 – 2443
Helixi245 – 2528
Helixi259 – 2679
Helixi269 – 27810
Beta strandi283 – 2864
Turni287 – 2893
Beta strandi292 – 2943
Helixi298 – 30710
Helixi311 – 32313
Helixi331 – 34414
Beta strandi347 – 3504
Beta strandi356 – 3583

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QXFX-ray1.85A/B/C/D22-368[»]
3QXQX-ray2.20A/B/C/D22-368[»]
ProteinModelPortaliP37651.
SMRiP37651. Positions 24-360.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3405.
HOGENOMiHOG000259088.
KOiK01179.
OMAiDLWIAYT.
OrthoDBiEOG6DG2PM.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37651-1 [UniParc]FASTAAdd to Basket

« Hide

MNVLRSGIVT MLLLAAFSVQ AACTWPAWEQ FKKDYISQEG RVIDPSDARK    50
ITTSEGQSYG MFSALAANDR AAFDNILDWT QNNLAQGSLK ERLPAWLWGK 100
KENSKWEVLD SNSASDGDVW MAWSLLEAGR LWKEQRYTDI GSALLKRIAR 150
EEVVTVPGLG SMLLPGKVGF AEDNSWRFNP SYLPPTLAQY FTRFGAPWTT 200
LRETNQRLLL ETAPKGFSPD WVRYEKDKGW QLKAEKTLIS SYDAIRVYMW 250
VGMMPDSDPQ KARMLNRFKP MATFTEKNGY PPEKVDVATG KAQGKGPVGF 300
SAAMLPFLQN RDAQAVQRQR VADNFPGSDA YYNYVLTLFG QGWDQHRFRF 350
STKGELLPDW GQECANSH 368
Length:368
Mass (Da):41,700
Last modified:October 1, 1994 - v1
Checksum:i6539C03D4D0CFDEA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00039 Genomic DNA. Translation: AAB18508.1.
U00096 Genomic DNA. Translation: AAC76556.1.
AP009048 Genomic DNA. Translation: BAE77763.1.
PIRiS47752.
RefSeqiNP_417988.1. NC_000913.3.
YP_491904.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76556; AAC76556; b3531.
BAE77763; BAE77763; BAE77763.
GeneIDi12934278.
948046.
KEGGiecj:Y75_p3646.
eco:b3531.
PATRICi32122528. VBIEscCol129921_3642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00039 Genomic DNA. Translation: AAB18508.1 .
U00096 Genomic DNA. Translation: AAC76556.1 .
AP009048 Genomic DNA. Translation: BAE77763.1 .
PIRi S47752.
RefSeqi NP_417988.1. NC_000913.3.
YP_491904.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3QXF X-ray 1.85 A/B/C/D 22-368 [» ]
3QXQ X-ray 2.20 A/B/C/D 22-368 [» ]
ProteinModelPortali P37651.
SMRi P37651. Positions 24-360.
ModBasei Search...

Protein-protein interaction databases

IntActi P37651. 3 interactions.
STRINGi 511145.b3531.

Protein family/group databases

CAZyi GH8. Glycoside Hydrolase Family 8.

Proteomic databases

PaxDbi P37651.
PRIDEi P37651.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76556 ; AAC76556 ; b3531 .
BAE77763 ; BAE77763 ; BAE77763 .
GeneIDi 12934278.
948046.
KEGGi ecj:Y75_p3646.
eco:b3531.
PATRICi 32122528. VBIEscCol129921_3642.

Organism-specific databases

EchoBASEi EB2167.
EcoGenei EG12258. bcsZ.

Phylogenomic databases

eggNOGi COG3405.
HOGENOMi HOG000259088.
KOi K01179.
OMAi DLWIAYT.
OrthoDBi EOG6DG2PM.

Enzyme and pathway databases

UniPathwayi UPA00694 .
BioCyci EcoCyc:EG12258-MONOMER.
ECOL316407:JW3499-MONOMER.
MetaCyc:EG12258-MONOMER.

Miscellaneous databases

PROi P37651.

Gene expression databases

Genevestigatori P37651.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view ]
Pfami PF01270. Glyco_hydro_8. 1 hit.
[Graphical view ]
PRINTSi PR00735. GLHYDRLASE8.
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the Escherichia coli endo-1,4-D-glucanase gene and identification of its product."
    Park Y.W., Yun H.D.
    Mol. Gen. Genet. 261:236-241(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix."
    Zogaj X., Nimtz M., Rohde M., Bokranz W., Roemling U.
    Mol. Microbiol. 39:1452-1463(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ECOR 10, ECOR 12 and TOB1.

Entry informationi

Entry nameiGUN_ECOLI
AccessioniPrimary (citable) accession number: P37651
Secondary accession number(s): Q2M7J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 14, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The genes bscA, bcsB, bcsZ and bcsC are constitutively transcribed but cellulose synthesis occurs only when AdrA, a putative transmembrane protein regulated by AgfD, is expressed. Cellulose production is abolished in E.coli K12.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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