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Protein

HTH-type transcriptional regulator GadX

Gene

gadX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Positively regulates the expression of about fifteen genes involved in acid resistance such as gadA, gadB and gadC. Depending on the conditions (growth phase and medium), can repress gadW.6 Publications

Miscellaneous

GadX and GadW are part of a complex system required for acid resistance via the regulation of gadA and gadBC. Many of the described circuits use mutants missing one of the network components. In the wild-type situation, however, many of the scenarios are not easily observed.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi161 – 180H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG12243-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator GadX
Gene namesi
Name:gadX
Synonyms:yhiX
Ordered Locus Names:b3516, JW3484
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12243. gadX.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001945181 – 274HTH-type transcriptional regulator GadXAdd BLAST274

Proteomic databases

PaxDbiP37639.
PRIDEiP37639.

Expressioni

Inductioni

Expression can be activated by RpoS and repressed by CRP, H-NS and GadW, depending on the conditions.3 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4262524. 14 interactors.
IntActiP37639. 6 interactors.
STRINGi316385.ECDH10B_3693.

Structurei

Secondary structure

1274
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi143 – 152Combined sources10
Helixi161 – 167Combined sources7
Helixi172 – 181Combined sources10
Helixi186 – 202Combined sources17
Helixi209 – 215Combined sources7
Helixi221 – 232Combined sources12
Helixi236 – 242Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MKLX-ray2.15A/B137-255[»]
ProteinModelPortaliP37639.
SMRiP37639.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108NKV. Bacteria.
ENOG410XRJG. LUCA.
HOGENOMiHOG000125870.
InParanoidiP37639.
KOiK21905.
PhylomeDBiP37639.

Family and domain databases

InterProiView protein in InterPro
IPR009057. Homeobox-like_sf.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR020449. Tscrpt_reg_HTH_AraC-type.
PfamiView protein in Pfam
PF12833. HTH_18. 1 hit.
PRINTSiPR00032. HTHARAC.
SMARTiView protein in SMART
SM00342. HTH_ARAC. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiView protein in PROSITE
PS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P37639-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSLHGNCLI AYARHKYILT MVNGEYRYFN GGDLVFADAS QIRVDKCVEN
60 70 80 90 100
FVFVSRDTLS LFLPMLKEEA LNLHAHKKVS SLLVHHCSRD IPVFQEVAQL
110 120 130 140 150
SQNKNLRYAE MLRKRALIFA LLSVFLEDEH FIPLLLNVLQ PNMRTRVCTV
160 170 180 190 200
INNNIAHEWT LARIASELLM SPSLLKKKLR EEETSYSQLL TECRMQRALQ
210 220 230 240 250
LIVIHGFSIK RVAVSCGYHS VSYFIYVFRN YYGMTPTEYQ ERSAQRLSNR
260 270
DSAASIVAQG NFYGTDRSAE GIRL
Length:274
Mass (Da):31,563
Last modified:October 1, 1994 - v1
Checksum:iC5B742F296436DC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18492.1.
U00096 Genomic DNA. Translation: AAC76541.1.
AP009048 Genomic DNA. Translation: BAE77778.1.
PIRiS47736.
RefSeqiNP_417973.1. NC_000913.3.
WP_001191059.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76541; AAC76541; b3516.
BAE77778; BAE77778; BAE77778.
GeneIDi948028.
KEGGiecj:JW3484.
eco:b3516.
PATRICifig|1411691.4.peg.3202.

Similar proteinsi

Entry informationi

Entry nameiGADX_ECOLI
AccessioniPrimary (citable) accession number: P37639
Secondary accession number(s): Q2M7H8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 22, 2017
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references