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Protein

Uncharacterized MFS-type transporter YhhS

Gene

yhhS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Confers high-level resistance to glyphosate when overexpressed (PubMed:22089966). Overexpression has no effect on intracellular arabinose concentrations (PubMed:22952739).2 Publications

GO - Biological processi

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:YHHS-MONOMER.

Protein family/group databases

TCDBi2.A.1.46.7. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized MFS-type transporter YhhSUniRule annotationCurated
Gene namesi
Name:yhhS
Ordered Locus Names:b3473, JW5945
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12219. yhhS.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicCuratedAdd BLAST18
Transmembranei19 – 39HelicalUniRule annotationAdd BLAST21
Topological domaini40 – 46PeriplasmicCurated7
Transmembranei47 – 67HelicalUniRule annotationAdd BLAST21
Topological domaini68 – 84CytoplasmicCuratedAdd BLAST17
Transmembranei85 – 105HelicalUniRule annotationAdd BLAST21
Topological domaini106PeriplasmicCurated1
Transmembranei107 – 127HelicalUniRule annotationAdd BLAST21
Topological domaini128 – 155CytoplasmicCuratedAdd BLAST28
Transmembranei156 – 176HelicalUniRule annotationAdd BLAST21
Topological domaini177PeriplasmicCurated1
Transmembranei178 – 198HelicalUniRule annotationAdd BLAST21
Topological domaini199 – 223CytoplasmicCuratedAdd BLAST25
Transmembranei224 – 244HelicalUniRule annotationAdd BLAST21
Topological domaini245 – 251PeriplasmicCurated7
Transmembranei252 – 272HelicalUniRule annotationAdd BLAST21
Topological domaini273 – 282CytoplasmicCurated10
Transmembranei283 – 303HelicalUniRule annotationAdd BLAST21
Topological domaini304 – 308PeriplasmicCurated5
Transmembranei309 – 329HelicalUniRule annotationAdd BLAST21
Topological domaini330 – 343CytoplasmicCuratedAdd BLAST14
Transmembranei344 – 364HelicalUniRule annotationAdd BLAST21
Topological domaini365PeriplasmicCurated1
Transmembranei366 – 386HelicalUniRule annotationAdd BLAST21
Topological domaini387 – 405Cytoplasmic1 PublicationAdd BLAST19

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion increases intracellular arabinose concentrations as compared to the wild type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878071 – 405Uncharacterized MFS-type transporter YhhSAdd BLAST405

Proteomic databases

PaxDbiP37621.
PRIDEiP37621.

Expressioni

Inductioni

Induced by arabinose.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263334. 141 interactors.
STRINGi316385.ECDH10B_3647.

Structurei

3D structure databases

ProteinModelPortaliP37621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily. YhhS family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105F9N. Bacteria.
ENOG410XNX3. LUCA.
HOGENOMiHOG000122488.
InParanoidiP37621.
PhylomeDBiP37621.

Family and domain databases

CDDicd06174. MFS. 1 hit.
HAMAPiMF_01118. MFS_YhhS. 1 hit.
InterProiView protein in InterPro
IPR011701. MFS.
IPR020846. MFS_dom.
IPR023008. UPF0226_MFS.
PfamiView protein in Pfam
PF07690. MFS_1. 2 hits.
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiView protein in PROSITE
PS50850. MFS. 1 hit.

Sequencei

Sequence statusi: Complete.

P37621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEPVAEPAL NGLRLNLRIV SIVMFNFASY LTIGLPLAVL PGYVHDVMGF
60 70 80 90 100
SAFWAGLVIS LQYFATLLSR PHAGRYADSL GPKKIVVFGL CGCFLSGLGY
110 120 130 140 150
LTAGLTASLP VISLLLLCLG RVILGIGQSF AGTGSTLWGV GVVGSLHIGR
160 170 180 190 200
VISWNGIVTY GAMAMGAPLG VVFYHWGGLQ ALALIIMGVA LVAILLAIPR
210 220 230 240 250
PTVKASKGKP LPFRAVLGRV WLYGMALALA SAGFGVIATF ITLFYDAKGW
260 270 280 290 300
DGAAFALTLF SCAFVGTRLL FPNGINRIGG LNVAMICFSV EIIGLLLVGV
310 320 330 340 350
ATMPWMAKIG VLLAGAGFSL VFPALGVVAV KAVPQQNQGA ALATYTVFMD
360 370 380 390 400
LSLGVTGPLA GLVMSWAGVP VIYLAAAGLV AIALLLTWRL KKRPPEHVPE

AASSS
Length:405
Mass (Da):42,156
Last modified:December 4, 2007 - v2
Checksum:i4FC17F42FFAE51D1
GO

Sequence cautioni

The sequence AAB18448 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18448.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76498.2.
AP009048 Genomic DNA. Translation: BAE77820.1.
PIRiS47692.
RefSeqiNP_417930.2. NC_000913.3.
WP_001300943.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76498; AAC76498; b3473.
BAE77820; BAE77820; BAE77820.
GeneIDi947982.
KEGGiecj:JW5945.
eco:b3473.
PATRICifig|511145.12.peg.3572.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiYHHS_ECOLI
AccessioniPrimary (citable) accession number: P37621
Secondary accession number(s): Q2M7D6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 4, 2007
Last modified: July 5, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries