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Protein

Lead, cadmium, zinc and mercury-transporting ATPase

Gene

zntA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in export of lead, cadmium, zinc and mercury.

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).
ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58Zinc1
Metal bindingi59Zinc1
Metal bindingi62Zinc1
Active sitei4364-aspartylphosphate intermediateBy similarity1
Metal bindingi628MagnesiumPROSITE-ProRule annotation1
Metal bindingi632MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

  • ATPase activity Source: EcoliWiki
  • ATP binding Source: UniProtKB-KW
  • cadmium-exporting ATPase activity Source: EcoCyc
  • cobalt ion transmembrane transporter activity Source: EcoCyc
  • lead ion transmembrane transporter activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • nickel cation transmembrane transporter activity Source: EcoCyc
  • zinc-exporting ATPase activity Source: EcoCyc

GO - Biological processi

  • cobalt ion transport Source: EcoCyc
  • detoxification of zinc ion Source: EcoliWiki
  • lead ion transport Source: EcoCyc
  • response to cadmium ion Source: EcoliWiki
  • zinc II ion transport Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Cadmium, Magnesium, Mercury, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:YHHO-MONOMER.
ECOL316407:JW3434-MONOMER.
MetaCyc:YHHO-MONOMER.
SABIO-RKP37617.

Protein family/group databases

TCDBi3.A.3.6.2. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Lead, cadmium, zinc and mercury-transporting ATPase (EC:3.6.3.3, EC:3.6.3.5)
Gene namesi
Name:zntA
Synonyms:yhhO
Ordered Locus Names:b3469, JW3434
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12215. zntA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 124PeriplasmicSequence analysisAdd BLAST124
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21
Topological domaini146 – 191CytoplasmicSequence analysisAdd BLAST46
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Topological domaini213 – 356PeriplasmicSequence analysisAdd BLAST144
Transmembranei357 – 377HelicalSequence analysisAdd BLAST21
Topological domaini378 – 383CytoplasmicSequence analysis6
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21
Topological domaini405 – 461PeriplasmicSequence analysisAdd BLAST57
Transmembranei462 – 482HelicalSequence analysisAdd BLAST21
Topological domaini483 – 632CytoplasmicSequence analysisAdd BLAST150
Transmembranei633 – 653HelicalSequence analysisAdd BLAST21
Topological domaini654 – 693PeriplasmicSequence analysisAdd BLAST40
Transmembranei694 – 714HelicalSequence analysisAdd BLAST21
Topological domaini715 – 732CytoplasmicSequence analysisAdd BLAST18

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • integral component of plasma membrane Source: GO_Central
  • intracellular membrane-bounded organelle Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463321 – 732Lead, cadmium, zinc and mercury-transporting ATPaseAdd BLAST732

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP37617.
PaxDbiP37617.
PRIDEiP37617.

Interactioni

Protein-protein interaction databases

BioGridi4262921. 11 interactors.
DIPiDIP-12947N.
IntActiP37617. 8 interactors.
MINTiMINT-1256950.
STRINGi511145.b3469.

Structurei

Secondary structure

1732
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi48 – 56Combined sources9
Helixi62 – 71Combined sources10
Beta strandi73 – 82Combined sources10
Turni83 – 86Combined sources4
Beta strandi87 – 94Combined sources8
Helixi97 – 107Combined sources11
Beta strandi110 – 113Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MWYNMR-A46-118[»]
1MWZNMR-A46-118[»]
ProteinModelPortaliP37617.
SMRiP37617.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37617.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 113HMAPROSITE-ProRule annotationAdd BLAST65

Sequence similaritiesi

Contains 1 HMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C59. Bacteria.
COG2217. LUCA.
HOGENOMiHOG000250399.
InParanoidiP37617.
KOiK01534.
OMAiEPWETWI.
PhylomeDBiP37617.

