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Protein

HTH-type transcriptional regulator GadX

Gene

gadX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Positively regulates the expression of about fifteen genes involved in acid resistance such as gadA, gadB and gadC. Depending on the conditions (growth phase and medium), can repress gadW.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi161 – 180H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12243-MONOMER.
ECOL316407:JW3484-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator GadX
Gene namesi
Name:gadX
Synonyms:yhiX
Ordered Locus Names:b3516, JW3484
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12243. gadX.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001945181 – 274HTH-type transcriptional regulator GadXAdd BLAST274

Proteomic databases

PaxDbiP37639.
PRIDEiP37639.

Expressioni

Inductioni

Expression can be activated by RpoS and repressed by CRP, H-NS and GadW, depending on the conditions.3 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4262524. 14 interactors.
IntActiP37639. 6 interactors.
STRINGi511145.b3516.

Structurei

3D structure databases

ProteinModelPortaliP37639.
SMRiP37639.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108NKV. Bacteria.
ENOG410XRJG. LUCA.
HOGENOMiHOG000125870.
InParanoidiP37639.
OMAiNCGPAAS.
PhylomeDBiP37639.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37639-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSLHGNCLI AYARHKYILT MVNGEYRYFN GGDLVFADAS QIRVDKCVEN
60 70 80 90 100
FVFVSRDTLS LFLPMLKEEA LNLHAHKKVS SLLVHHCSRD IPVFQEVAQL
110 120 130 140 150
SQNKNLRYAE MLRKRALIFA LLSVFLEDEH FIPLLLNVLQ PNMRTRVCTV
160 170 180 190 200
INNNIAHEWT LARIASELLM SPSLLKKKLR EEETSYSQLL TECRMQRALQ
210 220 230 240 250
LIVIHGFSIK RVAVSCGYHS VSYFIYVFRN YYGMTPTEYQ ERSAQRLSNR
260 270
DSAASIVAQG NFYGTDRSAE GIRL
Length:274
Mass (Da):31,563
Last modified:October 1, 1994 - v1
Checksum:iC5B742F296436DC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18492.1.
U00096 Genomic DNA. Translation: AAC76541.1.
AP009048 Genomic DNA. Translation: BAE77778.1.
PIRiS47736.
RefSeqiNP_417973.1. NC_000913.3.
WP_001191059.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76541; AAC76541; b3516.
BAE77778; BAE77778; BAE77778.
GeneIDi948028.
KEGGiecj:JW3484.
eco:b3516.
PATRICi32122494. VBIEscCol129921_3625.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18492.1.
U00096 Genomic DNA. Translation: AAC76541.1.
AP009048 Genomic DNA. Translation: BAE77778.1.
PIRiS47736.
RefSeqiNP_417973.1. NC_000913.3.
WP_001191059.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37639.
SMRiP37639.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262524. 14 interactors.
IntActiP37639. 6 interactors.
STRINGi511145.b3516.

Proteomic databases

PaxDbiP37639.
PRIDEiP37639.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76541; AAC76541; b3516.
BAE77778; BAE77778; BAE77778.
GeneIDi948028.
KEGGiecj:JW3484.
eco:b3516.
PATRICi32122494. VBIEscCol129921_3625.

Organism-specific databases

EchoBASEiEB2154.
EcoGeneiEG12243. gadX.

Phylogenomic databases

eggNOGiENOG4108NKV. Bacteria.
ENOG410XRJG. LUCA.
HOGENOMiHOG000125870.
InParanoidiP37639.
OMAiNCGPAAS.
PhylomeDBiP37639.

Enzyme and pathway databases

BioCyciEcoCyc:EG12243-MONOMER.
ECOL316407:JW3484-MONOMER.

Miscellaneous databases

PROiP37639.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGADX_ECOLI
AccessioniPrimary (citable) accession number: P37639
Secondary accession number(s): Q2M7H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

GadX and GadW are part of a complex system required for acid resistance via the regulation of gadA and gadBC. Many of the described circuits use mutants missing one of the network components. In the wild-type situation, however, many of the scenarios are not easily observed.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.