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Protein

Multidrug resistance protein MdtF

Gene

mdtF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the tripartite efflux system MdtEF-TolC, which confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium.1 Publication

GO - Molecular functioni

  • transporter activity Source: EcoliWiki

GO - Biological processi

  • drug transmembrane transport Source: EcoliWiki
  • response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YHIV-MONOMER.
ECOL316407:JW3482-MONOMER.

Protein family/group databases

TCDBi2.A.6.2.13. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein MdtF
Gene namesi
Name:mdtF
Synonyms:yhiV
Ordered Locus Names:b3514, JW3482
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12241. mdtF.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicBy similarity9
Transmembranei10 – 28Helical; Name=1By similarityAdd BLAST19
Topological domaini29 – 339PeriplasmicBy similarityAdd BLAST311
Transmembranei340 – 359Helical; Name=2By similarityAdd BLAST20
Topological domaini360 – 365CytoplasmicBy similarity6
Transmembranei366 – 385Helical; Name=3By similarityAdd BLAST20
Topological domaini386 – 391PeriplasmicBy similarity6
Transmembranei392 – 413Helical; Name=4By similarityAdd BLAST22
Topological domaini414 – 441CytoplasmicBy similarityAdd BLAST28
Transmembranei442 – 460Helical; Name=5By similarityAdd BLAST19
Topological domaini461 – 473PeriplasmicBy similarityAdd BLAST13
Transmembranei474 – 496Helical; Name=6By similarityAdd BLAST23
Topological domaini497 – 536CytoplasmicBy similarityAdd BLAST40
Transmembranei537 – 555Helical; Name=7By similarityAdd BLAST19
Topological domaini556 – 870PeriplasmicBy similarityAdd BLAST315
Transmembranei871 – 890Helical; Name=8By similarityAdd BLAST20
Topological domaini891 – 896CytoplasmicBy similarity6
Transmembranei897 – 916Helical; Name=9By similarityAdd BLAST20
Topological domaini917 – 922PeriplasmicBy similarity6
Transmembranei923 – 944Helical; Name=10By similarityAdd BLAST22
Topological domaini945 – 972CytoplasmicBy similarityAdd BLAST28
Transmembranei973 – 991Helical; Name=11By similarityAdd BLAST19
Topological domaini992 – 1004PeriplasmicBy similarityAdd BLAST13
Transmembranei1005 – 1027Helical; Name=12By similarityAdd BLAST23
Topological domaini1028 – 1037CytoplasmicBy similarity10

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001618411 – 1037Multidrug resistance protein MdtFAdd BLAST1037

Proteomic databases

PaxDbiP37637.
PRIDEiP37637.

Expressioni

Inductioni

Induced by EvgA, probably via YdeO.4 Publications

Interactioni

Subunit structurei

Homotrimer. Part of the tripartite efflux system MdtEF-TolC, which is composed of an inner membrane transporter, MdtF, a membrane fusion protein, MdtE, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes.2 Publications

Protein-protein interaction databases

BioGridi4262521. 264 interactors.
IntActiP37637. 1 interactor.
STRINGi511145.b3514.

Structurei

3D structure databases

ProteinModelPortaliP37637.
SMRiP37637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZS. Bacteria.
COG0841. LUCA.
HOGENOMiHOG000158129.
InParanoidiP37637.
KOiK18899.
OMAiKNASGEM.
PhylomeDBiP37637.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
TIGRFAMsiTIGR00915. 2A0602. 1 hit.

Sequencei

Sequence statusi: Complete.

