P37551 (PURR_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pur operon repressor

Gene names

Name:	purR
Synonyms:	yabI
Ordered Locus Names:	BSU00470

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Controls the transcription of the pur operon for purine biosynthetic genes, binds to the control region of the operon. DNA binding is inhibited by 5-phosphoribosyl 1-pyrophosphate.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of purine base metabolic process Inferred from electronic annotation. Source: InterPro negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro nucleoside metabolic process Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 285

285

Pur operon repressor

PRO_0000139699

Secondary structure

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285

Helix Strand Turn

Details...

Helix

5 – 17

13

Helix

26 – 32

7

Helix

37 – 53

17

Beta strand

56 – 62

7

Beta strand

68 – 73

6

Helix

77 – 91

15

Helix

94 – 96

3

Turn

99 – 101

3

Turn

106 – 110

5

Helix

112 – 125

14

Turn

126 – 128

3

Beta strand

132 – 137

6

Turn

138 – 141

4

Helix

142 – 152

11

Beta strand

156 – 160

5

Beta strand

170 – 176

7

Beta strand

182 – 189

8

Helix

190 – 192

3

Beta strand

198 – 209

12

Helix

210 – 218

9

Helix

220 – 222

3

Beta strand

225 – 237

13

Beta strand

247 – 253

7

Beta strand

257 – 260

4

Beta strand

263 – 266

4

Helix

269 – 272

4

Sequences

	Sequence		Length	Mass (Da)
	P37551 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 76B1DB289A3F34FB	FASTA	285	31,229
	10 20 30 40 50 60 MKFRRSGRLV DLTNYLLTHP HELIPLTFFS ERYESAKSSI SEDLTIIKQT FEQQGIGTLL 70 80 90 100 110 120 TVPGAAGGVK YIPKMKQAEA EEFVQTLGQS LANPERILPG GYVYLTDILG KPSVLSKVGK 130 140 150 160 170 180 LFASVFAERE IDVVMTVATK GIPLAYAAAS YLNVPVVIVR KDNKVTEGST VSINYVSGSS 190 200 210 220 230 240 NRIQTMSLAK RSMKTGSNVL IIDDFMKAGG TINGMINLLD EFNANVAGIG VLVEAEGVDE 250 260 270 280 RLVDEYMSLL TLSTINMKEK SIEIQNGNFL RFFKDNLLKN GETES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Identification of the Bacillus subtilis pur operon repressor." Weng M., Nagy P.L., Zalkin H. Proc. Natl. Acad. Sci. U.S.A. 92:7455-7459(1995) [PubMed: 7638212] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25, CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D26185 Genomic DNA. Translation: BAA05282.1.
AL009126 Genomic DNA. Translation: CAB11823.1.

PIR

S66076.

RefSeq

NP_387928.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1O57	X-ray	2.20	A/B/C/D	1-285	[»]
1P4A	X-ray	2.22	A/B/C/D	1-285	[»]

ProteinModelPortal

P37551.

SMR

P37551. Positions 2-273.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000001965; EBBACP00000001965; EBBACG00000001962.

GeneID

937000.

GenomeReviews

Gene locus BSU00470 in contig AL009126_GR.

KEGG

bsu:BSU00470.

NMPDR

fig|224308.1.peg.47.

PATRIC

18971565. VBIBacSub10457_0048.

Organism-specific databases

GenoList

BSU00470. [Micado]

Phylogenomic databases

GeneTree

EBGT00050000001498.

HOGENOM

HBG304155.

OMA

FKGQKID.

PhylomeDB

P37551.

ProtClustDB

PRK09213.

Enzyme and pathway databases

BioCyc

BSUB:BSU00470-MONOMER.

Family and domain databases

InterPro

IPR000836. PRibTrfase.
IPR015265. PuR_N.
IPR010078. PurR_Bsub.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

KO

K09685.

Pfam

PF00156. Pribosyltran. 1 hit.
PF09182. PuR_N. 1 hit.
[Graphical view]

ProDom

PD029816. PuR_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01743. PurR_Bsub. 1 hit.

PROSITE

PS00103. PUR_PYR_PR_TRANSFER. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PURR_BACSU

Accession

Primary (citable) accession number: P37551

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program