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Protein

Multidrug resistance protein MdtE

Gene

mdtE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the tripartite efflux system MdtEF-TolC, which confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG12240-MONOMER.
ECOL316407:JW3481-MONOMER.
RETL1328306-WGS:GSTH-3470-MONOMER.

Protein family/group databases

TCDBi8.A.1.6.3. the membrane fusion protein (mfp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein MdtE
Gene namesi
Name:mdtE
Synonyms:yhiU
Ordered Locus Names:b3513, JW3481
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12240. mdtE.

Subcellular locationi

  • Cell inner membrane Curated; Lipid-anchor PROSITE-ProRule annotation

GO - Cellular componenti

  • intrinsic component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020PROSITE-ProRule annotationAdd
BLAST
Chaini21 – 385365Multidrug resistance protein MdtEPRO_0000018708Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi21 – 211N-palmitoyl cysteinePROSITE-ProRule annotation
Lipidationi21 – 211S-diacylglycerol cysteinePROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP37636.
PaxDbiP37636.
PRIDEiP37636.

Expressioni

Inductioni

Induced by EvgA, probably via YdeO.4 Publications

Interactioni

Subunit structurei

Homotrimer. Part of the tripartite efflux system MdtEF-TolC, which is composed of an inner membrane transporter, MdtF, a membrane fusion protein, MdtE, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes.2 Publications

Protein-protein interaction databases

BioGridi4262520. 212 interactions.
IntActiP37636. 4 interactions.
STRINGi511145.b3513.

Structurei

3D structure databases

ProteinModelPortaliP37636.
SMRiP37636. Positions 34-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105C1P. Bacteria.
ENOG410XNVN. LUCA.
HOGENOMiHOG000158247.
InParanoidiP37636.
KOiK18898.
OMAiLQFAGVI.
OrthoDBiEOG6GJBSS.
PhylomeDBiP37636.

Family and domain databases

InterProiIPR032317. HlyD_D23.
IPR006143. RND_pump_MFP.
[Graphical view]
PfamiPF00529. HlyD. 1 hit.
PF16576. HlyD_D23. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01730. RND_mfp. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRRKLLIP LLFCGAMLTA CDDKSAENAA AMTPEVGVVT LSPGSVNVLS
60 70 80 90 100
ELPGRTVPYE VAEIRPQVGG IIIKRNFIEG DKVNQGDSLY QIDPAPLQAE
110 120 130 140 150
LNSAKGSLAK ALSTASNARI TFNRQASLLK TNYVSRQDYD TARTQLNEAE
160 170 180 190 200
ANVTVAKAAV EQATINLQYA NVTSPITGVS GKSSVTVGAL VTANQADSLV
210 220 230 240 250
TVQRLDPIYV DLTQSVQDFL RMKEEVASGQ IKQVQGSTPV QLNLENGKRY
260 270 280 290 300
SQTGTLKFSD PTVDETTGSV TLRAIFPNPN GDLLPGMYVT ALVDEGSRQN
310 320 330 340 350
VLLVPQEGVT HNAQGKATAL ILDKDDVVQL REIEASKAIG DQWVVTSGLQ
360 370 380
AGDRVIVSGL QRIRPGIKAR AISSSQENAS TESKQ
Length:385
Mass (Da):41,191
Last modified:October 1, 1994 - v1
Checksum:i2C825B6CDE15C70F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18489.1.
U00096 Genomic DNA. Translation: AAC76538.1.
AP009048 Genomic DNA. Translation: BAE77781.1.
PIRiS47733.
RefSeqiNP_417970.1. NC_000913.3.
WP_001081984.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76538; AAC76538; b3513.
BAE77781; BAE77781; BAE77781.
GeneIDi948031.
KEGGiecj:JW3481.
eco:b3513.
PATRICi32122486. VBIEscCol129921_3621.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18489.1.
U00096 Genomic DNA. Translation: AAC76538.1.
AP009048 Genomic DNA. Translation: BAE77781.1.
PIRiS47733.
RefSeqiNP_417970.1. NC_000913.3.
WP_001081984.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37636.
SMRiP37636. Positions 34-372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262520. 212 interactions.
IntActiP37636. 4 interactions.
STRINGi511145.b3513.

Protein family/group databases

TCDBi8.A.1.6.3. the membrane fusion protein (mfp) family.

Proteomic databases

EPDiP37636.
PaxDbiP37636.
PRIDEiP37636.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76538; AAC76538; b3513.
BAE77781; BAE77781; BAE77781.
GeneIDi948031.
KEGGiecj:JW3481.
eco:b3513.
PATRICi32122486. VBIEscCol129921_3621.

Organism-specific databases

EchoBASEiEB2151.
EcoGeneiEG12240. mdtE.

Phylogenomic databases

eggNOGiENOG4105C1P. Bacteria.
ENOG410XNVN. LUCA.
HOGENOMiHOG000158247.
InParanoidiP37636.
KOiK18898.
OMAiLQFAGVI.
OrthoDBiEOG6GJBSS.
PhylomeDBiP37636.

Enzyme and pathway databases

BioCyciEcoCyc:EG12240-MONOMER.
ECOL316407:JW3481-MONOMER.
RETL1328306-WGS:GSTH-3470-MONOMER.

Miscellaneous databases

PROiP37636.

Family and domain databases

InterProiIPR032317. HlyD_D23.
IPR006143. RND_pump_MFP.
[Graphical view]
PfamiPF00529. HlyD. 1 hit.
PF16576. HlyD_D23. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01730. RND_mfp. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Analysis of a complete library of putative drug transporter genes in Escherichia coli."
    Nishino K., Yamaguchi A.
    J. Bacteriol. 183:5803-5812(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MULTIDRUG RESISTANCE.
  5. "EvgA of the two-component signal transduction system modulates production of the yhiUV multidrug transporter in Escherichia coli."
    Nishino K., Yamaguchi A.
    J. Bacteriol. 184:2319-2323(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, SUBUNIT.
  6. "Escherichia coli gene expression responsive to levels of the response regulator EvgA."
    Masuda N., Church G.M.
    J. Bacteriol. 184:6225-6234(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli."
    Eguchi Y., Oshima T., Mori H., Aono R., Yamamoto K., Ishihama A., Utsumi R.
    Microbiology 149:2819-2828(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "Regulatory network of acid resistance genes in Escherichia coli."
    Masuda N., Church G.M.
    Mol. Microbiol. 48:699-712(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  9. Cited for: SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BL21-DE3.

Entry informationi

Entry nameiMDTE_ECOLI
AccessioniPrimary (citable) accession number: P37636
Secondary accession number(s): Q2M7H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: March 16, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.