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P37563 (TILS_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA(Ile)-lysidine synthase
EC=6.3.4.19
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase
Gene names
Name:tilS
Synonyms:yacA
Ordered Locus Names:BSU00670
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. Ref.3

Catalytic activity

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O. Ref.3

Subcellular location

Cytoplasm Probable HAMAP MF_01161.

Domain

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. HAMAP MF_01161

Sequence similarities

Belongs to the tRNA(Ile)-lysidine synthase family.

Sequence caution

The sequence BAA05302.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ligase activity, forming carbon-nitrogen bonds

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472tRNA(Ile)-lysidine synthase HAMAP MF_01161
PRO_0000181650

Regions

Nucleotide binding25 – 306ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P37563 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 2723EEA0B6F4252D

FASTA47253,464
        10         20         30         40         50         60 
MKSVKDFLNK HNLTLKGATI IVGVSGGPDS MALLHALHTL CGRSANVIAA HVDHRFRGAE 

        70         80         90        100        110        120 
SEEDMRFVQA YCKAEQLVCE TAQINVTAYA QEKGLNKQAA ARDCRYQFFE EIMSKHQADY 

       130        140        150        160        170        180 
LALAHHGDDQ VETMLMKLAK GTLGTGLAGM QPVRRFGTGR IIRPFLTITK EEILHYCHEN 

       190        200        210        220        230        240 
GLSYRTDESN AKDDYTRNRF RKTVLPFLKQ ESPDVHKRFQ KVSEALTEDE QFLQSLTKDE 

       250        260        270        280        290        300 
MNKVITSQSN TSVEINSSQL LALPMPLQRR GVQLILNYLY ENVPSSFSAH HIQQFLDWAE 

       310        320        330        340        350        360 
NGGPSGVLDF PKGLKVVKSY QTCLFTFEQW QCKNVPFEYQ ISGAADETAV LPNGYLIEAR 

       370        380        390        400        410        420 
HYADSPEEHG NAVFITSEKK VRFPLTIRTR KAGDRIKLKG MNGSKKVKDI FIDKKLPLQE 

       430        440        450        460        470 
RDNWPIVTDA SGEIIWIPGL KKSIFEDLVI PNSDRIVLQY RQHEKCRGQA KS

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
Ogasawara N., Nakai S., Yoshikawa H.
DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA."
Soma A., Ikeuchi Y., Kanemasa S., Kobayashi K., Ogasawara N., Ote T., Kato J., Watanabe K., Sekine Y., Suzuki T.
Mol. Cell 12:689-698(2003) [PubMed: 14527414] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D26185 Genomic DNA. Translation: BAA05302.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB11843.1.
PIRS66097.
RefSeqNP_387948.1. NC_000964.3.

3D structure databases

ProteinModelPortalP37563.
SMRP37563. Positions 1-463.
ModBaseSearch...

Protein-protein interaction databases

IntActP37563. 4 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000002327; EBBACP00000002327; EBBACG00000002322.
GeneID936922.
GenomeReviewsGene locus BSU00670 in contig AL009126_GR.
KEGGbsu:BSU00670.
NMPDRfig|224308.1.peg.67.
PATRIC18971609. VBIBacSub10457_0068.

Organism-specific databases

GenoListBSU00670. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000002021.
HOGENOMHBG668702.
OMANDISYPL.
PhylomeDBP37563.
ProtClustDBCLSK886565.

Enzyme and pathway databases

BioCycBSUB:BSU00670-MONOMER.

Family and domain databases

HAMAPMF_01161. tRNA(Ile)-lys_synt.
[Tree]
InterProIPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR015262. Lysidine-tRNA-synth_subst-bd.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK04075.
PANTHERPTHR11807:SF2. ATPase_MesJ_YaeO. 1 hit.
PfamPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMSSF56037. B3_4. 1 hit.
TIGRFAMsTIGR02433. Lysidine_TilS_C. 1 hit.
TIGR02432. Lysidine_TilS_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTILS_BACSU
AccessionPrimary (citable) accession number: P37563
Secondary accession number(s): O31416
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 30, 2000
Last modified: January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents