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Protein

Deoxyadenosine/deoxycytidine kinase

Gene

dck

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in generating the deoxyribonucleotide precursors dATP AND dCTP for DNA metabolism. The phosphate acceptor specificity is strict toward deoxyadenosine (dAdo) and deoxycytidine (dCyd). The specificity toward the sugar moiety of the nucleoside is less strict. Both 2-deoxyribose, ribose, and arabinose nucleosides are phosphorylated, although the 2-deoxyribonucleosides are preferred. The phosphate donor specificity is dependent on the deoxyribonucleoside substrate, but GTP is efficient with both deoxycytidine and deoxyadenosine. Only nucleoside triphosphates can act as phosphate donors.1 Publication

Catalytic activityi

ATP + deoxyadenosine = ADP + dAMP.
NTP + deoxycytidine = NDP + dCMP.

Enzyme regulationi

Deoxyadenosine inhibits deoxycytidine phosphorylation and deoxycytidine inhibits deoxyadenosine phosphorylation. Deoxyadenosine/deoxycytidine kinase is inhibited by both dATP and dCTP.1 Publication

Kineticsi

  1. KM=5 µM for deoxyadenosine (with GTP at pH 8.5 and at 37 degrees Celsius)1 Publication
  2. KM=5 µM for deoxycytidine (with GTP at pH 8.5 and at 37 degrees Celsius)1 Publication
  3. KM=65 µM for GTP (with deoxyadenosine or deoxycytidine at pH 7.8 and at 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is around pH 8.6. At pH 8.0-9.6 more than 90% of maximal activity is still observed.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401SubstrateBy similarity
    Binding sitei52 – 521SubstrateBy similarity
    Binding sitei63 – 631SubstrateBy similarity
    Active sitei87 – 871Proton acceptorSequence Analysis
    Binding sitei88 – 881SubstrateBy similarity
    Binding sitei93 – 931SubstrateBy similarity
    Binding sitei156 – 1561SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 249ATPBy similarity

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU00140-MONOMER.
    MetaCyc:BSU00140-MONOMER.
    SABIO-RKP37529.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyadenosine/deoxycytidine kinase (EC:2.7.1.74, EC:2.7.1.76)
    Short name:
    dAK/dCK
    Gene namesi
    Name:dck
    Synonyms:dak, yaaF
    Ordered Locus Names:BSU00140
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570 Componenti: Chromosome

    Organism-specific databases

    GenoListiBSU00140. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 217217Deoxyadenosine/deoxycytidine kinasePRO_0000049430Add
    BLAST

    Proteomic databases

    PaxDbiP37529.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100000070.

    Structurei

    3D structure databases

    ProteinModelPortaliP37529.
    SMRiP37529. Positions 6-212.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DCK/DGK family.Curated

    Phylogenomic databases

    eggNOGiCOG1428.
    HOGENOMiHOG000030092.
    InParanoidiP37529.
    KOiK15519.
    OMAiTRYENWI.
    OrthoDBiEOG628F9B.
    PhylomeDBiP37529.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10513. PTHR10513. 1 hit.
    PfamiPF01712. dNK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000705. DNK. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P37529-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKEHHIPKNS IITVAGTVGV GKSTLTKTLA KRLGFKTSLE EVDHNPYLEK
    60 70 80 90 100
    FYHDFERWSF HLQIYFLAER FKEQKTIFEA GGGFVQDRSI YEDTGIFAKM
    110 120 130 140 150
    HADKGTMSKV DYKTYTSLFE AMVMTPYFPH PDVLIYLEGD LENILNRIEQ
    160 170 180 190 200
    RGREMELQTS RSYWEEMHTR YENWISGFNA CPVLKLRIED YDLLNDENSI
    210
    ENIVDQIASV IHDNQKK
    Length:217
    Mass (Da):25,444
    Last modified:October 1, 1994 - v1
    Checksum:iB02CDBAA1C518305
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D26185 Genomic DNA. Translation: BAA05250.1.
    AL009126 Genomic DNA. Translation: CAB11790.1.
    PIRiS66044.
    RefSeqiNP_387895.1. NC_000964.3.
    WP_003226792.1. NZ_JNCM01000024.1.

    Genome annotation databases

    EnsemblBacteriaiCAB11790; CAB11790; BSU00140.
    GeneIDi936793.
    KEGGibsu:BSU00140.
    PATRICi18971489. VBIBacSub10457_0015.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D26185 Genomic DNA. Translation: BAA05250.1.
    AL009126 Genomic DNA. Translation: CAB11790.1.
    PIRiS66044.
    RefSeqiNP_387895.1. NC_000964.3.
    WP_003226792.1. NZ_JNCM01000024.1.

    3D structure databases

    ProteinModelPortaliP37529.
    SMRiP37529. Positions 6-212.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100000070.

    Proteomic databases

    PaxDbiP37529.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB11790; CAB11790; BSU00140.
    GeneIDi936793.
    KEGGibsu:BSU00140.
    PATRICi18971489. VBIBacSub10457_0015.

    Organism-specific databases

    GenoListiBSU00140. [Micado]

    Phylogenomic databases

    eggNOGiCOG1428.
    HOGENOMiHOG000030092.
    InParanoidiP37529.
    KOiK15519.
    OMAiTRYENWI.
    OrthoDBiEOG628F9B.
    PhylomeDBiP37529.

    Enzyme and pathway databases

    BioCyciBSUB:BSU00140-MONOMER.
    MetaCyc:BSU00140-MONOMER.
    SABIO-RKP37529.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10513. PTHR10513. 1 hit.
    PfamiPF01712. dNK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000705. DNK. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
      Ogasawara N., Nakai S., Yoshikawa H.
      DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Deoxyadenosine/deoxycytidine kinase from Bacillus subtilis. Purification, characterization, and physiological function."
      Moellgaard H.
      J. Biol. Chem. 255:8216-8220(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, ENZYME REGULATION.
    4. "Deoxynucleoside kinases encoded by the yaaG and yaaF genes of Bacillus subtilis. Substrate specificity and kinetic analysis of deoxyguanosine kinase with UTP as the preferred phosphate donor."
      Andersen R.B., Neuhard J.
      J. Biol. Chem. 276:5518-5524(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.

    Entry informationi

    Entry nameiDCK_BACSU
    AccessioniPrimary (citable) accession number: P37529
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: June 24, 2015
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.