P37478 (YYCF_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein YycF

Gene names

Name:	yycF
Ordered Locus Names:	BSU40410

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system YycG/YycF involved in the regulation of the ftsAZ operon, the yocH, ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Probably phosphorylates YycF. Binds to the ftsAZ P1 promoter sequence in vitro. YycF has been shown to directly bind to the regulatory regions of yocH, ykvT, tagAB/tagDEF. Activates cwlO, lytE and ydjM and represses yoeB and yjeA. Ref.4 Ref.5 Ref.8
Subunit structure	Homodimer. Ref.6
Subcellular location	Cytoplasm Probable.
Developmental stage	Expressed during exponential growth and shut down at the entry into stationary phase.
Post-translational modification	Phosphorylated by YycG Probable.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 235

235

Transcriptional regulatory protein YycF

PRO_0000081399

Regions

Domain

4 – 117

114

Response regulatory

Amino acid modifications

Modified residue

53

1

4-aspartylphosphate Probable

Experimental info

Mutagenesis

53

1

D → H: Constitutively active. Ref.5

Mutagenesis

215

1

H → P in JH17041; thermosensitive; decrease in dimerization and DNA-binding. Ref.4 Ref.6

Secondary structure

1

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235

Helix Strand Turn

Details...

Beta strand

135 – 137

3

Beta strand

140 – 143

4

Turn

144 – 147

4

Beta strand

148 – 151

4

Helix

160 – 171

12

Turn

172 – 174

3

Helix

179 – 187

9

Helix

196 – 209

14

Beta strand

217 – 222

6

Turn

223 – 225

3

Beta strand

226 – 229

4

Sequences

	Sequence		Length	Mass (Da)
	P37478 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 201BC21CBB5EC6B4	FASTA	235	27,226
	10 20 30 40 50 60 MDKKILVVDD EKPIADILEF NLRKEGYEVH CAHDGNEAVE MVEELQPDLI LLDIMLPNKD 70 80 90 100 110 120 GVEVCREVRK KYDMPIIMLT AKDSEIDKVI GLEIGADDYV TKPFSTRELL ARVKANLRRQ 130 140 150 160 170 180 LTTAPAEEEP SSNEIHIGSL VIFPDAYVVS KRDETIELTH REFELLHYLA KHIGQVMTRE 190 200 210 220 230 HLLQTVWGYD YFGDVRTVDV TVRRLREKIE DNPSHPNWIV TRRGVGYYLR NPEQD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"Sequence analysis of the 36-kb region between gntZ and trnY genes of Bacillus subtilis genome." Kasahara Y., Nakai S., Ogasawara N. DNA Res. 4:155-159(1997) [PubMed: 9205843] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy." Fabret C., Hoch J.A. J. Bacteriol. 180:6375-6383(1998) [PubMed: 9829949] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF HIS-215. Strain: 168 / JH642.
[5]	"The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis." Fukuchi K., Kasahara Y., Asai K., Kobayashi K., Moriya S., Ogasawara N. Microbiology 146:1573-1583(2000) [PubMed: 10878122] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-53. Strain: 168.
[6]	"Molecular characterization of the essential response regulator protein YycF in Bacillus subtilis." Watanabe T., Hashimoto Y., Umemoto Y., Tatebe D., Furuta E., Fukamizo T., Yamamoto K., Utsumi R. J. Mol. Microbiol. Biotechnol. 6:155-163(2003) [PubMed: 15153768] [Abstract] Cited for: MUTAGENESIS OF HIS-215, SUBUNIT.
[7]	"Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach." Howell A., Dubrac S., Andersen K.K., Noone D., Fert J., Msadek T., Devine K. Mol. Microbiol. 49:1639-1655(2003) [PubMed: 12950927] [Abstract] Cited for: STUDY OF THE YYCF/YYCG REGULON.
[8]	"The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis." Bisicchia P., Noone D., Lioliou E., Howell A., Quigley S., Jensen T., Jarmer H., Devine K.M. Mol. Microbiol. 65:180-200(2007) [PubMed: 17581128] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D26185 Genomic DNA. Translation: BAA05173.1.
D78193 Genomic DNA. Translation: BAA11300.1.
AL009126 Genomic DNA. Translation: CAB16078.1.

PIR

S65967.

RefSeq

NP_391921.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D1V	X-ray	2.40	A	128-235	[»]
2ZWM	X-ray	2.04	A/B	1-122	[»]
3F6P	X-ray	1.95	A	1-120	[»]

ProteinModelPortal

P37478.

SMR

P37478. Positions 1-120, 131-233.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000001518; EBBACP00000001518; EBBACG00000001516.

GeneID

937776.

GenomeReviews

Gene locus BSU40410 in contig AL009126_GR.

KEGG

bsu:BSU40410.

NMPDR

fig|224308.1.peg.4047.

PATRIC

18980160. VBIBacSub10457_4242.

Organism-specific databases

GenoList

BSU40410.

Phylogenomic databases

GeneTree

EBGT00070000031753.

HOGENOM

HBG753323.

OMA

KPISDIM.

PhylomeDB

P37478.

ProtClustDB

CLSK2391287.

Enzyme and pathway databases

BioCyc

BSUB:BSU40410-MONOMER.

Family and domain databases

InterPro

IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

KO

K07668.

Pfam

PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]

SMART

SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]

SUPFAM

SSF52172. CheY_like. 1 hit.

PROSITE

PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

YYCF_BACSU

Accession

Primary (citable) accession number: P37478

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program