P37548 (YABG_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sporulation-specific protease yabG
EC=3.4.-.-

Gene names

Name:	yabG
Ordered Locus Names:	BSU00430

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cleaves the spore coat proteins spoIVA and safA. May cooperate with tgl to mediate the temperature-dependent cross-linking of coat proteins like gerQ. Ref.3 Ref.5
Subcellular location	Forespore outer membrane. Note: Synthesized in the mother cell compartment and assembled on the surface of the forespore. Ref.4
Developmental stage	Expressed in the mother cell compartment from T4 of sporulation.
Induction	Induced by sigK.
Disruption phenotype	According to Ref.4, cells have an altered coat protein composition; CotT, YeeK, YxeE, CotF, SafA and SpoIVA proteins are present at increased levels and/or exist as precursors. According to Ref.5, cells show no effects on vegetative growth or spore resistance to heat, chloroform or lysozyme. The germination of YabG mutant spores in L-alanine and in a mixture of L-asparagine, D-glucose, D-fructose and potassium chloride was the same as that of the wild-type spores. Heat treatment for 20 minutes at 60 degrees Celsius, which maximally activates the Tgl enzymatic activity, causes cross-linking of GerQ in isolated YabG-deleted spores but not in Tgl/YabG double-mutant spores. Additionally, the germination frequency of the Tgl/YabG double-mutant spores in the presence of L-alanine with or without heat activation at 60 degrees Celsius is lower than that of wild-type spores. Ref.4 Ref.5
Sequence similarities	Belongs to the peptidase U57 family.

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Membrane
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Sporulation-specific protease yabG		PRO_0000049441

Sequences

	Sequence		Length	Mass (Da)
	P37548 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: B60A5B9F9D3209BB	FASTA	290	33,318
	10 20 30 40 50 60 MQFQIGDMVA RKSYQMDVLF RIIGIEQTSK GNSIAILHGD EVRLIADSDF SDLVAVKKDE 70 80 90 100 110 120 QMMRKKKDES RMNESLELLR QDYKLLREKQ EYYATSQYQH QEHYFHMPGK VLHLDGDEAY 130 140 150 160 170 180 LKKCLNVYKK IGVPVYGIHC HEKKMSASIE VLLDKYRPDI LVITGHDAYS KQKGGIDDLN 190 200 210 220 230 240 AYRHSKHFVE TVQTARKKIP HLDQLVIFAG ACQSHFESLI RAGANFASSP SRVNIHALDP 250 260 270 280 290 VYIVAKISFT PFMERINVWE VLRNTLTREK GLGGIETRGV LRIGMPYKSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"The yabG gene of Bacillus subtilis encodes a sporulation specific protease which is involved in the processing of several spore coat proteins." Takamatsu H., Imamura A., Kodama T., Asai K., Ogasawara N., Watabe K. FEMS Microbiol. Lett. 192:33-38(2000) [PubMed: 11040425] [Abstract] Cited for: FUNCTION. Strain: 168.
[4]	"The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition." Takamatsu H., Kodama T., Imamura A., Asai K., Kobayashi K., Nakayama T., Ogasawara N., Watabe K. J. Bacteriol. 182:1883-1888(2000) [PubMed: 10714992] [Abstract] Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TRANSCRIPTION. Strain: 168.
[5]	"Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores." Kuwana R., Okuda N., Takamatsu H., Watabe K. J. Biochem. 139:887-901(2006) [PubMed: 16751597] [Abstract] Cited for: POSSIBLE ROLE IN CROSS-LINKING OF GERQ, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26185 Genomic DNA. Translation: BAA05278.1. AL009126 Genomic DNA. Translation: CAB11819.1.
PIR	S66072.
RefSeq	NP_387924.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P37548.
ModBase	Search...
Protein family/group databases
MEROPS	U57.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000001871; EBBACP00000001871; EBBACG00000001868.
GeneID	937005.
GenomeReviews	Gene locus BSU00430 in contig AL009126_GR.
KEGG	bsu:BSU00430.
NMPDR	fig|224308.1.peg.43.
PATRIC	18971557. VBIBacSub10457_0044.
Organism-specific databases
GenoList	BSU00430.
Phylogenomic databases
GeneTree	EBGT00050000002877.
HOGENOM	HBG313995.
OMA	ALDPVYI.
ProtClustDB	CLSK872603.
Enzyme and pathway databases
BioCyc	BSUB:BSU00430-MONOMER.
Family and domain databases
InterPro	IPR008764. Peptidase_U57. [Graphical view]
KO	K06436.
Pfam	PF05582. Peptidase_U57. 1 hit. [Graphical view]
ProDom	PD127708. Peptidase_U57. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02855. Spore_yabG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

YABG_BACSU

Accession

Primary (citable) accession number: P37548

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program