Family and domain databases

CDDicd00371. HMA. 1 hit.
Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37617-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPDNHGKK APQFAAFKPL TTVQNANDCC CDGACSSTPT LSENVSGTRY
60 70 80 90 100
SWKVSGMDCA ACARKVENAV RQLAGVNQVQ VLFATEKLVV DADNDIRAQV
110 120 130 140 150
ESALQKAGYS LRDEQAAEEP QASRLKENLP LITLIVMMAI SWGLEQFNHP
160 170 180 190 200
FGQLAFIATT LVGLYPIARQ ALRLIKSGSY FAIETLMSVA AIGALFIGAT
210 220 230 240 250
AEAAMVLLLF LIGERLEGWA ASRARQGVSA LMALKPETAT RLRKGEREEV
260 270 280 290 300
AINSLRPGDV IEVAAGGRLP ADGKLLSPFA SFDESALTGE SIPVERATGD
310 320 330 340 350
KVPAGATSVD RLVTLEVLSE PGASAIDRIL KLIEEAEERR APIERFIDRF
360 370 380 390 400
SRIYTPAIMA VALLVTLVPP LLFAASWQEW IYKGLTLLLI GCPCALVIST
410 420 430 440 450
PAAITSGLAA AARRGALIKG GAALEQLGRV TQVAFDKTGT LTVGKPRVTA
460 470 480 490 500
IHPATGISES ELLTLAAAVE QGATHPLAQA IVREAQVAEL AIPTAESQRA
510 520 530 540 550
LVGSGIEAQV NGERVLICAA GKHPADAFTG LINELESAGQ TVVLVVRNDD
560 570 580 590 600
VLGVIALQDT LRADAATAIS ELNALGVKGV ILTGDNPRAA AAIAGELGLE
610 620 630 640 650
FKAGLLPEDK VKAVTELNQH APLAMVGDGI NDAPAMKAAA IGIAMGSGTD
660 670 680 690 700
VALETADAAL THNHLRGLVQ MIELARATHA NIRQNITIAL GLKGIFLVTT
710 720 730
LLGMTGLWLA VLADTGATVL VTANALRLLR RR
Length:732
Mass (Da):76,840
Last modified:October 1, 1994 - v1
Checksum:i25476EA830786465
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18444.1.
U00096 Genomic DNA. Translation: AAC76494.1.
AP009048 Genomic DNA. Translation: BAE77824.1.
PIRiS47688.
RefSeqiNP_417926.1. NC_000913.3.
WP_000106551.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76494; AAC76494; b3469.
BAE77824; BAE77824; BAE77824.
GeneIDi947972.
KEGGiecj:JW3434.
eco:b3469.
PATRICi32122382. VBIEscCol129921_3568.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18444.1.
U00096 Genomic DNA. Translation: AAC76494.1.
AP009048 Genomic DNA. Translation: BAE77824.1.
PIRiS47688.
RefSeqiNP_417926.1. NC_000913.3.
WP_000106551.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MWYNMR-A46-118[»]
1MWZNMR-A46-118[»]
ProteinModelPortaliP37617.
SMRiP37617.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262921. 11 interactors.
DIPiDIP-12947N.
IntActiP37617. 8 interactors.
MINTiMINT-1256950.
STRINGi511145.b3469.

Protein family/group databases

TCDBi3.A.3.6.2. the p-type atpase (p-atpase) superfamily.

Proteomic databases

EPDiP37617.
PaxDbiP37617.
PRIDEiP37617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76494; AAC76494; b3469.
BAE77824; BAE77824; BAE77824.
GeneIDi947972.
KEGGiecj:JW3434.
eco:b3469.
PATRICi32122382. VBIEscCol129921_3568.

Organism-specific databases

EchoBASEiEB2129.
EcoGeneiEG12215. zntA.

Phylogenomic databases

eggNOGiENOG4105C59. Bacteria.
COG2217. LUCA.
HOGENOMiHOG000250399.
InParanoidiP37617.
KOiK01534.
OMAiEPWETWI.
PhylomeDBiP37617.

Enzyme and pathway databases

BioCyciEcoCyc:YHHO-MONOMER.
ECOL316407:JW3434-MONOMER.
MetaCyc:YHHO-MONOMER.
SABIO-RKP37617.

Miscellaneous databases

EvolutionaryTraceiP37617.
PROiP37617.

Family and domain databases

CDDicd00371. HMA. 1 hit.
Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATZN_ECOLI
AccessioniPrimary (citable) accession number: P37617
Secondary accession number(s): Q2M7D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.