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P37529

- DCK_BACSU

UniProt

P37529 - DCK_BACSU

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Protein

Deoxyadenosine/deoxycytidine kinase

Gene

dck

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Plays an essential role in generating the deoxyribonucleotide precursors dATP AND dCTP for DNA metabolism. The phosphate acceptor specificity is strict toward deoxyadenosine (dAdo) and deoxycytidine (dCyd). The specificity toward the sugar moiety of the nucleoside is less strict. Both 2-deoxyribose, ribose, and arabinose nucleosides are phosphorylated, although the 2-deoxyribonucleosides are preferred. The phosphate donor specificity is dependent on the deoxyribonucleoside substrate, but GTP is efficient with both deoxycytidine and deoxyadenosine. Only nucleoside triphosphates can act as phosphate donors.1 Publication

Catalytic activityi

ATP + deoxyadenosine = ADP + dAMP.
NTP + deoxycytidine = NDP + dCMP.

Enzyme regulationi

Deoxyadenosine inhibits deoxycytidine phosphorylation and deoxycytidine inhibits deoxyadenosine phosphorylation. Deoxyadenosine/deoxycytidine kinase is inhibited by both dATP and dCTP.1 Publication

Kineticsi

  1. KM=5 µM for deoxyadenosine (with GTP at pH 8.5 and at 37 degrees Celsius)1 Publication
  2. KM=5 µM for deoxycytidine (with GTP at pH 8.5 and at 37 degrees Celsius)1 Publication
  3. KM=65 µM for GTP (with deoxyadenosine or deoxycytidine at pH 7.8 and at 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is around pH 8.6. At pH 8.0-9.6 more than 90% of maximal activity is still observed.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401SubstrateBy similarity
Binding sitei52 – 521SubstrateBy similarity
Binding sitei63 – 631SubstrateBy similarity
Active sitei87 – 871Proton acceptorSequence Analysis
Binding sitei88 – 881SubstrateBy similarity
Binding sitei93 – 931SubstrateBy similarity
Binding sitei156 – 1561SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 249ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. deoxyadenosine kinase activity Source: UniProtKB-EC
  3. deoxycytidine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU00140-MONOMER.
MetaCyc:BSU00140-MONOMER.
SABIO-RKP37529.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyadenosine/deoxycytidine kinase (EC:2.7.1.74, EC:2.7.1.76)
Short name:
dAK/dCK
Gene namesi
Name:dck
Synonyms:dak, yaaF
Ordered Locus Names:BSU00140
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU00140. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217Deoxyadenosine/deoxycytidine kinasePRO_0000049430Add
BLAST

Proteomic databases

PaxDbiP37529.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.BSU00140.

Structurei

3D structure databases

ProteinModelPortaliP37529.
SMRiP37529. Positions 6-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DCK/DGK family.Curated

Phylogenomic databases

eggNOGiCOG1428.
HOGENOMiHOG000030092.
InParanoidiP37529.
KOiK15519.
OMAiIYGDVIF.
OrthoDBiEOG628F9B.
PhylomeDBiP37529.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P37529-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEHHIPKNS IITVAGTVGV GKSTLTKTLA KRLGFKTSLE EVDHNPYLEK
60 70 80 90 100
FYHDFERWSF HLQIYFLAER FKEQKTIFEA GGGFVQDRSI YEDTGIFAKM
110 120 130 140 150
HADKGTMSKV DYKTYTSLFE AMVMTPYFPH PDVLIYLEGD LENILNRIEQ
160 170 180 190 200
RGREMELQTS RSYWEEMHTR YENWISGFNA CPVLKLRIED YDLLNDENSI
210
ENIVDQIASV IHDNQKK
Length:217
Mass (Da):25,444
Last modified:October 1, 1994 - v1
Checksum:iB02CDBAA1C518305
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26185 Genomic DNA. Translation: BAA05250.1.
AL009126 Genomic DNA. Translation: CAB11790.1.
PIRiS66044.
RefSeqiNP_387895.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB11790; CAB11790; BSU00140.
GeneIDi936793.
KEGGibsu:BSU00140.
PATRICi18971489. VBIBacSub10457_0015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26185 Genomic DNA. Translation: BAA05250.1 .
AL009126 Genomic DNA. Translation: CAB11790.1 .
PIRi S66044.
RefSeqi NP_387895.1. NC_000964.3.

3D structure databases

ProteinModelPortali P37529.
SMRi P37529. Positions 6-212.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU00140.

Proteomic databases

PaxDbi P37529.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB11790 ; CAB11790 ; BSU00140 .
GeneIDi 936793.
KEGGi bsu:BSU00140.
PATRICi 18971489. VBIBacSub10457_0015.

Organism-specific databases

GenoListi BSU00140. [Micado ]

Phylogenomic databases

eggNOGi COG1428.
HOGENOMi HOG000030092.
InParanoidi P37529.
KOi K15519.
OMAi IYGDVIF.
OrthoDBi EOG628F9B.
PhylomeDBi P37529.

Enzyme and pathway databases

BioCyci BSUB:BSU00140-MONOMER.
MetaCyc:BSU00140-MONOMER.
SABIO-RK P37529.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10513. PTHR10513. 1 hit.
Pfami PF01712. dNK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000705. DNK. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
    Ogasawara N., Nakai S., Yoshikawa H.
    DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Deoxyadenosine/deoxycytidine kinase from Bacillus subtilis. Purification, characterization, and physiological function."
    Moellgaard H.
    J. Biol. Chem. 255:8216-8220(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, ENZYME REGULATION.
  4. "Deoxynucleoside kinases encoded by the yaaG and yaaF genes of Bacillus subtilis. Substrate specificity and kinetic analysis of deoxyguanosine kinase with UTP as the preferred phosphate donor."
    Andersen R.B., Neuhard J.
    J. Biol. Chem. 276:5518-5524(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.

Entry informationi

Entry nameiDCK_BACSU
AccessioniPrimary (citable) accession number: P37529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3