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P37651 (GUN_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase

EC=3.2.1.4
Alternative name(s):
Carboxymethylcellulase
Short name=CMCase
Cellulase
Endo-1,4-beta-glucanase
Gene names
Name:bcsZ
Synonyms:bcsC, yhjM
Ordered Locus Names:b3531, JW3499
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes carboxymethylcellulose.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathway

Glycan metabolism; bacterial cellulose biosynthesis.

Subcellular location

Secreted.

Miscellaneous

The genes bscA, bcsB, bcsZ and bcsC are constitutively transcribed but cellulose synthesis occurs only when AdrA, a putative transmembrane protein regulated by AgfD, is expressed. Cellulose production is abolished in E.coli K12.

Sequence similarities

Belongs to the glycosyl hydrolase 8 (cellulase D) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from direct assay Ref.1. Source: EcoCyc

   Molecular_functioncellulase activity

Inferred from direct assay Ref.1. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 368347Endoglucanase
PRO_0000007938

Sites

Active site551Proton donor By similarity
Active site1161Nucleophile Potential

Secondary structure

........................................................ 368
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P37651 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 6539C03D4D0CFDEA

FASTA36841,700
        10         20         30         40         50         60 
MNVLRSGIVT MLLLAAFSVQ AACTWPAWEQ FKKDYISQEG RVIDPSDARK ITTSEGQSYG 

        70         80         90        100        110        120 
MFSALAANDR AAFDNILDWT QNNLAQGSLK ERLPAWLWGK KENSKWEVLD SNSASDGDVW 

       130        140        150        160        170        180 
MAWSLLEAGR LWKEQRYTDI GSALLKRIAR EEVVTVPGLG SMLLPGKVGF AEDNSWRFNP 

       190        200        210        220        230        240 
SYLPPTLAQY FTRFGAPWTT LRETNQRLLL ETAPKGFSPD WVRYEKDKGW QLKAEKTLIS 

       250        260        270        280        290        300 
SYDAIRVYMW VGMMPDSDPQ KARMLNRFKP MATFTEKNGY PPEKVDVATG KAQGKGPVGF 

       310        320        330        340        350        360 
SAAMLPFLQN RDAQAVQRQR VADNFPGSDA YYNYVLTLFG QGWDQHRFRF STKGELLPDW 


GQECANSH 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the Escherichia coli endo-1,4-D-glucanase gene and identification of its product."
Park Y.W., Yun H.D.
Mol. Gen. Genet. 261:236-241(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix."
Zogaj X., Nimtz M., Rohde M., Bokranz W., Roemling U.
Mol. Microbiol. 39:1452-1463(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: ECOR 10, ECOR 12 and TOB1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00039 Genomic DNA. Translation: AAB18508.1.
U00096 Genomic DNA. Translation: AAC76556.1.
AP009048 Genomic DNA. Translation: BAE77763.1.
PIRS47752.
RefSeqNP_417988.1. NC_000913.3.
YP_491904.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3QXFX-ray1.85A/B/C/D22-368[»]
3QXQX-ray2.20A/B/C/D22-368[»]
ProteinModelPortalP37651.
SMRP37651. Positions 24-360.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP37651. 3 interactions.
STRING511145.b3531.

Protein family/group databases

CAZyGH8. Glycoside Hydrolase Family 8.

Proteomic databases

PaxDbP37651.
PRIDEP37651.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76556; AAC76556; b3531.
BAE77763; BAE77763; BAE77763.
GeneID12934278.
948046.
KEGGecj:Y75_p3646.
eco:b3531.
PATRIC32122528. VBIEscCol129921_3642.

Organism-specific databases

EchoBASEEB2167.
EcoGeneEG12258. bcsZ.

Phylogenomic databases

eggNOGCOG3405.
HOGENOMHOG000259088.
KOK01179.
OMAIRVYLWV.
OrthoDBEOG6DG2PM.
ProtClustDBPRK11097.

Enzyme and pathway databases

BioCycEcoCyc:EG12258-MONOMER.
ECOL316407:JW3499-MONOMER.
MetaCyc:EG12258-MONOMER.
UniPathwayUPA00694.

Gene expression databases

GenevestigatorP37651.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSPR00735. GLHYDRLASE8.
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP37651.

Entry information

Entry nameGUN_ECOLI
AccessionPrimary (citable) accession number: P37651
Secondary accession number(s): Q2M7J3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: March 19, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene