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P37586

- METH_SALTY

UniProt

P37586 - METH_SALTY

Protein

Methionine synthase

Gene

metH

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate By similarity.By similarity

    Catalytic activityi

    5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine.

    Cofactori

    Methylcobalamin (MeCBL).By similarity
    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi247 – 2471ZincPROSITE-ProRule annotation
    Metal bindingi310 – 3101ZincPROSITE-ProRule annotation
    Metal bindingi311 – 3111ZincPROSITE-ProRule annotation
    Metal bindingi759 – 7591Cobalt (cobalamin axial ligand)By similarity
    Binding sitei804 – 8041CobalaminBy similarity
    Binding sitei946 – 9461S-adenosyl-L-methionineBy similarity
    Binding sitei1134 – 11341S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei1138 – 11381Cobalamin; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. cobalamin binding Source: UniProtKB-KW
    2. methionine synthase activity Source: UniProtKB-EC
    3. S-adenosylmethionine-homocysteine S-methyltransferase activity Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. pteridine-containing compound metabolic process Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    Cobalamin, Cobalt, Metal-binding, S-adenosyl-L-methionine, Zinc

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-4218-MONOMER.
    UniPathwayiUPA00051; UER00081.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine synthase (EC:2.1.1.13)
    Alternative name(s):
    5-methyltetrahydrofolate--homocysteine methyltransferase
    Methionine synthase, vitamin-B12 dependent
    Short name:
    MS
    Gene namesi
    Name:metH
    Ordered Locus Names:STM4188
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 12271226Methionine synthasePRO_0000204536Add
    BLAST

    Proteomic databases

    PaxDbiP37586.
    PRIDEiP37586.

    Interactioni

    Protein-protein interaction databases

    STRINGi99287.STM4188.S.

    Structurei

    3D structure databases

    ProteinModelPortaliP37586.
    SMRiP37586. Positions 651-1227.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 325324Hcy-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini356 – 617262Pterin-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini650 – 74495B12-binding N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini746 – 881136B12-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini897 – 1227331AdoMet activationPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni834 – 8352Cobalamin-bindingBy similarity
    Regioni1189 – 11902S-adenosyl-L-methionine bindingBy similarity

    Domaini

    Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin By similarity.By similarity

    Sequence similaritiesi

    Contains 1 AdoMet activation domain.PROSITE-ProRule annotation
    Contains 1 B12-binding domain.PROSITE-ProRule annotation
    Contains 1 Hcy-binding domain.PROSITE-ProRule annotation
    Contains 1 pterin-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1410.
    KOiK00548.
    OMAiLTEHYAM.
    OrthoDBiEOG6091CH.
    PhylomeDBiP37586.

    Family and domain databases

    Gene3Di1.10.1240.10. 1 hit.
    3.10.196.10. 1 hit.
    3.20.20.20. 1 hit.
    3.20.20.330. 1 hit.
    3.40.50.280. 1 hit.
    InterProiIPR003759. Cbl-bd_cap.
    IPR006158. Cobalamin-bd.
    IPR011005. Dihydropteroate_synth-like.
    IPR011822. MetH.
    IPR000489. Pterin-binding.
    IPR003726. S_MeTrfase.
    IPR004223. VitB12-dep_Met_synth_activ_dom.
    [Graphical view]
    PfamiPF02310. B12-binding. 1 hit.
    PF02607. B12-binding_2. 1 hit.
    PF02965. Met_synt_B12. 1 hit.
    PF00809. Pterin_bind. 1 hit.
    PF02574. S-methyl_trans. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000381. MetH. 1 hit.
    SMARTiSM01018. B12-binding_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47644. SSF47644. 1 hit.
    SSF51717. SSF51717. 1 hit.
    SSF52242. SSF52242. 1 hit.
    SSF56507. SSF56507. 1 hit.
    SSF82282. SSF82282. 1 hit.
    TIGRFAMsiTIGR02082. metH. 1 hit.
    PROSITEiPS50974. ADOMET_ACTIVATION. 1 hit.
    PS51332. B12_BINDING. 1 hit.
    PS51337. B12_BINDING_NTER. 1 hit.
    PS50970. HCY. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P37586-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKVEQLRA QLNERILVLD GGMGTMIQSY RLHEEDFRGE RFADWPCDLK     50
    GNNDLLVLSK PEVIAAIHNA YFEAGADIIE TNTFNSTTIA MADYRMESLS 100
    AEINYAAAKL ARACADEWTA RTPEKPRFVA GVLGPTNRTA SISPDVNDPA 150
    FRNITFDQLV AAYRESTKAL VEGGADLILI ETVFDTLNAK AAVFAVKEEF 200
    EALGVDLPIM ISGTITDASG RTLSGQTTEA FYNSLRHAEA LTFGLNCALG 250
    PDELRQYVQE LSRIAECYVT AHPNAGLPNA FGEYDLDADT MAKQIREWAE 300
    AGFLNIVGGC CGTTPEHIAA MSRAVAGLLP RQLPDIPVAC RLSGLEPLNI 350
    GDDSLFVNVG ERTNVTGSAK FKRLIKEEKY SEALDVARQQ VESGAQIIDI 400
    NMDEGMLDAE AAMVRFLSLI AGEPDIARVP IMIDSSKWEV IEKGLKCIQG 450
    KGIVNSISMK EGVEAFIHHA KLLRRYGAAV VVMAFDEQGQ ADTRARKIEI 500
    CRRAYKILTE EVGFPPEDII FDPNIFAVAT GIEEHNNYAQ DFIGACEDIK 550
    RELPHALISG GVSNVSFSFR GNDPVREAIH AVFLYYAIRN GMDMGIVNAG 600
    QLAIYDDLPA ELRDAVEDVI LNRRDDGTER LLDLAEKYRG SKTDEAANAQ 650
    QAEWRSWDVK KRLEYSLVKG ITEFIEQDTE EARQQAARPI EVIEGPLMDG 700
    MNVVGDLFGE GKMFLPQVVK SARVMKQAVA YLEPFIEASK EKGSSNGKMV 750
    IATVKGDVHD IGKNIVGVVL QCNNYEIVDL GVMVPAEKIL RTAREVNADL 800
    IGLSGLITPS LDEMVNVAKE MERQGFTIPL LIGGATTSKA HTAVKIEQNY 850
    SGPTVYVQNA SRTVGVVAAL LSDTQRDDFV ARTRKEYETV RIQHARKKPR 900
    TPPVTLEAAR DNDLAFDWER YTPPVAHRLG VQEVEASIET LRNYIDWTPF 950
    FMTWSLAGKY PRILEDEVVG VEAQRLFKDA NDMLDKLSAE KLLNPRGVVG 1000
    LFPANRVGDD IEIYRDETRT HVLTVSHHLR QQTEKVGFAN YCLADFVAPK 1050
    LSGKADYIGA FAVTGGLEED ALADAFEAQH DDYNKIMVKA IADRLAEAFA 1100
    EYLHERVRKV YWGYAPNESL SNDELIRENY QGIRPAPGYP ACPEHTEKGT 1150
    IWQLLDVEKH TGMKLTESFA MWPGASVSGW YFSHPESKYF AVAQIQRDQV 1200
    TDYAFRKGMS VEDVERWLAP NLGYDAD 1227
    Length:1,227
    Mass (Da):136,004
    Last modified:January 23, 2007 - v3
    Checksum:i11C8E21745FF5354
    GO

