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Protein

Endoglucanase

Gene

bcsZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes carboxymethylcellulose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayi: bacterial cellulose biosynthesis

This protein is involved in the pathway bacterial cellulose biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway bacterial cellulose biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei55Proton donorBy similarity1
Active sitei116NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

  • cellulase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciEcoCyc:EG12258-MONOMER.
ECOL316407:JW3499-MONOMER.
MetaCyc:EG12258-MONOMER.
UniPathwayiUPA00694.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.
TCDBi4.D.3.1.6. the glycan glucosyl transferase (opgh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Carboxymethylcellulase
Short name:
CMCase
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:bcsZ
Synonyms:bcsC, yhjM
Ordered Locus Names:b3531, JW3499
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12258. bcsZ.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000793822 – 368EndoglucanaseAdd BLAST347

Proteomic databases

PaxDbiP37651.
PRIDEiP37651.

Interactioni

Protein-protein interaction databases

BioGridi4263272. 99 interactors.
IntActiP37651. 4 interactors.
STRINGi511145.b3531.

Structurei

Secondary structure

1368
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 35Combined sources10
Beta strandi51 – 53Combined sources3
Helixi54 – 66Combined sources13
Helixi70 – 84Combined sources15
Turni89 – 91Combined sources3
Beta strandi96 – 100Combined sources5
Beta strandi106 – 110Combined sources5
Helixi115 – 131Combined sources17
Helixi135 – 152Combined sources18
Beta strandi153 – 156Combined sources4
Turni157 – 159Combined sources3
Beta strandi160 – 163Combined sources4
Beta strandi165 – 172Combined sources8
Beta strandi175 – 178Combined sources4
Helixi185 – 191Combined sources7
Helixi192 – 194Combined sources3
Helixi198 – 211Combined sources14
Beta strandi220 – 225Combined sources6
Turni226 – 228Combined sources3
Beta strandi239 – 241Combined sources3
Turni242 – 244Combined sources3
Helixi245 – 252Combined sources8
Helixi259 – 267Combined sources9
Helixi269 – 278Combined sources10
Beta strandi283 – 286Combined sources4
Turni287 – 289Combined sources3
Beta strandi292 – 294Combined sources3
Helixi298 – 307Combined sources10
Helixi311 – 323Combined sources13
Helixi331 – 344Combined sources14
Beta strandi347 – 350Combined sources4
Beta strandi356 – 358Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QXFX-ray1.85A/B/C/D22-368[»]
3QXQX-ray2.20A/B/C/D22-368[»]
ProteinModelPortaliP37651.
SMRiP37651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105K2Z. Bacteria.
COG3405. LUCA.
HOGENOMiHOG000259088.
InParanoidiP37651.
KOiK20542.
OMAiIRVYLWV.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLRSGIVT MLLLAAFSVQ AACTWPAWEQ FKKDYISQEG RVIDPSDARK
60 70 80 90 100
ITTSEGQSYG MFSALAANDR AAFDNILDWT QNNLAQGSLK ERLPAWLWGK
110 120 130 140 150
KENSKWEVLD SNSASDGDVW MAWSLLEAGR LWKEQRYTDI GSALLKRIAR
160 170 180 190 200
EEVVTVPGLG SMLLPGKVGF AEDNSWRFNP SYLPPTLAQY FTRFGAPWTT
210 220 230 240 250
LRETNQRLLL ETAPKGFSPD WVRYEKDKGW QLKAEKTLIS SYDAIRVYMW
260 270 280 290 300
VGMMPDSDPQ KARMLNRFKP MATFTEKNGY PPEKVDVATG KAQGKGPVGF
310 320 330 340 350
SAAMLPFLQN RDAQAVQRQR VADNFPGSDA YYNYVLTLFG QGWDQHRFRF
360
STKGELLPDW GQECANSH
Length:368
Mass (Da):41,700
Last modified:October 1, 1994 - v1
Checksum:i6539C03D4D0CFDEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18508.1.
U00096 Genomic DNA. Translation: AAC76556.1.
AP009048 Genomic DNA. Translation: BAE77763.1.
PIRiS47752.
RefSeqiNP_417988.1. NC_000913.3.
WP_001311203.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76556; AAC76556; b3531.
BAE77763; BAE77763; BAE77763.
GeneIDi948046.
KEGGiecj:JW3499.
eco:b3531.
PATRICi32122528. VBIEscCol129921_3642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18508.1.
U00096 Genomic DNA. Translation: AAC76556.1.
AP009048 Genomic DNA. Translation: BAE77763.1.
PIRiS47752.
RefSeqiNP_417988.1. NC_000913.3.
WP_001311203.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QXFX-ray1.85A/B/C/D22-368[»]
3QXQX-ray2.20A/B/C/D22-368[»]
ProteinModelPortaliP37651.
SMRiP37651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263272. 99 interactors.
IntActiP37651. 4 interactors.
STRINGi511145.b3531.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.
TCDBi4.D.3.1.6. the glycan glucosyl transferase (opgh) family.

Proteomic databases

PaxDbiP37651.
PRIDEiP37651.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76556; AAC76556; b3531.
BAE77763; BAE77763; BAE77763.
GeneIDi948046.
KEGGiecj:JW3499.
eco:b3531.
PATRICi32122528. VBIEscCol129921_3642.

Organism-specific databases

EchoBASEiEB2167.
EcoGeneiEG12258. bcsZ.

Phylogenomic databases

eggNOGiENOG4105K2Z. Bacteria.
COG3405. LUCA.
HOGENOMiHOG000259088.
InParanoidiP37651.
KOiK20542.
OMAiIRVYLWV.

Enzyme and pathway databases

UniPathwayiUPA00694.
BioCyciEcoCyc:EG12258-MONOMER.
ECOL316407:JW3499-MONOMER.
MetaCyc:EG12258-MONOMER.

Miscellaneous databases

PROiP37651.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUN_ECOLI
AccessioniPrimary (citable) accession number: P37651
Secondary accession number(s): Q2M7J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The genes bscA, bcsB, bcsZ and bcsC are constitutively transcribed but cellulose synthesis occurs only when AdrA, a putative transmembrane protein regulated by AgfD, is expressed. Cellulose production is abolished in E.coli K12.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.