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Protein

Endoglucanase

Gene

bcsZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes carboxymethylcellulose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei55 – 551Proton donorBy similarity
Active sitei116 – 1161NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  • cellulase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciEcoCyc:EG12258-MONOMER.
ECOL316407:JW3499-MONOMER.
MetaCyc:EG12258-MONOMER.
UniPathwayiUPA00694.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Carboxymethylcellulase
Short name:
CMCase
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:bcsZ
Synonyms:bcsC, yhjM
Ordered Locus Names:b3531, JW3499
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12258. bcsZ.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 368347EndoglucanasePRO_0000007938Add
BLAST

Proteomic databases

PaxDbiP37651.
PRIDEiP37651.

Expressioni

Gene expression databases

GenevestigatoriP37651.

Interactioni

Protein-protein interaction databases

IntActiP37651. 3 interactions.
STRINGi511145.b3531.

Structurei

Secondary structure

1
368
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 3510Combined sources
Beta strandi51 – 533Combined sources
Helixi54 – 6613Combined sources
Helixi70 – 8415Combined sources
Turni89 – 913Combined sources
Beta strandi96 – 1005Combined sources
Beta strandi106 – 1105Combined sources
Helixi115 – 13117Combined sources
Helixi135 – 15218Combined sources
Beta strandi153 – 1564Combined sources
Turni157 – 1593Combined sources
Beta strandi160 – 1634Combined sources
Beta strandi165 – 1728Combined sources
Beta strandi175 – 1784Combined sources
Helixi185 – 1917Combined sources
Helixi192 – 1943Combined sources
Helixi198 – 21114Combined sources
Beta strandi220 – 2256Combined sources
Turni226 – 2283Combined sources
Beta strandi239 – 2413Combined sources
Turni242 – 2443Combined sources
Helixi245 – 2528Combined sources
Helixi259 – 2679Combined sources
Helixi269 – 27810Combined sources
Beta strandi283 – 2864Combined sources
Turni287 – 2893Combined sources
Beta strandi292 – 2943Combined sources
Helixi298 – 30710Combined sources
Helixi311 – 32313Combined sources
Helixi331 – 34414Combined sources
Beta strandi347 – 3504Combined sources
Beta strandi356 – 3583Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QXFX-ray1.85A/B/C/D22-368[»]
3QXQX-ray2.20A/B/C/D22-368[»]
ProteinModelPortaliP37651.
SMRiP37651. Positions 24-360.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3405.
HOGENOMiHOG000259088.
InParanoidiP37651.
KOiK01179.
OMAiIRVYLWV.
OrthoDBiEOG6DG2PM.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLRSGIVT MLLLAAFSVQ AACTWPAWEQ FKKDYISQEG RVIDPSDARK
60 70 80 90 100
ITTSEGQSYG MFSALAANDR AAFDNILDWT QNNLAQGSLK ERLPAWLWGK
110 120 130 140 150
KENSKWEVLD SNSASDGDVW MAWSLLEAGR LWKEQRYTDI GSALLKRIAR
160 170 180 190 200
EEVVTVPGLG SMLLPGKVGF AEDNSWRFNP SYLPPTLAQY FTRFGAPWTT
210 220 230 240 250
LRETNQRLLL ETAPKGFSPD WVRYEKDKGW QLKAEKTLIS SYDAIRVYMW
260 270 280 290 300
VGMMPDSDPQ KARMLNRFKP MATFTEKNGY PPEKVDVATG KAQGKGPVGF
310 320 330 340 350
SAAMLPFLQN RDAQAVQRQR VADNFPGSDA YYNYVLTLFG QGWDQHRFRF
360
STKGELLPDW GQECANSH
Length:368
Mass (Da):41,700
Last modified:October 1, 1994 - v1
Checksum:i6539C03D4D0CFDEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18508.1.
U00096 Genomic DNA. Translation: AAC76556.1.
AP009048 Genomic DNA. Translation: BAE77763.1.
PIRiS47752.
RefSeqiNP_417988.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76556; AAC76556; b3531.
BAE77763; BAE77763; BAE77763.
GeneIDi948046.
KEGGiecj:Y75_p3646.
eco:b3531.
PATRICi32122528. VBIEscCol129921_3642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18508.1.
U00096 Genomic DNA. Translation: AAC76556.1.
AP009048 Genomic DNA. Translation: BAE77763.1.
PIRiS47752.
RefSeqiNP_417988.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QXFX-ray1.85A/B/C/D22-368[»]
3QXQX-ray2.20A/B/C/D22-368[»]
ProteinModelPortaliP37651.
SMRiP37651. Positions 24-360.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP37651. 3 interactions.
STRINGi511145.b3531.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Proteomic databases

PaxDbiP37651.
PRIDEiP37651.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76556; AAC76556; b3531.
BAE77763; BAE77763; BAE77763.
GeneIDi948046.
KEGGiecj:Y75_p3646.
eco:b3531.
PATRICi32122528. VBIEscCol129921_3642.

Organism-specific databases

EchoBASEiEB2167.
EcoGeneiEG12258. bcsZ.

Phylogenomic databases

eggNOGiCOG3405.
HOGENOMiHOG000259088.
InParanoidiP37651.
KOiK01179.
OMAiIRVYLWV.
OrthoDBiEOG6DG2PM.

Enzyme and pathway databases

UniPathwayiUPA00694.
BioCyciEcoCyc:EG12258-MONOMER.
ECOL316407:JW3499-MONOMER.
MetaCyc:EG12258-MONOMER.

Miscellaneous databases

PROiP37651.

Gene expression databases

GenevestigatoriP37651.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the Escherichia coli endo-1,4-D-glucanase gene and identification of its product."
    Park Y.W., Yun H.D.
    Mol. Gen. Genet. 261:236-241(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix."
    Zogaj X., Nimtz M., Rohde M., Bokranz W., Roemling U.
    Mol. Microbiol. 39:1452-1463(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ECOR 10, ECOR 12 and TOB1.

Entry informationi

Entry nameiGUN_ECOLI
AccessioniPrimary (citable) accession number: P37651
Secondary accession number(s): Q2M7J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 27, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The genes bscA, bcsB, bcsZ and bcsC are constitutively transcribed but cellulose synthesis occurs only when AdrA, a putative transmembrane protein regulated by AgfD, is expressed. Cellulose production is abolished in E.coli K12.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.