P37637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANYFIDRPV FAWVLAIIMM LAGGLAIMNL PVAQYPQIAP PTITVSATYP
60 70 80 90 100
GADAQTVEDS VTQVIEQNMN GLDGLMYMSS TSDAAGNASI TLTFETGTSP
110 120 130 140 150
DIAQVQVQNK LQLAMPSLPE AVQQQGISVD KSSSNILMVA AFISDNGSLN
160 170 180 190 200
QYDIADYVAS NIKDPLSRTA GVGSVQLFGS EYAMRIWLDP QKLNKYNLVP
210 220 230 240 250
SDVISQIKVQ NNQISGGQLG GMPQAADQQL NASIIVQTRL QTPEEFGKIL
260 270 280 290 300
LKVQQDGSQV LLRDVARVEL GAEDYSTVAR YNGKPAAGIA IKLAAGANAL
310 320 330 340 350
DTSRAVKEEL NRLSAYFPAS LKTVYPYDTT PFIEISIQEV FKTLVEAIIL
360 370 380 390 400
VFLVMYLFLQ NFRATIIPTI AVPVVILGTF AILSAVGFTI NTLTMFGMVL
410 420 430 440 450
AIGLLVDDAI VVVENVERVI AEDKLPPKEA THKSMGQIQR ALVGIAVVLS
460 470 480 490 500
AVFMPMAFMS GATGEIYRQF SITLISSMLL SVFVAMSLTP ALCATILKAA
510 520 530 540 550
PEGGHKPNAL FARFNTLFEK STQHYTDSTR SLLRCTGRYM VVYLLICAGM
560 570 580 590 600
AVLFLRTPTS FLPEEDQGVF MTTAQLPSGA TMVNTTKVLQ QVTDYYLTKE
610 620 630 640 650
KDNVQSVFTV GGFGFSGQGQ NNGLAFISLK PWSERVGEEN SVTAIIQRAM
660 670 680 690 700
IALSSINKAV VFPFNLPAVA ELGTASGFDM ELLDNGNLGH EKLTQARNEL
710 720 730 740 750
LSLAAQSPNQ VTGVRPNGLE DTPMFKVNVN AAKAEAMGVA LSDINQTIST
760 770 780 790 800
AFGSSYVNDF LNQGRVKKVY VQAGTPFRML PDNINQWYVR NASGTMAPLS
810 820 830 840 850
AYSSTEWTYG SPRLERYNGI PSMEILGEAA AGKSTGDAMK FMADLVAKLP
860 870 880 890 900
AGVGYSWTGL SYQEALSSNQ APALYAISLV VVFLALAALY ESWSIPFSVM
910 920 930 940 950
LVVPLGVVGA LLATDLRGLS NDVYFQVGLL TTIGLSAKNA ILIVEFAVEM
960 970 980 990 1000
MQKEGKTPIE AIIEAARMRL RPILMTSLAF ILGVLPLVIS HGAGSGAQNA
1010 1020 1030
VGTGVMGGMF AATVLAIYFV PVFFVVVEHL FARFKKA
Length:1,037
Mass (Da):111,517
Last modified:October 1, 1994 - v1
Checksum:i3B67502160F51724
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18490.1.
U00096 Genomic DNA. Translation: AAC76539.1.
AP009048 Genomic DNA. Translation: BAE77780.1.
PIRiS47734.
RefSeqiNP_417971.1. NC_000913.3.
WP_000024872.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76539; AAC76539; b3514.
BAE77780; BAE77780; BAE77780.
GeneIDi948030.
KEGGiecj:JW3482.
eco:b3514.
PATRICi32122488. VBIEscCol129921_3622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18490.1.
U00096 Genomic DNA. Translation: AAC76539.1.
AP009048 Genomic DNA. Translation: BAE77780.1.
PIRiS47734.
RefSeqiNP_417971.1. NC_000913.3.
WP_000024872.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37637.
SMRiP37637.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262521. 264 interactors.
IntActiP37637. 1 interactor.
STRINGi511145.b3514.

Protein family/group databases

TCDBi2.A.6.2.13. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

PaxDbiP37637.
PRIDEiP37637.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76539; AAC76539; b3514.
BAE77780; BAE77780; BAE77780.
GeneIDi948030.
KEGGiecj:JW3482.
eco:b3514.
PATRICi32122488. VBIEscCol129921_3622.

Organism-specific databases

EchoBASEiEB2152.
EcoGeneiEG12241. mdtF.

Phylogenomic databases

eggNOGiENOG4105BZS. Bacteria.
COG0841. LUCA.
HOGENOMiHOG000158129.
InParanoidiP37637.
KOiK18899.
OMAiKNASGEM.
PhylomeDBiP37637.

Enzyme and pathway databases

BioCyciEcoCyc:YHIV-MONOMER.
ECOL316407:JW3482-MONOMER.

Miscellaneous databases

PROiP37637.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
TIGRFAMsiTIGR00915. 2A0602. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDTF_ECOLI
AccessioniPrimary (citable) accession number: P37637
Secondary accession number(s): Q2M7H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.