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Protein

Cyclic di-GMP phosphodiesterase YhjH

Gene

yhjH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

A bis-(3'-5') cyclic diguanylic acid (c-di-GMP) phosphodiesterase involved in regulating the levels of c-di-GMP that control cell motility (the flagella) and adhesion (adhesive curli fimbriae). The YhjH effect on flagella is controlled via the c-di-GMP-binding flagellar brake protein YcgR, however curli expression is not regulated via YcgR. Forms a network with different diguanylate cyclases (DosC, YeaJ, YedQ, YegE have been identified) to regulate c-di-GMP levels. Flagellar activity is high at low c-di-GMP levels whereas curli fimbria are induced at high c-di-GMP levels. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.1 Publication

Catalytic activityi

Cyclic di-3',5'-guanylate + H2O = 5'-phosphoguanylyl(3'->5')guanosine.1 Publication

GO - Molecular functioni

  • cyclic-guanylate-specific phosphodiesterase activity Source: EcoCyc
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • regulation of bacterial-type flagellum-dependent cell motility Source: EcoCyc

Keywordsi

Molecular functionHydrolase
Ligandc-di-GMP, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12252-MONOMER.
MetaCyc:EG12252-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic di-GMP phosphodiesterase YhjH (EC:3.1.4.52)
Gene namesi
Name:yhjH
Ordered Locus Names:b3525, JW3493
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12252. yhjH.

Pathology & Biotechi

Disruption phenotypei

Disruption leads to increased expression of adhesive curli fimbriae genes (PubMed:18765794), including CsgD (PubMed:19332833). Also leads to a severe reduction in swarm size and swimming velocity, and 80% reduced concentrations of c-di-GMP. Disruption of ycgR, or concomitant disruption of dosC, yegE, yedQ and yfiN completely restores motility, suggesting these 4 genes, together with this c-di-GMP phosphodiesterase, form a network that regulates cell motility by altering levels of c-di-GMP (PubMed:20303158). Overlapping but different results were seen by another group, where disruption of yeaJ, another probable diguanylate cyclase, partially suppresses the yhjH disruption, full suppression requires concomitant disruption of yeaJ, yedQ and yegE (PubMed:18765794).4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi48E → A: Loss of c-di-GMP phosphodiesterase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001695731 – 255Cyclic di-GMP phosphodiesterase YhjHAdd BLAST255

Proteomic databases

PaxDbiP37646.
PRIDEiP37646.

Expressioni

Inductioni

A class 3 flagellar gene, it is dependent on FliA for expression. Induced in early post-exponential phase at both 28 and 37 degrees Celsius, it shuts down later than class 1 and class 2 operon genes. Expression has to be shut down for adhesive curli fimbriae to be induced, i.e. on solid medium where biofilms form. Repressed by RpoS.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4262527. 12 interactors.
IntActiP37646. 2 interactors.
STRINGi316385.ECDH10B_3702.

Structurei

3D structure databases

ProteinModelPortaliP37646.
SMRiP37646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 255EALPROSITE-ProRule annotationAdd BLAST243

Phylogenomic databases

eggNOGiENOG4105CMB. Bacteria.
ENOG410Z0RI. LUCA.
HOGENOMiHOG000125902.
InParanoidiP37646.
KOiK21086.
PhylomeDBiP37646.

Family and domain databases

CDDicd01948. EAL. 1 hit.
Gene3Di3.20.20.450. 1 hit.
InterProiView protein in InterPro
IPR001633. EAL_dom.
PfamiView protein in Pfam
PF00563. EAL. 1 hit.
SMARTiView protein in SMART
SM00052. EAL. 1 hit.
SUPFAMiSSF141868. SSF141868. 1 hit.
PROSITEiView protein in PROSITE
PS50883. EAL. 1 hit.

Sequencei

Sequence statusi: Complete.

P37646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRQVIQRIS NPEASIESLQ ERRFWLQCER AYTWQPIYQT CGRLMAVELL
60 70 80 90 100
TVVTHPLNPS QRLPPDRYFT EITVSHRMEV VKEQIDLLAQ KADFFIEHGL
110 120 130 140 150
LASVNIDGPT LIALRQQPKI LRQIERLPWL RFELVEHIRL PKDSTFASMC
160 170 180 190 200
EFGPLWLDDF GTGMANFSAL SEVRYDYIKI ARELFVMLRQ SPEGRTLFSQ
210 220 230 240 250
LLHLMNRYCR GVIVEGVETP EEWRDVQNSP AFAAQGWFLS RPAPIETLNT

AVLAL
Length:255
Mass (Da):29,601
Last modified:December 15, 1998 - v3
Checksum:iF91C1893C8F1B508
GO

Sequence cautioni

The sequence AAB18502 differs from that shown. Reason: Frameshift at position 216.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18502.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76550.2.
AP009048 Genomic DNA. Translation: BAE77769.1.
PIRiH65150.
RefSeqiNP_417982.2. NC_000913.3.
WP_001295219.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76550; AAC76550; b3525.
BAE77769; BAE77769; BAE77769.
GeneIDi948042.
KEGGiecj:JW3493.
eco:b3525.
PATRICifig|511145.12.peg.3634.

Similar proteinsi

Entry informationi

Entry nameiYHJH_ECOLI
AccessioniPrimary (citable) accession number: P37646
Secondary accession number(s): Q2M7I7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 15, 1998
Last modified: August 30, 2017
This is version 118 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene