Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosome-associated ATPase

Gene

rbbA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits an intrinsic ATPase activity that is stimulated by both 70S ribosomes and 30S ribosomal subunits. Could be involved in protein-chain elongation and in release of deacyl-tRNA from ribosomes after peptide bond synthesis. Stimulates the synthesis of polyphenylalanine in vitro.3 Publications

Enzyme regulationi

Hygromycin B inhibits RbbA ATPase activity and releases RbbA from 70S ribosomes.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi309 – 316ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: EcoCyc
  • ATPase activity, coupled Source: EcoCyc
  • ATP binding Source: UniProtKB-KW
  • ribosomal small subunit binding Source: EcoCyc

GO - Biological processi

  • positive regulation of translation Source: EcoCyc
  • transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YHIH-MONOMER.
ECOL316407:JW5676-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-associated ATPaseCurated
Alternative name(s):
Ribosomal bound ATPase1 Publication
Gene namesi
Name:rbbA1 Publication
Synonyms:yhiG, yhiH
Ordered Locus Names:b3486, JW5676
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12223. rbbA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei563 – 583HelicalSequence analysisAdd BLAST21
Transmembranei716 – 736HelicalSequence analysisAdd BLAST21
Transmembranei769 – 789HelicalSequence analysisAdd BLAST21
Transmembranei796 – 816HelicalSequence analysisAdd BLAST21
Transmembranei824 – 844HelicalSequence analysisAdd BLAST21
Transmembranei886 – 906HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cell pole Source: EcoCyc
  • cytosolic small ribosomal subunit Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion mutant is slightly elongated at both 15 and 32 degrees Celsius and displays a significant loss of translation fidelity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000931901 – 911Ribosome-associated ATPaseAdd BLAST911

Proteomic databases

PaxDbiP37624.
PRIDEiP37624.

Interactioni

Subunit structurei

Interacts with both 70S ribosomes and 30S ribosomal subunits, and with 16S RNA (PubMed:10607404, PubMed:11168361, PubMed:16495476). Binds EF-Tu (PubMed:11168361). Co-purifies with the inner membrane protein YhjD (PubMed:21556145).4 Publications

Protein-protein interaction databases

BioGridi4259649. 229 interactors.
DIPiDIP-12363N.
IntActiP37624. 1 interactor.
STRINGi511145.b3486.

Structurei

3D structure databases

ProteinModelPortaliP37624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 248ABC transporter 1PROSITE-ProRule annotationAdd BLAST236
Domaini277 – 507ABC transporter 2PROSITE-ProRule annotationAdd BLAST231
Domaini683 – 909ABC transmembrane type-2PROSITE-ProRule annotationAdd BLAST227

