Reviewed, UniProtKB/Swiss-Prot P37623 (ACPT_ECOLI)

Names and origin

Protein names

Recommended name:
    4'-phosphopantetheinyl transferase acpT
    EC=2.7.8.-

Gene names

Name:	acpT
Synonyms:	yhhU
Ordered Locus Names:	b3475, JW3440

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in an alternative pathway for phosphopantetheinyl transfer and holo-ACP synthesis in E.coli. The native apo-protein substrate is unknown. Is able to functionally replace acpS in vivo but only when expressed at high levels. Ref.4 Ref.5
Catalytic activity	CoA + [protein-X] = adenosine 3',5'-bisphosphate + phosphopantetheinyl-[protein-X].
Sequence similarities	Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family.

Ontologies

Keywords
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	macromolecule biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	holo-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	4'-phosphopantetheinyl transferase acpT		PRO_0000206081

Sequences

	Sequence		Length	Mass (Da)
	P37623-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 29385FDA343B2AB7	FASTA	195	21,768
	10 20 30 40 50 60 MYRIVLGKVS TLSAAPLPPG LREQAPQGPR RERWLAGRAL LSHTLSPLPE IIYGEQGKPA 70 80 90 100 110 120 FAPEMPLWFN LSHSGDDIAL LLSDEGEVGC DIEVIRPRAN WRWLANAVFS LGEHAEMDAV 130 140 150 160 170 180 HPDQQLEMFW RIWTRKEAIV KQRGGSAWQI VSVDSTYHSS LSVSHCQLEN LSLAICTPTP 190 FTLTADSVQW IDSVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"A new enzyme superfamily -- the phosphopantetheinyl transferases." Lambalot R.H., Gehring A.M., Flugel R.S., Zuber P., LaCelle M., Marahiel M.A., Reid R., Khosla C., Walsh C.T. Chem. Biol. 3:923-936(1996) [PubMed: 8939709] [Abstract] Cited for: FUNCTION.
[5]	"Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli." Flugel R.S., Hwangbo Y., Lambalot R.H., Cronan J.E. Jr., Walsh C.T. J. Biol. Chem. 275:959-968(2000) [PubMed: 10625633] [Abstract] Cited for: FUNCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00039 Genomic DNA. Translation: AAB18450.1. U00096 Genomic DNA. Translation: AAC76500.1. AP009048 Genomic DNA. Translation: BAE77818.1.
PIR	S47694.
RefSeq	AP_004317.1. NP_417932.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:12355N.
Genome annotation databases
GeneID	947979.
GenomeReviews	Gene locus JW3440 in contig AP009048_GR. Gene locus b3475 in contig U00096_GR.
KEGG	ecj:JW3440. eco:b3475.
Organism-specific databases
EchoBASE	EB2135.
EcoGene	EG12221. acpT.
CMR	Search...
Phylogenomic databases
HOGENOM	P37623.
OMA	P37623. PLPEIVY.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12221-MON.
Family and domain databases
InterPro	IPR008278. 4-PPantetheinyl_Trfase. [Graphical view]
Pfam	PF01648. ACPS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACPT_ECOLI

Accession

Primary (citable) accession number: P37623
Secondary accession number(s): Q2M7D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)