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P37478

- YYCF_BACSU

UniProt

P37478 - YYCF_BACSU

Protein

Transcriptional regulatory protein YycF

Gene

yycF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Member of the two-component regulatory system YycG/YycF involved in the regulation of the ftsAZ operon, the yocH, ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Probably phosphorylates YycF. Binds to the ftsAZ P1 promoter sequence in vitro. YycF has been shown to directly bind to the regulatory regions of yocH, ykvT, tagAB/tagDEF. Activates cwlO, lytE and ydjM and represses yoeB and yjeA.3 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. phosphorelay response regulator activity Source: InterPro

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: CACAO
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU40410-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional regulatory protein YycF
    Gene namesi
    Name:yycF
    Ordered Locus Names:BSU40410
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU40410.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi53 – 531D → H: Constitutively active. 1 Publication
    Mutagenesisi215 – 2151H → P in JH17041; thermosensitive; decrease in dimerization and DNA-binding. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 235235Transcriptional regulatory protein YycFPRO_0000081399Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei53 – 5314-aspartylphosphateCurated

    Post-translational modificationi

    Phosphorylated by YycG.Curated

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP37478.

    Expressioni

    Developmental stagei

    Expressed during exponential growth and shut down at the entry into stationary phase.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi224308.BSU40410.

    Structurei

    Secondary structure

    1
    235
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 85
    Helixi12 – 2413
    Beta strandi28 – 347
    Helixi35 – 439
    Beta strandi48 – 536
    Turni57 – 593
    Helixi60 – 6910
    Beta strandi76 – 838
    Helixi85 – 939
    Beta strandi98 – 1036
    Helixi106 – 11712
    Beta strandi135 – 1373
    Beta strandi140 – 1434
    Turni144 – 1474
    Beta strandi148 – 1514
    Helixi160 – 17112
    Turni172 – 1743
    Helixi179 – 1879
    Helixi196 – 20914
    Beta strandi217 – 2226
    Turni223 – 2253
    Beta strandi226 – 2294

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2D1VX-ray2.40A128-235[»]
    2ZWMX-ray2.04A/B1-122[»]
    3F6PX-ray1.95A1-120[»]
    ProteinModelPortaliP37478.
    SMRiP37478. Positions 1-120, 131-233.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP37478.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 117114Response regulatoryPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0745.
    HOGENOMiHOG000034819.
    KOiK07668.
    OMAiHEILSYG.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiP37478.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P37478-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKKILVVDD EKPIADILEF NLRKEGYEVH CAHDGNEAVE MVEELQPDLI    50
    LLDIMLPNKD GVEVCREVRK KYDMPIIMLT AKDSEIDKVI GLEIGADDYV 100
    TKPFSTRELL ARVKANLRRQ LTTAPAEEEP SSNEIHIGSL VIFPDAYVVS 150
    KRDETIELTH REFELLHYLA KHIGQVMTRE HLLQTVWGYD YFGDVRTVDV 200
    TVRRLREKIE DNPSHPNWIV TRRGVGYYLR NPEQD 235
    Length:235
    Mass (Da):27,226
    Last modified:October 1, 1994 - v1
    Checksum:i201BC21CBB5EC6B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26185 Genomic DNA. Translation: BAA05173.1.
    D78193 Genomic DNA. Translation: BAA11300.1.
    AL009126 Genomic DNA. Translation: CAB16078.1.
    PIRiS65967.
    RefSeqiNP_391921.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB16078; CAB16078; BSU40410.
    GeneIDi937776.
    KEGGibsu:BSU40410.
    PATRICi18980160. VBIBacSub10457_4242.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26185 Genomic DNA. Translation: BAA05173.1 .
    D78193 Genomic DNA. Translation: BAA11300.1 .
    AL009126 Genomic DNA. Translation: CAB16078.1 .
    PIRi S65967.
    RefSeqi NP_391921.1. NC_000964.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2D1V X-ray 2.40 A 128-235 [» ]
    2ZWM X-ray 2.04 A/B 1-122 [» ]
    3F6P X-ray 1.95 A 1-120 [» ]
    ProteinModelPortali P37478.
    SMRi P37478. Positions 1-120, 131-233.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU40410.

    Proteomic databases

    PaxDbi P37478.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB16078 ; CAB16078 ; BSU40410 .
    GeneIDi 937776.
    KEGGi bsu:BSU40410.
    PATRICi 18980160. VBIBacSub10457_4242.

    Organism-specific databases

    GenoListi BSU40410.

    Phylogenomic databases

    eggNOGi COG0745.
    HOGENOMi HOG000034819.
    KOi K07668.
    OMAi HEILSYG.
    OrthoDBi EOG6G4VQG.
    PhylomeDBi P37478.

    Enzyme and pathway databases

    BioCyci BSUB:BSU40410-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P37478.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
      Ogasawara N., Nakai S., Yoshikawa H.
      DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "Sequence analysis of the 36-kb region between gntZ and trnY genes of Bacillus subtilis genome."
      Kasahara Y., Nakai S., Ogasawara N.
      DNA Res. 4:155-159(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy."
      Fabret C., Hoch J.A.
      J. Bacteriol. 180:6375-6383(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF HIS-215.
      Strain: 168 / JH642.
    5. "The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis."
      Fukuchi K., Kasahara Y., Asai K., Kobayashi K., Moriya S., Ogasawara N.
      Microbiology 146:1573-1583(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASP-53.
      Strain: 168.
    6. "Molecular characterization of the essential response regulator protein YycF in Bacillus subtilis."
      Watanabe T., Hashimoto Y., Umemoto Y., Tatebe D., Furuta E., Fukamizo T., Yamamoto K., Utsumi R.
      J. Mol. Microbiol. Biotechnol. 6:155-163(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF HIS-215, SUBUNIT.
    7. "Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach."
      Howell A., Dubrac S., Andersen K.K., Noone D., Fert J., Msadek T., Devine K.
      Mol. Microbiol. 49:1639-1655(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STUDY OF THE YYCF/YYCG REGULON.
    8. "The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis."
      Bisicchia P., Noone D., Lioliou E., Howell A., Quigley S., Jensen T., Jarmer H., Devine K.M.
      Mol. Microbiol. 65:180-200(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiYYCF_BACSU
    AccessioniPrimary (citable) accession number: P37478
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3