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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.1 Publication

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 30ATPSequence analysis6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU00670-MONOMER.
BRENDAi6.3.4.19. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthase (EC:6.3.4.19)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase
Gene namesi
Name:tilS
Synonyms:yacA
Ordered Locus Names:BSU00670
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001816501 – 472tRNA(Ile)-lysidine synthaseAdd BLAST472

Proteomic databases

PaxDbiP37563.
PRIDEiP37563.

Interactioni

Protein-protein interaction databases

IntActiP37563. 2 interactors.
STRINGi224308.Bsubs1_010100000340.

Structurei

3D structure databases

ProteinModelPortaliP37563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236506.
InParanoidiP37563.
KOiK04075.
OMAiNDISYPL.
PhylomeDBiP37563.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR012796. Lysidine-tRNA-synth_C.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
IPR015262. tRNA_Ile_lys_synt_subst-bd.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

P37563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSVKDFLNK HNLTLKGATI IVGVSGGPDS MALLHALHTL CGRSANVIAA
60 70 80 90 100
HVDHRFRGAE SEEDMRFVQA YCKAEQLVCE TAQINVTAYA QEKGLNKQAA
110 120 130 140 150
ARDCRYQFFE EIMSKHQADY LALAHHGDDQ VETMLMKLAK GTLGTGLAGM
160 170 180 190 200
QPVRRFGTGR IIRPFLTITK EEILHYCHEN GLSYRTDESN AKDDYTRNRF
210 220 230 240 250
RKTVLPFLKQ ESPDVHKRFQ KVSEALTEDE QFLQSLTKDE MNKVITSQSN
260 270 280 290 300
TSVEINSSQL LALPMPLQRR GVQLILNYLY ENVPSSFSAH HIQQFLDWAE
310 320 330 340 350
NGGPSGVLDF PKGLKVVKSY QTCLFTFEQW QCKNVPFEYQ ISGAADETAV
360 370 380 390 400
LPNGYLIEAR HYADSPEEHG NAVFITSEKK VRFPLTIRTR KAGDRIKLKG
410 420 430 440 450
MNGSKKVKDI FIDKKLPLQE RDNWPIVTDA SGEIIWIPGL KKSIFEDLVI
460 470
PNSDRIVLQY RQHEKCRGQA KS
Length:472
Mass (Da):53,464
Last modified:May 30, 2000 - v2
Checksum:i2723EEA0B6F4252D
GO

Sequence cautioni

The sequence BAA05302 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05302.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB11843.1.
PIRiS66097.
RefSeqiNP_387948.1. NC_000964.3.
WP_003243704.1. NZ_JNCM01000028.1.

Genome annotation databases

EnsemblBacteriaiCAB11843; CAB11843; BSU00670.
GeneIDi936922.
KEGGibsu:BSU00670.
PATRICi18971609. VBIBacSub10457_0068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05302.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB11843.1.
PIRiS66097.
RefSeqiNP_387948.1. NC_000964.3.
WP_003243704.1. NZ_JNCM01000028.1.

3D structure databases

ProteinModelPortaliP37563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP37563. 2 interactors.
STRINGi224308.Bsubs1_010100000340.

Proteomic databases

PaxDbiP37563.
PRIDEiP37563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11843; CAB11843; BSU00670.
GeneIDi936922.
KEGGibsu:BSU00670.
PATRICi18971609. VBIBacSub10457_0068.

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236506.
InParanoidiP37563.
KOiK04075.
OMAiNDISYPL.
PhylomeDBiP37563.

Enzyme and pathway databases

BioCyciBSUB:BSU00670-MONOMER.
BRENDAi6.3.4.19. 658.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR012796. Lysidine-tRNA-synth_C.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
IPR015262. tRNA_Ile_lys_synt_subst-bd.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTILS_BACSU
AccessioniPrimary (citable) accession number: P37563
Secondary accession number(s): O31416
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 30, 2000
Last modified: October 5, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.