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Protein

Probable cyclic di-GMP phosphodiesterase PdeK

Gene

pdeK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG.By similarity

Catalytic activityi

Cyclic di-3',5'-guanylate + H2O = 5'-phosphoguanylyl(3'->5')guanosine.By similarity

GO - Molecular functioni

  • cyclic-guanylate-specific phosphodiesterase activity Source: UniProtKB-EC
  • cyclic-nucleotide phosphodiesterase activity Source: EcoCyc

GO - Biological processi

  • 3',5'-cyclic diguanylic acid metabolic process Source: EcoCyc
  • signal transduction Source: InterPro

Keywordsi

Molecular functionHydrolase
Ligandc-di-GMP

Enzyme and pathway databases

BioCyciEcoCyc:EG12256-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cyclic di-GMP phosphodiesterase PdeKCurated (EC:3.1.4.52By similarity)
Gene namesi
Name:pdeK1 Publication
Synonyms:yhjK
Ordered Locus Names:b3529, JW5943
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12256 yhjK

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001695741 – 662Probable cyclic di-GMP phosphodiesterase PdeKAdd BLAST662

Proteomic databases

PaxDbiP37649
PRIDEiP37649

Expressioni

Inductioni

Expressed at 28 degrees Celsius in stationary phase.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
hscCP773193EBI-562146,EBI-562084

Protein-protein interaction databases

BioGridi4262160, 7 interactors
DIPiDIP-12384N
IntActiP37649, 1 interactor
STRINGi316385.ECDH10B_3706

Structurei

3D structure databases

ProteinModelPortaliP37649
SMRiP37649
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 236HAMPPROSITE-ProRule annotationAdd BLAST57
Domaini268 – 400GGDEFPROSITE-ProRule annotationAdd BLAST133
Domaini409 – 662EALPROSITE-ProRule annotationAdd BLAST254

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
ENOG410XNMH LUCA
HOGENOMiHOG000125924
PhylomeDBiP37649

Family and domain databases

CDDicd01948 EAL, 1 hit
Gene3Di3.20.20.450, 1 hit
InterProiView protein in InterPro
IPR001633 EAL_dom
IPR035919 EAL_sf
IPR033419 GAPES3
IPR000160 GGDEF_dom
IPR003660 HAMP_dom
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00563 EAL, 1 hit
PF17154 GAPES3, 1 hit
PF00990 GGDEF, 1 hit
PF00672 HAMP, 1 hit
SMARTiView protein in SMART
SM00052 EAL, 1 hit
SM00267 GGDEF, 1 hit
SUPFAMiSSF141868 SSF141868, 1 hit
SSF55073 SSF55073, 1 hit
PROSITEiView protein in PROSITE
PS50883 EAL, 1 hit
PS50887 GGDEF, 1 hit
PS50885 HAMP, 1 hit

Sequencei

Sequence statusi: Complete.

P37649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVSRSLTIK QMAMVAAVVL VFVFIFCTVL LFHLVQQNRY NTATQLESIA
60 70 80 90 100
RSVREPLSSA ILKGDIPEAE AILASIKPAG VVSRADVVLP NQFQALRKSF
110 120 130 140 150
IPERPVPVMV TRLFELPVQI SLGVYSLERP ANPQPIAYLV LQADSFRMYK
160 170 180 190 200
FVMSTLSTLV TIYLLLSLIL TVAISWCINR LILHPLRNIA RELNAIPAKE
210 220 230 240 250
LVGHQLALPR LHQDDEIGML VRSYNLNQQL LQRHYEEQNE NAMRFPVSDL
260 270 280 290 300
PNKALLMEML EQVVARKQTT ALMIITCETL RDTAGVLKEA QREILLLTLV
310 320 330 340 350
EKLKSVLSPR MILAQISGYD FAVIANGVQE PWHAITLGQQ VLTIMSERLP
360 370 380 390 400
IERIQLRPHC SIGVAMFYGD LTAEQLYSRA ISAAFTARHK GKNQIQFFDP
410 420 430 440 450
QQMEAAQKRL TEESDILNAL ENHQFAIWLQ PQVEMTSGKL VSAEVLLRIQ
460 470 480 490 500
QPDGSWDLPD GLIDRIECCG LMVTVGHWVL EESCRLLAAW QERGIMLPLS
510 520 530 540 550
VNLSALQLMH PNMVADMLEL LTRYRIQPGT LILEVTESRR IDDPHAAVAI
560 570 580 590 600
LRPLRNAGVR VALDDFGMGY AGLRQLQHMK SLPIDVLKID KMFVEGLPGD
610 620 630 640 650
SSMIAAIIML AQSLNLQMIA EGVETEAQRD WLAKAGVGIA QGFLFARPLP
660
IEIFEESYLE EK
Length:662
Mass (Da):74,381
Last modified:June 15, 2010 - v3
Checksum:i62CFA842B5851302
GO

Sequence cautioni

The sequence AAB18506 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE77765 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA Translation: AAB18506.1 Different initiation.
U00096 Genomic DNA Translation: AAC76554.4
AP009048 Genomic DNA Translation: BAE77765.1 Different initiation.
PIRiS47750
RefSeqiNP_417986.4, NC_000913.3
WP_001266293.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76554; AAC76554; b3529
BAE77765; BAE77765; BAE77765
GeneIDi948048
KEGGiecj:JW5943
eco:b3529
PATRICifig|511145.12.peg.3640

Similar proteinsi

Entry informationi

Entry nameiPDEK_ECOLI
AccessioniPrimary (citable) accession number: P37649
Secondary accession number(s): Q2M7J1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: June 15, 2010
Last modified: June 20, 2018
This is version 135 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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