Reviewed, UniProtKB/Swiss-Prot P37540 (HOLB_BACSU)

Names and origin

Protein names

Recommended name:
    DNA polymerase III subunit delta'
    EC=2.7.7.7

Gene names

Name:	holB
Synonyms:	yaaS
Ordered Locus Names:	BSU00310

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Ontologies

Keywords
Biological process	DNA replication
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	DNA polymerase III subunit delta'		PRO_0000105509

Sequences

	Sequence		Length	Mass (Da)
	P37540-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 23F97A1E11FA22E5	FASTA	329	37,621
	10 20 30 40 50 60 MAISWKEMNE LQPRVMKLLY NSIEKDRLSH AYLFEGKKGT GKLDAALLLA KSFFCLEGGA 70 80 90 100 110 120 EPCESCRNCK RIESGNHPDL HLVQPDGLSI KKAQIQALQE EFSKTGLESH KKLYIISHAD 130 140 150 160 170 180 QMTANAANSL LKFLEEPNKD TMAVLITEQP QRLLDTIISR CQTLPFQPLQ PKAIEDRLIE 190 200 210 220 230 240 QDVSPHMARL LANMTNNVAE AVELSRNDEF AESRAKVIKL YEVLHQRKGH AFFFIQDQWM 250 260 270 280 290 300 PFFKEKTHQE MGLDMLLLIY RDVLSIQIGN EDKLIYQDLF QSIKQHALQS TQQSVTNQIL 310 320 AVLEAKKRLH SNVNVQGLME HLVLMLQEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D26185 Genomic DNA. Translation: BAA05267.1. AL009126 Genomic DNA. Translation: CAB11807.1.
PIR	S66061.
RefSeq	NP_387912.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	935949.
GenomeReviews	Gene locus BSU00310 in contig AL009126_GR.
KEGG	bsu:BSU00310.
NMPDR	fig|224308.1.peg.31.
Organism-specific databases
SubtiList	BG10095. holB. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P37540.
OMA	LMEQLVL
Enzyme and pathway databases
BRENDA	2.7.7.7. 150.
Family and domain databases
InterPro	IPR004622. DNA_pol_HolB. [Graphical view]
TIGRFAMs	TIGR00678. holB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HOLB_BACSU

Accession

Primary (citable) accession number: P37540

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)