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Protein

Deoxyguanosine kinase

Gene

dgk

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in generating the deoxyribonucleotide precursors dGTP for DNA metabolism. Highly specific toward deoxyguanosine (dGuo) and deoxyinosine (dIno). Only marginal activity is observed with guanosine. UTP is slightly more efficient as phosphate donor than CTP, ATP and GTP.1 Publication

Catalytic activityi

ATP + deoxyguanosine = ADP + dGMP.

Enzyme regulationi

Inhibited by deoxyguanosine at concentrations above 30 µM only with UTP as phosphate donor. dGTP is a potent competitive inhibitor.1 Publication

Kineticsi

  1. KM=0.6 µM for deoxyguanosine (with UTP at pH 7.8 and at 37 degrees Celsius)1 Publication
  2. KM=1.7 µM for deoxyguanosine (with CTP at pH 7.8 and at 37 degrees Celsius)1 Publication
  3. KM=6.5 µM for deoxyguanosine (with ATP at pH 7.8 and at 37 degrees Celsius)1 Publication
  4. KM=10.4 µM for deoxyguanosine (with GTP at pH 7.8 and at 37 degrees Celsius)1 Publication
  5. KM=6 µM for UTP (with deoxyguanosine at pH 7.8 and at 37 degrees Celsius)1 Publication
  6. KM=35 µM for CTP (with deoxyguanosine at pH 7.8 and at 37 degrees Celsius)1 Publication
  7. KM=36 µM for ATP (with deoxyguanosine at pH 7.8 and at 37 degrees Celsius)1 Publication
  8. KM=46 µM for GTP (with deoxyguanosine at pH 7.8 and at 37 degrees Celsius)1 Publication
  1. Vmax=8.1 µmol/min/mg enzyme toward deoxyguanosine (with 0.5 mM UTP at pH 7.8 and at 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is around pH 9 (with saturating concentrations of dGuo and UTP). At pH 7.5 and 11.5 more than 80% of maximal activity is still observed. At pH 6.0, 60% activity remains, whereas the enzyme is completely inactive below pH 5.6.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35SubstrateBy similarity1
Binding sitei47SubstrateBy similarity1
Active sitei86Proton acceptorSequence analysis1
Binding sitei147SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 19ATPBy similarity9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU00150-MONOMER.
SABIO-RKP37530.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyguanosine kinase (EC:2.7.1.113)
Short name:
DGUO kinase
Short name:
dGK
Gene namesi
Name:dgk
Synonyms:yaaG
Ordered Locus Names:BSU00150
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000494311 – 207Deoxyguanosine kinaseAdd BLAST207

Proteomic databases

PaxDbiP37530.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100000075.

Structurei

3D structure databases

ProteinModelPortaliP37530.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DCK/DGK family.Curated

Phylogenomic databases

eggNOGiCOG1428. LUCA.
HOGENOMiHOG000030092.
InParanoidiP37530.
KOiK15518.
OMAiGFPMINE.
PhylomeDBiP37530.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DCK/DGK.
IPR031314. DNK_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P37530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTAPFIAIE GPIGAGKTTL ATMLSQKFGF PMINEIVEDN PYLDKFYDNI
60 70 80 90 100
KEWSFQLEMF FLCHRYKQLE DTSDHFLKKG QPVIADYHIY KNVIFAERTL
110 120 130 140 150
SPHQLEKYKK IYHLLTDDLP KPNFIIYIKA SLPTLLHRIE KRGRPFEKKI
160 170 180 190 200
ETSYLEQLIS DYEVAIKQLQ EADPELTVLT VDGDSKDFVL NKSDFERIAA

HVKELIV
Length:207
Mass (Da):24,145
Last modified:October 1, 1994 - v1
Checksum:i0F77C6B31E9AA331
GO

Mass spectrometryi

Molecular mass is 24147 Da from positions 1 - 207. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05251.1.
AL009126 Genomic DNA. Translation: CAB11791.1.
PIRiS66045.
RefSeqiNP_387896.1. NC_000964.3.
WP_003247138.1. NZ_JNCM01000024.1.

Genome annotation databases

EnsemblBacteriaiCAB11791; CAB11791; BSU00150.
GeneIDi937037.
KEGGibsu:BSU00150.
PATRICi18971491. VBIBacSub10457_0016.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05251.1.
AL009126 Genomic DNA. Translation: CAB11791.1.
PIRiS66045.
RefSeqiNP_387896.1. NC_000964.3.
WP_003247138.1. NZ_JNCM01000024.1.

3D structure databases

ProteinModelPortaliP37530.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100000075.

Proteomic databases

PaxDbiP37530.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11791; CAB11791; BSU00150.
GeneIDi937037.
KEGGibsu:BSU00150.
PATRICi18971491. VBIBacSub10457_0016.

Phylogenomic databases

eggNOGiCOG1428. LUCA.
HOGENOMiHOG000030092.
InParanoidiP37530.
KOiK15518.
OMAiGFPMINE.
PhylomeDBiP37530.

Enzyme and pathway databases

BioCyciBSUB:BSU00150-MONOMER.
SABIO-RKP37530.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DCK/DGK.
IPR031314. DNK_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDGK_BACSU
AccessioniPrimary (citable) accession number: P37530
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 5, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Catalysis proceeds by a classical ping-pong bi-bi reaction mechanism.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.