Reviewed, UniProtKB/Swiss-Prot P37651 (GUN_ECOLI)

Names and origin

Protein names

Recommended name:
    Endoglucanase
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase
    Cellulase
    Carboxymethylcellulase
      Short name=CMCase

Gene names

Name:	bcsZ
Synonyms:	bcsC, yhjM
Ordered Locus Names:	b3531, JW3499

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes carboxymethylcellulose.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Pathway	Glycan metabolism; bacterial cellulose biosynthesis.
Subcellular location	Secreted.
Miscellaneous	The genes bscA, bcsB, bcsZ and bcsC are constitutively transcribed but cellulose synthesis occurs only when adrA, a putative transmembrane protein regulated by agfD, is expressed. Cellulose production is abolished in E.coli K12.
Sequence similarities	Belongs to the glycosyl hydrolase 8 (cellulase D) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P19777	1	EBI-1114108,EBI-1133014

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 368	347	Endoglucanase		PRO_0000007938
Sites
	Active site	55	1	Proton donor By similarity
	Active site	116	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	P37651-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 6539C03D4D0CFDEA	FASTA	368	41,700
	10 20 30 40 50 60 MNVLRSGIVT MLLLAAFSVQ AACTWPAWEQ FKKDYISQEG RVIDPSDARK ITTSEGQSYG 70 80 90 100 110 120 MFSALAANDR AAFDNILDWT QNNLAQGSLK ERLPAWLWGK KENSKWEVLD SNSASDGDVW 130 140 150 160 170 180 MAWSLLEAGR LWKEQRYTDI GSALLKRIAR EEVVTVPGLG SMLLPGKVGF AEDNSWRFNP 190 200 210 220 230 240 SYLPPTLAQY FTRFGAPWTT LRETNQRLLL ETAPKGFSPD WVRYEKDKGW QLKAEKTLIS 250 260 270 280 290 300 SYDAIRVYMW VGMMPDSDPQ KARMLNRFKP MATFTEKNGY PPEKVDVATG KAQGKGPVGF 310 320 330 340 350 360 SAAMLPFLQN RDAQAVQRQR VADNFPGSDA YYNYVLTLFG QGWDQHRFRF STKGELLPDW GQECANSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the Escherichia coli endo-1,4-D-glucanase gene and identification of its product." Park Y.W., Yun H.D. Mol. Gen. Genet. 261:236-241(1999) [PubMed: 10102357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix." Zogaj X., Nimtz M., Rohde M., Bokranz W., Roemling U. Mol. Microbiol. 39:1452-1463(2001) [PubMed: 11260463] [Abstract] Cited for: CHARACTERIZATION. Strain: ECOR 10, ECOR 12 and TOB1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00039 Genomic DNA. Translation: AAB18508.1. U00096 Genomic DNA. Translation: AAC76556.1. AP009048 Genomic DNA. Translation: BAE77763.1.
PIR	S47752.
RefSeq	AP_004262.1. NP_417988.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P37651. 4 interactions.
Protein family/group databases
CAZy	GH8. Glycoside Hydrolase Family 8.
Genome annotation databases
GeneID	948046.
GenomeReviews	Gene locus JW3499 in contig AP009048_GR. Gene locus b3531 in contig U00096_GR.
KEGG	ecj:JW3499. eco:b3531.
Organism-specific databases
EchoBASE	EB2167.
EcoGene	EG12258. bcsZ.
CMR	Search...
Phylogenomic databases
HOGENOM	P37651.
OMA	P37651. GSYDAIR.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12258-MON. MetaCyc:EG12258-MON.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR002037. Glyco_hydro_8. IPR019834. Glyco_hydro_8_CS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
Pfam	PF01270. Glyco_hydro_8. 1 hit. [Graphical view]
PRINTS	PR00735. GLHYDRLASE8.
PROSITE	PS00812. GLYCOSYL_HYDROL_F8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_ECOLI

Accession

Primary (citable) accession number: P37651
Secondary accession number(s): Q2M7J3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)