    Sequence cautioni

    The sequence AAL23012.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti115 – 1151A → R(PubMed:3072256)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL23012.1. Different initiation.
    RefSeqiNP_463053.2. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL23012; AAL23012; STM4188.
    GeneIDi1255714.
    KEGGistm:STM4188.S.
    PATRICi32387269. VBISalEnt20916_4402.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL23012.1 . Different initiation.
    RefSeqi NP_463053.2. NC_003197.1.

    3D structure databases

    ProteinModelPortali P37586.
    SMRi P37586. Positions 651-1227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM4188.S.

    Proteomic databases

    PaxDbi P37586.
    PRIDEi P37586.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL23012 ; AAL23012 ; STM4188 .
    GeneIDi 1255714.
    KEGGi stm:STM4188.S.
    PATRICi 32387269. VBISalEnt20916_4402.

    Phylogenomic databases

    eggNOGi COG1410.
    KOi K00548.
    OMAi LTEHYAM.
    OrthoDBi EOG6091CH.
    PhylomeDBi P37586.

    Enzyme and pathway databases

    UniPathwayi UPA00051 ; UER00081 .
    BioCyci SENT99287:GCTI-4218-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1240.10. 1 hit.
    3.10.196.10. 1 hit.
    3.20.20.20. 1 hit.
    3.20.20.330. 1 hit.
    3.40.50.280. 1 hit.
    InterProi IPR003759. Cbl-bd_cap.
    IPR006158. Cobalamin-bd.
    IPR011005. Dihydropteroate_synth-like.
    IPR011822. MetH.
    IPR000489. Pterin-binding.
    IPR003726. S_MeTrfase.
    IPR004223. VitB12-dep_Met_synth_activ_dom.
    [Graphical view ]
    Pfami PF02310. B12-binding. 1 hit.
    PF02607. B12-binding_2. 1 hit.
    PF02965. Met_synt_B12. 1 hit.
    PF00809. Pterin_bind. 1 hit.
    PF02574. S-methyl_trans. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000381. MetH. 1 hit.
    SMARTi SM01018. B12-binding_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47644. SSF47644. 1 hit.
    SSF51717. SSF51717. 1 hit.
    SSF52242. SSF52242. 1 hit.
    SSF56507. SSF56507. 1 hit.
    SSF82282. SSF82282. 1 hit.
    TIGRFAMsi TIGR02082. metH. 1 hit.
    PROSITEi PS50974. ADOMET_ACTIVATION. 1 hit.
    PS51332. B12_BINDING. 1 hit.
    PS51337. B12_BINDING_NTER. 1 hit.
    PS50970. HCY. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    2. "The control region of the metH gene of Salmonella typhimurium LT2: an atypical met promoter."
      Urbanowski M.L., Stauffer G.V.
      Gene 73:193-200(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-371.
      Strain: LT2.

    Entry informationi

    Entry nameiMETH_SALTY
    AccessioniPrimary (citable) accession number: P37586
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 116 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    L-homocysteine is bound via the zinc atom.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3