Sequence similaritiesi

In the C-terminal section; belongs to the ABC-2 integral membrane protein family.Curated
Belongs to the ABC transporter superfamily.Curated
Contains 1 ABC transmembrane type-2 domain.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJ1. Bacteria.
COG0842. LUCA.
COG1131. LUCA.
HOGENOMiHOG000014886.
InParanoidiP37624.
KOiK13926.
OMAiAFTRTQI.
PhylomeDBiP37624.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000412. ABC_2_transport.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51012. ABC_TM2. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37624-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHLELVPVP PVAQLAGVSQ HYGKTVALNN ITLDIPARCM VGLIGPDGVG
60 70 80 90 100
KSSLLSLISG ARVIEQGNVM VLGGDMRDPK HRRDVCPRIA WMPQGLGKNL
110 120 130 140 150
YHTLSVYENV DFFARLFGHD KAEREVRINE LLTSTGLAPF RDRPAGKLSG
160 170 180 190 200
GMKQKLGLCC ALIHDPELLI LDEPTTGVDP LSRSQFWDLI DSIRQRQSNM
210 220 230 240 250
SVLVATAYME EAERFDWLVA MNAGEVLATG SAEELRQQTQ SATLEEAFIN
260 270 280 290 300
LLPQAQRQAH QAVVIPPYQP ENAEIAIEAR DLTMRFGSFV AVDHVNFRIP
310 320 330 340 350
RGEIFGFLGS NGCGKSTTMK MLTGLLPASE GEAWLFGQPV DPKDIDTRRR
360 370 380 390 400
VGYMSQAFSL YNELTVRQNL ELHARLFHIP EAEIPARVAE MSERFKLNDV
410 420 430 440 450
EDILPESLPL GIRQRLSLAV AVIHRPEMLI LDEPTSGVDP VARDMFWQLM
460 470 480 490 500
VDLSRQDKVT IFISTHFMNE AERCDRISLM HAGKVLASGT PQELVEKRGA
510 520 530 540 550
ASLEEAFIAY LQEAAGQSNE AEAPPVVHDT THAPRQGFSL RRLFSYSRRE
560 570 580 590 600
ALELRRDPVR STLALMGTVI LMLIMGYGIS MDVENLRFAV LDRDQTVSSQ
610 620 630 640 650
AWTLNLSGSR YFIEQPPLTS YDELDRRMRA GDITVAIEIP PNFGRDIARG
660 670 680 690 700
TPVELGVWID GAMPSRAETV KGYVQAMHQS WLQDVASRQS TPASQSGLMN
710 720 730 740 750
IETRYRYNPD VKSLPAIVPA VIPLLLMMIP SMLSALSVVR EKELGSIINL
760 770 780 790 800
YVTPTTRSEF LLGKQLPYIA LGMLNFFLLC GLSVFVFGVP HKGSFLTLTL
810 820 830 840 850
AALLYIIIAT GMGLLISTFM KSQIAAIFGT AIITLIPATQ FSGMIDPVAS
860 870 880 890 900
LEGPGRWIGE VYPTSHFLTI ARGTFSKALD LTDLWQLFIP LLIAIPLVMG
910
LSILLLKKQE G
Length:911
Mass (Da):100,785
Last modified:October 11, 2004 - v3
Checksum:i683E1EBB62E6B69E
GO

Sequence cautioni

The sequence AAB18462 differs from that shown. Reason: Frameshift at positions 10 and 241.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18461.1. Frameshift.
U00039 Genomic DNA. Translation: AAB18462.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48187.1.
AP009048 Genomic DNA. Translation: BAE77807.1.
PIRiA65146.
RefSeqiWP_000149156.1. NZ_LN832404.1.
YP_026225.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48187; AAT48187; b3486.
BAE77807; BAE77807; BAE77807.
GeneIDi947998.
KEGGiecj:JW5676.
eco:b3486.
PATRICi32122416. VBIEscCol129921_3585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18461.1. Frameshift.
U00039 Genomic DNA. Translation: AAB18462.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48187.1.
AP009048 Genomic DNA. Translation: BAE77807.1.
PIRiA65146.
RefSeqiWP_000149156.1. NZ_LN832404.1.
YP_026225.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP37624.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259649. 229 interactors.
DIPiDIP-12363N.
IntActiP37624. 1 interactor.
STRINGi511145.b3486.

Proteomic databases

PaxDbiP37624.
PRIDEiP37624.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48187; AAT48187; b3486.
BAE77807; BAE77807; BAE77807.
GeneIDi947998.
KEGGiecj:JW5676.
eco:b3486.
PATRICi32122416. VBIEscCol129921_3585.

Organism-specific databases

EchoBASEiEB2136.
EcoGeneiEG12223. rbbA.

Phylogenomic databases

eggNOGiENOG4105CJ1. Bacteria.
COG0842. LUCA.
COG1131. LUCA.
HOGENOMiHOG000014886.
InParanoidiP37624.
KOiK13926.
OMAiAFTRTQI.
PhylomeDBiP37624.

Enzyme and pathway databases

BioCyciEcoCyc:YHIH-MONOMER.
ECOL316407:JW5676-MONOMER.

Miscellaneous databases

PROiP37624.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000412. ABC_2_transport.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51012. ABC_TM2. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBBA_ECOLI
AccessioniPrimary (citable) accession number: P37624
Secondary accession number(s): P37625, Q2M7E9, Q6BF27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.