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Protein

Cyclic di-GMP phosphodiesterase YhjH

Gene

yhjH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

A bis-(3'-5') cyclic diguanylic acid (c-di-GMP) phosphodiesterase involved in regulating the levels of c-di-GMP that control cell motility (the flagella) and adhesion (adhesive curli fimbriae). The YhjH effect on flagella is controlled via the c-di-GMP-binding flagellar brake protein YcgR, however curli expression is not regulated via YcgR. Forms a network with different diguanylate cyclases (DosC, YeaJ, YedQ, YegE have been identified) to regulate c-di-GMP levels. Flagellar activity is high at low c-di-GMP levels whereas curli fimbria are induced at high c-di-GMP levels. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.1 Publication

Catalytic activityi

Cyclic di-3',5'-guanylate + H2O = 5'-phosphoguanylyl(3'->5')guanosine.1 Publication

GO - Molecular functioni

  • cyclic-guanylate-specific phosphodiesterase activity Source: EcoCyc
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • cell motility Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

c-di-GMP, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12252-MONOMER.
ECOL316407:JW3493-MONOMER.
MetaCyc:EG12252-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic di-GMP phosphodiesterase YhjH (EC:3.1.4.52)
Gene namesi
Name:yhjH
Ordered Locus Names:b3525, JW3493
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12252. yhjH.

Pathology & Biotechi

Disruption phenotypei

Disruption leads to increased expression of adhesive curli fimbriae genes (PubMed:18765794), including CsgD (PubMed:19332833). Also leads to a severe reduction in swarm size and swimming velocity, and 80% reduced concentrations of c-di-GMP. Disruption of ycgR, or concomitant disruption of dosC, yegE, yedQ and yfiN completely restores motility, suggesting these 4 genes, together with this c-di-GMP phosphodiesterase, form a network that regulates cell motility by altering levels of c-di-GMP (PubMed:20303158). Overlapping but different results were seen by another group, where disruption of yeaJ, another probable diguanylate cyclase, partially suppresses the yhjH disruption, full suppression requires concomitant disruption of yeaJ, yedQ and yegE (PubMed:18765794).4 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi48 – 481E → A: Loss of c-di-GMP phosphodiesterase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255Cyclic di-GMP phosphodiesterase YhjHPRO_0000169573Add
BLAST

Proteomic databases

PaxDbiP37646.

Expressioni

Inductioni

A class 3 flagellar gene, it is dependent on FliA for expression. Induced in early post-exponential phase at both 28 and 37 degrees Celsius, it shuts down later than class 1 and class 2 operon genes. Expression has to be shut down for adhesive curli fimbriae to be induced, i.e. on solid medium where biofilms form. Repressed by RpoS.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4262527. 12 interactions.
IntActiP37646. 2 interactions.
STRINGi511145.b3525.

Structurei

3D structure databases

ProteinModelPortaliP37646.
SMRiP37646. Positions 36-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 255243EALPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 EAL domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CMB. Bacteria.
ENOG410Z0RI. LUCA.
HOGENOMiHOG000125902.
InParanoidiP37646.
OMAiARDLFIM.
PhylomeDBiP37646.

Family and domain databases

CDDicd01948. EAL. 1 hit.
Gene3Di3.20.20.450. 1 hit.
InterProiIPR001633. EAL_dom.
[Graphical view]
PfamiPF00563. EAL. 1 hit.
[Graphical view]
SMARTiSM00052. EAL. 1 hit.
[Graphical view]
SUPFAMiSSF141868. SSF141868. 1 hit.
PROSITEiPS50883. EAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRQVIQRIS NPEASIESLQ ERRFWLQCER AYTWQPIYQT CGRLMAVELL
60 70 80 90 100
TVVTHPLNPS QRLPPDRYFT EITVSHRMEV VKEQIDLLAQ KADFFIEHGL
110 120 130 140 150
LASVNIDGPT LIALRQQPKI LRQIERLPWL RFELVEHIRL PKDSTFASMC
160 170 180 190 200
EFGPLWLDDF GTGMANFSAL SEVRYDYIKI ARELFVMLRQ SPEGRTLFSQ
210 220 230 240 250
LLHLMNRYCR GVIVEGVETP EEWRDVQNSP AFAAQGWFLS RPAPIETLNT

AVLAL
Length:255
Mass (Da):29,601
Last modified:December 15, 1998 - v3
Checksum:iF91C1893C8F1B508
GO

Sequence cautioni

The sequence AAB18502 differs from that shown. Reason: Frameshift at position 216. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18502.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76550.2.
AP009048 Genomic DNA. Translation: BAE77769.1.
PIRiH65150.
RefSeqiNP_417982.2. NC_000913.3.
WP_001295219.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76550; AAC76550; b3525.
BAE77769; BAE77769; BAE77769.
GeneIDi948042.
KEGGiecj:JW3493.
eco:b3525.
PATRICi32122512. VBIEscCol129921_3634.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18502.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76550.2.
AP009048 Genomic DNA. Translation: BAE77769.1.
PIRiH65150.
RefSeqiNP_417982.2. NC_000913.3.
WP_001295219.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37646.
SMRiP37646. Positions 36-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262527. 12 interactions.
IntActiP37646. 2 interactions.
STRINGi511145.b3525.

Proteomic databases

PaxDbiP37646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76550; AAC76550; b3525.
BAE77769; BAE77769; BAE77769.
GeneIDi948042.
KEGGiecj:JW3493.
eco:b3525.
PATRICi32122512. VBIEscCol129921_3634.

Organism-specific databases

EchoBASEiEB2162.
EcoGeneiEG12252. yhjH.

Phylogenomic databases

eggNOGiENOG4105CMB. Bacteria.
ENOG410Z0RI. LUCA.
HOGENOMiHOG000125902.
InParanoidiP37646.
OMAiARDLFIM.
PhylomeDBiP37646.

Enzyme and pathway databases

BioCyciEcoCyc:EG12252-MONOMER.
ECOL316407:JW3493-MONOMER.
MetaCyc:EG12252-MONOMER.

Miscellaneous databases

PROiP37646.

Family and domain databases

CDDicd01948. EAL. 1 hit.
Gene3Di3.20.20.450. 1 hit.
InterProiIPR001633. EAL_dom.
[Graphical view]
PfamiPF00563. EAL. 1 hit.
[Graphical view]
SMARTiSM00052. EAL. 1 hit.
[Graphical view]
SUPFAMiSSF141868. SSF141868. 1 hit.
PROSITEiPS50883. EAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYHJH_ECOLI
AccessioniPrimary (citable) accession number: P37646
Secondary accession number(s): Q2M7I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 15, 1998
Last modified: September 7, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.