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P37478

- YYCF_BACSU

UniProt

P37478 - YYCF_BACSU

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Protein

Transcriptional regulatory protein YycF

Gene

yycF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system YycG/YycF involved in the regulation of the ftsAZ operon, the yocH, ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Probably phosphorylates YycF. Binds to the ftsAZ P1 promoter sequence in vitro. YycF has been shown to directly bind to the regulatory regions of yocH, ykvT, tagAB/tagDEF. Activates cwlO, lytE and ydjM and represses yoeB and yjeA.3 Publications

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. phosphorelay signal transduction system Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: CACAO
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU40410-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein YycF
Gene namesi
Name:yycF
Ordered Locus Names:BSU40410
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU40410.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi53 – 531D → H: Constitutively active. 1 Publication
Mutagenesisi215 – 2151H → P in JH17041; thermosensitive; decrease in dimerization and DNA-binding. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 235235Transcriptional regulatory protein YycFPRO_0000081399Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 5314-aspartylphosphateCurated

Post-translational modificationi

Phosphorylated by YycG.Curated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP37478.

Expressioni

Developmental stagei

Expressed during exponential growth and shut down at the entry into stationary phase.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.BSU40410.

Structurei

Secondary structure

1
235
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85Combined sources
Helixi12 – 2413Combined sources
Beta strandi28 – 347Combined sources
Helixi35 – 439Combined sources
Beta strandi48 – 536Combined sources
Turni57 – 593Combined sources
Helixi60 – 6910Combined sources
Beta strandi76 – 838Combined sources
Helixi85 – 939Combined sources
Beta strandi98 – 1036Combined sources
Helixi106 – 11712Combined sources
Beta strandi135 – 1373Combined sources
Beta strandi140 – 1434Combined sources
Turni144 – 1474Combined sources
Beta strandi148 – 1514Combined sources
Helixi160 – 17112Combined sources
Turni172 – 1743Combined sources
Helixi179 – 1879Combined sources
Helixi196 – 20914Combined sources
Beta strandi217 – 2226Combined sources
Turni223 – 2253Combined sources
Beta strandi226 – 2294Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D1VX-ray2.40A128-235[»]
2ZWMX-ray2.04A/B1-122[»]
3F6PX-ray1.95A1-120[»]
ProteinModelPortaliP37478.
SMRiP37478. Positions 1-120, 131-233.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37478.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 117114Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034819.
InParanoidiP37478.
KOiK07668.
OMAiHEILSYG.
OrthoDBiEOG6G4VQG.
PhylomeDBiP37478.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37478-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDKKILVVDD EKPIADILEF NLRKEGYEVH CAHDGNEAVE MVEELQPDLI
60 70 80 90 100
LLDIMLPNKD GVEVCREVRK KYDMPIIMLT AKDSEIDKVI GLEIGADDYV
110 120 130 140 150
TKPFSTRELL ARVKANLRRQ LTTAPAEEEP SSNEIHIGSL VIFPDAYVVS
160 170 180 190 200
KRDETIELTH REFELLHYLA KHIGQVMTRE HLLQTVWGYD YFGDVRTVDV
210 220 230
TVRRLREKIE DNPSHPNWIV TRRGVGYYLR NPEQD
Length:235
Mass (Da):27,226
Last modified:October 1, 1994 - v1
Checksum:i201BC21CBB5EC6B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05173.1.
D78193 Genomic DNA. Translation: BAA11300.1.
AL009126 Genomic DNA. Translation: CAB16078.1.
PIRiS65967.
RefSeqiNP_391921.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB16078; CAB16078; BSU40410.
GeneIDi937776.
KEGGibsu:BSU40410.
PATRICi18980160. VBIBacSub10457_4242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05173.1 .
D78193 Genomic DNA. Translation: BAA11300.1 .
AL009126 Genomic DNA. Translation: CAB16078.1 .
PIRi S65967.
RefSeqi NP_391921.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2D1V X-ray 2.40 A 128-235 [» ]
2ZWM X-ray 2.04 A/B 1-122 [» ]
3F6P X-ray 1.95 A 1-120 [» ]
ProteinModelPortali P37478.
SMRi P37478. Positions 1-120, 131-233.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU40410.

Proteomic databases

PaxDbi P37478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB16078 ; CAB16078 ; BSU40410 .
GeneIDi 937776.
KEGGi bsu:BSU40410.
PATRICi 18980160. VBIBacSub10457_4242.

Organism-specific databases

GenoListi BSU40410.

Phylogenomic databases

eggNOGi COG0745.
HOGENOMi HOG000034819.
InParanoidi P37478.
KOi K07668.
OMAi HEILSYG.
OrthoDBi EOG6G4VQG.
PhylomeDBi P37478.

Enzyme and pathway databases

BioCyci BSUB:BSU40410-MONOMER.

Miscellaneous databases

EvolutionaryTracei P37478.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view ]
SMARTi SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view ]
SUPFAMi SSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
    Ogasawara N., Nakai S., Yoshikawa H.
    DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "Sequence analysis of the 36-kb region between gntZ and trnY genes of Bacillus subtilis genome."
    Kasahara Y., Nakai S., Ogasawara N.
    DNA Res. 4:155-159(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy."
    Fabret C., Hoch J.A.
    J. Bacteriol. 180:6375-6383(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF HIS-215.
    Strain: 168 / JH642.
  5. "The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis."
    Fukuchi K., Kasahara Y., Asai K., Kobayashi K., Moriya S., Ogasawara N.
    Microbiology 146:1573-1583(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-53.
    Strain: 168.
  6. "Molecular characterization of the essential response regulator protein YycF in Bacillus subtilis."
    Watanabe T., Hashimoto Y., Umemoto Y., Tatebe D., Furuta E., Fukamizo T., Yamamoto K., Utsumi R.
    J. Mol. Microbiol. Biotechnol. 6:155-163(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-215, SUBUNIT.
  7. "Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach."
    Howell A., Dubrac S., Andersen K.K., Noone D., Fert J., Msadek T., Devine K.
    Mol. Microbiol. 49:1639-1655(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STUDY OF THE YYCF/YYCG REGULON.
  8. "The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis."
    Bisicchia P., Noone D., Lioliou E., Howell A., Quigley S., Jensen T., Jarmer H., Devine K.M.
    Mol. Microbiol. 65:180-200(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiYYCF_BACSU
AccessioniPrimary (citable) accession number: P37478
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 26, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3