Reviewed, UniProtKB/Swiss-Prot P37563 (TILS_BACSU)

Names and origin

Protein names

Recommended name:
    tRNA(Ile)-lysidine synthase
    EC=6.3.4.-
Alternative name(s):
    tRNA(Ile)-lysidine synthetase
    tRNA(Ile)-2-lysyl-cytidine synthase

Gene names

Name:	tilS
Synonyms:	yacA
Ordered Locus Names:	BSU00670

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. Ref.3
Subcellular location	Cytoplasm Probable.
Domain	The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. HAMAP MF_01161
Sequence similarities	Belongs to the tRNA(Ile)-lysidine synthase family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity, forming carbon-nitrogen bonds Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	tRNA(Ile)-lysidine synthase HAMAP MF_01161		PRO_0000181650
Regions
	Nucleotide binding	25 – 30	6	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P37563-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 2723EEA0B6F4252D	FASTA	472	53,464
	10 20 30 40 50 60 MKSVKDFLNK HNLTLKGATI IVGVSGGPDS MALLHALHTL CGRSANVIAA HVDHRFRGAE 70 80 90 100 110 120 SEEDMRFVQA YCKAEQLVCE TAQINVTAYA QEKGLNKQAA ARDCRYQFFE EIMSKHQADY 130 140 150 160 170 180 LALAHHGDDQ VETMLMKLAK GTLGTGLAGM QPVRRFGTGR IIRPFLTITK EEILHYCHEN 190 200 210 220 230 240 GLSYRTDESN AKDDYTRNRF RKTVLPFLKQ ESPDVHKRFQ KVSEALTEDE QFLQSLTKDE 250 260 270 280 290 300 MNKVITSQSN TSVEINSSQL LALPMPLQRR GVQLILNYLY ENVPSSFSAH HIQQFLDWAE 310 320 330 340 350 360 NGGPSGVLDF PKGLKVVKSY QTCLFTFEQW QCKNVPFEYQ ISGAADETAV LPNGYLIEAR 370 380 390 400 410 420 HYADSPEEHG NAVFITSEKK VRFPLTIRTR KAGDRIKLKG MNGSKKVKDI FIDKKLPLQE 430 440 450 460 470 RDNWPIVTDA SGEIIWIPGL KKSIFEDLVI PNSDRIVLQY RQHEKCRGQA KS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA." Soma A., Ikeuchi Y., Kanemasa S., Kobayashi K., Ogasawara N., Ote T., Kato J., Watanabe K., Sekine Y., Suzuki T. Mol. Cell 12:689-698(2003) [PubMed: 14527414] [Abstract] Cited for: FUNCTION, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D26185 Genomic DNA. Translation: BAA05302.1. Different initiation. AL009126 Genomic DNA. Translation: CAB11843.1.
PIR	S66097.
RefSeq	NP_387948.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	936922.
GenomeReviews	Gene locus BSU00670 in contig AL009126_GR.
KEGG	bsu:BSU00670.
NMPDR	fig|224308.1.peg.67.
Organism-specific databases
SubtiList	BG10130. tilS. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG668702.
OMA	TILMRLV.
Family and domain databases
HAMAP	MF_01161. [Tree]
InterPro	IPR012094. Lysidine-tRNA-synth. IPR012796. Lysidine-tRNA-synth_C. IPR012795. Lysidine-tRNA-synth_N. IPR015262. Lysidine-tRNA-synth_subst-bd. IPR011063. PP_loop. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11807:SF2. ATPase_MesJ_YaeO. 1 hit.
Pfam	PF01171. ATP_bind_3. 1 hit. PF09179. DUF1946. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TILS_BACSU

Accession

Primary (citable) accession number: P37563
Secondary accession number(s): O31416

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	May 30, 2000
Last modified:	December 15, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)