Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable tRNA-dihydrouridine synthase 1

Gene

dus1

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.By similarity

Catalytic activityi

5,6-dihydrouracil in tRNA + NAD(P)+ = uracil in tRNA + NAD(P)H.By similarity

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71FMNBy similarity1
Active sitei102Proton donorBy similarity1
Binding sitei141FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 19FMNBy similarity3
Nucleotide bindingi202 – 204FMNBy similarity3
Nucleotide bindingi226 – 227FMNBy similarity2

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase, RNA-binding, tRNA-binding
Biological processtRNA processing
LigandFlavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciBSUB:BSU00810-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-dihydrouridine synthase 1 (EC:1.3.1.-)
Gene namesi
Name:dus1
Synonyms:yacF
Ordered Locus Names:BSU00810
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001621321 – 333Probable tRNA-dihydrouridine synthase 1Add BLAST333

Proteomic databases

PaxDbiP37567
PRIDEiP37567

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100000410

Structurei

3D structure databases

ProteinModelPortaliP37567
SMRiP37567
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family.Curated

Phylogenomic databases

eggNOGiENOG4105CEH Bacteria
COG0042 LUCA
HOGENOMiHOG000217853
InParanoidiP37567
OMAiRHYTNYF
PhylomeDBiP37567

Family and domain databases

CDDicd02801 DUS_like_FMN, 1 hit
Gene3Di1.10.1200.80, 1 hit
3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035587 DUS-like_FMN-bd
IPR024036 tRNA-dHydroUridine_Synthase_C
IPR004652 tRNA_dU_NifR3
IPR001269 tRNA_hU_synthase
IPR018517 tRNA_hU_synthase_CS
PANTHERiPTHR11082 PTHR11082, 1 hit
PTHR11082:SF25 PTHR11082:SF25, 1 hit
PfamiView protein in Pfam
PF01207 Dus, 1 hit
PIRSFiPIRSF006621 Dus, 1 hit
TIGRFAMsiTIGR00737 nifR3_yhdG, 1 hit
PROSITEiView protein in PROSITE
PS01136 UPF0034, 1 hit

Sequencei

Sequence statusi: Complete.

P37567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKIGDIQLK NRVVLAPMAG VCNSAFRLTV KEFGAGLVCA EMVSDKAILY
60 70 80 90 100
NNARTMGMLY IDEREKPLSL QIFGGKKETL VEAAKFVDQN TTADIIDINM
110 120 130 140 150
GCPVPKITKC DAGAKWLLDP DKIYEMVSAV VDAVNKPVTV KMRMGWDEDH
160 170 180 190 200
IFAVKNAQAV ERAGGKAVAL HGRTRVQMYE GTANWDIIKE VKQSVSIPVI
210 220 230 240 250
GNGDVKTPQD AKRMLDETGV DGVMIGRAAL GNPWMIYRTV QYLETGKLKE
260 270 280 290 300
EPQVREKMAV CKLHLDRLID LKGENVAVRE MRKHAAWYLK GVKGNANVRN
310 320 330
EINHCETREE FVQLLDAFTV EVEAKELQNA KVG
Length:333
Mass (Da):37,066
Last modified:October 1, 1994 - v1
Checksum:i73CC58A422008EFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA Translation: BAA05315.1
AL009126 Genomic DNA Translation: CAB11857.1
PIRiS66110
RefSeqiNP_387962.1, NC_000964.3
WP_003243741.1, NZ_JNCM01000028.1

Genome annotation databases

EnsemblBacteriaiCAB11857; CAB11857; BSU00810
GeneIDi936924
KEGGibsu:BSU00810
PATRICifig|224308.179.peg.81

Similar proteinsi

Entry informationi

Entry nameiDUS1_BACSU
AccessioniPrimary (citable) accession number: P37567
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 25, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health