P37551 (PURR_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pur operon repressor

Gene names

Name:	purR
Synonyms:	yabI
Ordered Locus Names:	BSU00470

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Controls the transcription of the pur operon for purine biosynthetic genes, binds to the control region of the operon. DNA binding is inhibited by 5-phosphoribosyl 1-pyrophosphate.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of purine nucleobase metabolic process Inferred from electronic annotation. Source: InterPro negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro nucleoside metabolic process Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 285

285

Pur operon repressor

PRO_0000139699

Secondary structure

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285

Helix Strand Turn

Details...

Helix

5 – 17

13

Helix

26 – 32

7

Helix

37 – 53

17

Beta strand

56 – 62

7

Beta strand

68 – 73

6

Helix

77 – 91

15

Helix

94 – 96

3

Turn

99 – 101

3

Turn

106 – 110

5

Helix

112 – 125

14

Turn

126 – 128

3

Beta strand

132 – 137

6

Turn

138 – 141

4

Helix

142 – 152

11

Beta strand

156 – 160

5

Beta strand

170 – 176

7

Beta strand

182 – 189

8

Helix

190 – 192

3

Beta strand

198 – 209

12

Helix

210 – 218

9

Helix

220 – 222

3

Beta strand

225 – 237

13

Beta strand

247 – 253

7

Beta strand

257 – 260

4

Beta strand

263 – 266

4

Helix

269 – 272

4

Sequences

	Sequence		Length	Mass (Da)
	P37551 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 76B1DB289A3F34FB	FASTA	285	31,229
	10 20 30 40 50 60 MKFRRSGRLV DLTNYLLTHP HELIPLTFFS ERYESAKSSI SEDLTIIKQT FEQQGIGTLL 70 80 90 100 110 120 TVPGAAGGVK YIPKMKQAEA EEFVQTLGQS LANPERILPG GYVYLTDILG KPSVLSKVGK 130 140 150 160 170 180 LFASVFAERE IDVVMTVATK GIPLAYAAAS YLNVPVVIVR KDNKVTEGST VSINYVSGSS 190 200 210 220 230 240 NRIQTMSLAK RSMKTGSNVL IIDDFMKAGG TINGMINLLD EFNANVAGIG VLVEAEGVDE 250 260 270 280 RLVDEYMSLL TLSTINMKEK SIEIQNGNFL RFFKDNLLKN GETES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Identification of the Bacillus subtilis pur operon repressor." Weng M., Nagy P.L., Zalkin H. Proc. Natl. Acad. Sci. U.S.A. 92:7455-7459(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25, CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D26185 Genomic DNA. Translation: BAA05282.1.
AL009126 Genomic DNA. Translation: CAB11823.1.

PIR

S66076.

RefSeq

NP_387928.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1O57	X-ray	2.20	A/B/C/D	1-285	[»]
1P4A	X-ray	2.22	A/B/C/D	1-285	[»]

ProteinModelPortal

P37551.

SMR

P37551. Positions 2-273.

ModBase

Search...

Protein-protein interaction databases

STRING

224308.BSU00470.

Proteomic databases

PaxDb

P37551.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB11823; CAB11823; BSU00470.

GeneID

937000.

KEGG

bsu:BSU00470.

PATRIC

18971565. VBIBacSub10457_0048.

Organism-specific databases

GenoList

BSU00470. [Micado]

Phylogenomic databases

eggNOG

COG0503.

HOGENOM

HOG000205992.

KO

K09685.

OMA

MERPHTL.

ProtClustDB

PRK09213.

Enzyme and pathway databases

BioCyc

BSUB:BSU00470-MONOMER.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR000836. PRibTrfase_dom.
IPR015265. PuR_N.
IPR010078. PurR_Bsub.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00156. Pribosyltran. 1 hit.
PF09182. PuR_N. 1 hit.
[Graphical view]

ProDom

PD029816. PuR_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01743. purR_Bsub. 1 hit.

PROSITE

PS00103. PUR_PYR_PR_TRANSFER. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P37551.

Entry information

Entry name

PURR_BACSU

Accession

Primary (citable) accession number: P37551

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program