Reviewed, UniProtKB/Swiss-Prot P37537 (KTHY_BACSU)

Names and origin

Protein names

Recommended name:
    Thymidylate kinase
    EC=2.7.4.9
Alternative name(s):
    dTMP kinase

Gene names

Name:	tmk
Synonyms:	yaaP
Ordered Locus Names:	BSU00280

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis. Ref.4
Catalytic activity	ATP + dTMP = ADP + dTDP. HAMAP MF_00165
Sequence similarities	Belongs to the thymidylate kinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTDP biosynthetic process Inferred from electronic annotation. Source: InterPro dTTP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidylate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Thymidylate kinase HAMAP MF_00165		PRO_0000155238
Regions
	Nucleotide binding	10 – 17	8	ATP Potential
Experimental info
	Sequence conflict	101	1	S → P in BAA05264. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37537-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 9763C20FA7F1DE85	FASTA	212	23,866
	10 20 30 40 50 60 MSGLFITFEG PEGAGKTTVL QEIKNILTAE GLQVMATREP GGIDIAEQIR EVILNENNIL 70 80 90 100 110 120 MDPKTEALLY AAARRQHLVE KVKPALEQGF IVLCDRFIDS SLAYQGYARG LGIDEVLSIN 130 140 150 160 170 180 EFAIGDMMPH VTVYFSIDPE EGLKRIYANG SREKNRLDLE KLDFHTKVQE GYQELMKRFP 190 200 210 ERFHSVDAGQ SKDLVVQDVL KVIDEALKKI QL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 101.
[4]	Gilles A.M., Barzu O. Unpublished observations (JUN-1996) Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D26185 Genomic DNA. Translation: BAA05264.1. AL009126 Genomic DNA. Translation: CAB11804.2.
PIR	S66058.
RefSeq	NP_387909.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	937018.
GenomeReviews	Gene locus BSU00280 in contig AL009126_GR.
KEGG	bsu:BSU00280.
NMPDR	fig|224308.1.peg.28.
Organism-specific databases
SubtiList	BG10092. tmk. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P37537.
Enzyme and pathway databases
BRENDA	2.7.4.9. 150.
Family and domain databases
HAMAP	MF_00165. [Tree]
InterPro	IPR018094. Thymidylate_kin. IPR000062. Thymidylate_kin-like. IPR018095. Thymidylate_kin_CS. [Graphical view]
PANTHER	PTHR10344. Thymidylate_kin. 1 hit.
Pfam	PF02223. Thymidylate_kin. 1 hit. [Graphical view]
TIGRFAMs	TIGR00041. DTMP_kinase. 1 hit.
PROSITE	PS01331. THYMIDYLATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KTHY_BACSU

Accession

Primary (citable) accession number: P37537

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	June 16, 2009
Last modified:	November 24, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)