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P37530 (DGK_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyguanosine kinase

Short name=DGUO kinase
Short name=dGK
EC=2.7.1.113
Gene names
Name:dgk
Synonyms:yaaG
Ordered Locus Names:BSU00150
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an essential role in generating the deoxyribonucleotide precursors dGTP for DNA metabolism. Highly specific toward deoxyguanosine (dGuo) and deoxyinosine (dIno). Only marginal activity is observed with guanosine. UTP is slightly more efficient as phosphate donor than CTP, ATP and GTP. Ref.3

Catalytic activity

ATP + deoxyguanosine = ADP + dGMP.

Enzyme regulation

Inhibited by deoxyguanosine at concentrations above 30 µM only with UTP as phosphate donor. dGTP is a potent competitive inhibitor. Ref.3

Subunit structure

Homodimer. Ref.3

Miscellaneous

Catalysis proceeds by a classical ping-pong bi-bi reaction mechanism.

Sequence similarities

Belongs to the DCK/DGK family.

Biophysicochemical properties

Kinetic parameters:

KM=0.6 µM for deoxyguanosine (with UTP at pH 7.8 and at 37 degrees Celsius) Ref.3

KM=1.7 µM for deoxyguanosine (with CTP at pH 7.8 and at 37 degrees Celsius)

KM=6.5 µM for deoxyguanosine (with ATP at pH 7.8 and at 37 degrees Celsius)

KM=10.4 µM for deoxyguanosine (with GTP at pH 7.8 and at 37 degrees Celsius)

KM=6 µM for UTP (with deoxyguanosine at pH 7.8 and at 37 degrees Celsius)

KM=35 µM for CTP (with deoxyguanosine at pH 7.8 and at 37 degrees Celsius)

KM=36 µM for ATP (with deoxyguanosine at pH 7.8 and at 37 degrees Celsius)

KM=46 µM for GTP (with deoxyguanosine at pH 7.8 and at 37 degrees Celsius)

Vmax=8.1 µmol/min/mg enzyme toward deoxyguanosine (with 0.5 mM UTP at pH 7.8 and at 37 degrees Celsius)

pH dependence:

Optimum pH is around pH 9 (with saturating concentrations of dGuo and UTP). At pH 7.5 and 11.5 more than 80% of maximal activity is still observed. At pH 6.0, 60% activity remains, whereas the enzyme is completely inactive below pH 5.6.

Mass spectrometry

Molecular mass is 24147 Da from positions 1 - 207. Determined by ESI. Ref.3

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 207207Deoxyguanosine kinase
PRO_0000049431

Regions

Nucleotide binding11 – 199ATP By similarity

Sites

Active site861Proton acceptor Potential
Binding site351Substrate By similarity
Binding site471Substrate By similarity
Binding site1471Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P37530 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 0F77C6B31E9AA331

FASTA20724,145
        10         20         30         40         50         60 
MNTAPFIAIE GPIGAGKTTL ATMLSQKFGF PMINEIVEDN PYLDKFYDNI KEWSFQLEMF 

        70         80         90        100        110        120 
FLCHRYKQLE DTSDHFLKKG QPVIADYHIY KNVIFAERTL SPHQLEKYKK IYHLLTDDLP 

       130        140        150        160        170        180 
KPNFIIYIKA SLPTLLHRIE KRGRPFEKKI ETSYLEQLIS DYEVAIKQLQ EADPELTVLT 

       190        200 
VDGDSKDFVL NKSDFERIAA HVKELIV 

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
Ogasawara N., Nakai S., Yoshikawa H.
DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Deoxynucleoside kinases encoded by the yaaG and yaaF genes of Bacillus subtilis. Substrate specificity and kinetic analysis of deoxyguanosine kinase with UTP as the preferred phosphate donor."
Andersen R.B., Neuhard J.
J. Biol. Chem. 276:5518-5524(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MASS SPECTROMETRY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, ENZYME REGULATION, NOMENCLATURE, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D26185 Genomic DNA. Translation: BAA05251.1.
AL009126 Genomic DNA. Translation: CAB11791.1.
PIRS66045.
RefSeqNP_387896.1. NC_000964.3.

3D structure databases

ProteinModelPortalP37530.
SMRP37530. Positions 5-203.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU00150.

Proteomic databases

PaxDbP37530.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB11791; CAB11791; BSU00150.
GeneID937037.
KEGGbsu:BSU00150.
PATRIC18971491. VBIBacSub10457_0016.

Organism-specific databases

GenoListBSU00150. [Micado]

Phylogenomic databases

eggNOGCOG1428.
HOGENOMHOG000030092.
KOK15518.
OMAVESAMTH.
OrthoDBEOG628F9B.
PhylomeDBP37530.

Enzyme and pathway databases

BioCycBSUB:BSU00150-MONOMER.
SABIO-RKP37530.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR002624. Deoxynucleoside_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10513. PTHR10513. 1 hit.
PfamPF01712. dNK. 1 hit.
[Graphical view]
PIRSFPIRSF000705. DNK. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDGK_BACSU
AccessionPrimary (citable) accession number: P37530
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList