P37647 (KDGK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxygluconokinase
EC=2.7.1.45

Alternative name(s):
2-keto-3-deoxygluconokinase
3-deoxy-2-oxo-D-gluconate kinase
KDG kinase

Gene names

Name:	kdgK
Synonyms:	yhjI
Ordered Locus Names:	b3526, JW5668

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-dehydro-3-deoxy-D-gluconate = ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate.
Sequence similarities	Belongs to the carbohydrate kinase PfkB family.
Sequence caution	The sequence AAB18503.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-galacturonate catabolic process Inferred from mutant phenotype PubMed 4359651PubMed 4944816. Source: EcoCyc D-glucuronate catabolic process Inferred from mutant phenotype PubMed 4359651PubMed 4944816. Source: EcoCyc response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki
Molecular_function	2-dehydro-3-deoxygluconokinase activity Inferred from direct assay PubMed 4944816. Source: EcoCyc ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	2-dehydro-3-deoxygluconokinase		PRO_0000080085

Sequences

	Sequence		Length	Mass (Da)
	P37647 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 53FCD14696BCFA33	FASTA	309	33,962
	10 20 30 40 50 60 MSKKIAVIGE CMIELSEKGA DVKRGFGGDT LNTSVYIARQ VDPAALTVHY VTALGTDSFS 70 80 90 100 110 120 QQMLDAWHGE NVDTSLTQRM ENRLPGLYYI ETDSTGERTF YYWRNEAAAK FWLESEQSAA 130 140 150 160 170 180 ICEELANFDY LYLSGISLAI LSPTSREKLL SLLRECRANG GKVIFDNNYR PRLWASKEET 190 200 210 220 230 240 QQVYQQMLEC TDIAFLTLDD EDALWGQQPV EDVIARTHNA GVKEVVVKRG ADSCLVSIAG 250 260 270 280 290 300 EGLVDVPAVK LPKEKVIDTT AAGDSFSAGY LAVRLTGGSA EDAAKRGHLT ASTVIQYRGA IIPREAMPA

References

[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00039 Genomic DNA. Translation: AAB18503.1. Different initiation. U00096 Genomic DNA. Translation: AAC76551.2. AP009048 Genomic DNA. Translation: BAE77768.1.
PIR	S47747.
RefSeq	NP_417983.2. NC_000913.2. YP_491909.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P37647.
SMR	P37647. Positions 2-308.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10056N.
IntAct	P37647. 2 interactions.
MINT	MINT-1219186.
STRING	511145.b3526.
Proteomic databases
PaxDb	P37647.
PRIDE	P37647.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76551; AAC76551; b3526. BAE77768; BAE77768; BAE77768.
GeneID	12933534. 948041.
KEGG	ecj:Y75_p3651. eco:b3526.
PATRIC	32122516. VBIEscCol129921_3636.
Organism-specific databases
EchoBASE	EB2163.
EcoGene	EG12253. kdgK.
Phylogenomic databases
eggNOG	COG0524.
HOGENOM	HOG000235952.
KO	K00874.
OMA	NRGFGGD.
ProtClustDB	CLSK880706.
Enzyme and pathway databases
BioCyc	EcoCyc:DEOXYGLUCONOKIN-MONOMER. ECOL316407:JW5668-MONOMER. MetaCyc:DEOXYGLUCONOKIN-MONOMER.
Gene expression databases
Genevestigator	P37647.
Family and domain databases
InterPro	IPR002173. Carboh/pur_kinase_PfkB_CS. IPR011611. PfkB_dom. [Graphical view]
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PROSITE	PS00583. PFKB_KINASES_1. False negative. PS00584. PFKB_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDGK_ECOLI

Accession

Primary (citable) accession number: P37647
Secondary accession number(s): P78117, Q2M7I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program