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Protein

Transcriptional regulatory protein WalR

Gene

walR

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system WalK/WalR involved in the regulation of the ftsAZ operon, the yocH, ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Binds to the ftsAZ P1 promoter sequence in vitro. WalR has been shown to directly bind to the regulatory regions of yocH, ykvT, tagAB/tagDEF. Activates cwlO, lytE and ydjM and represses yoeB and yjeA.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi132 – 231OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST100

GO - Molecular functioni

GO - Biological processi

  • phosphorelay signal transduction system Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: CACAO
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU40410-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein WalRCurated
Gene namesi
Name:walRBy similarityImported
Synonyms:yycF
Ordered Locus Names:BSU40410
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi53D → H: Constitutively active. 1 Publication1
Mutagenesisi215H → P in JH17041; thermosensitive; decrease in dimerization and DNA-binding. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000813991 – 235Transcriptional regulatory protein WalRAdd BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei534-aspartylphosphateCurated1

Post-translational modificationi

Phosphorylated by WalK.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP37478.

Expressioni

Developmental stagei

Expressed during exponential growth and shut down at the entry into stationary phase.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021806.

Structurei

Secondary structure

1235
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi12 – 24Combined sources13
Beta strandi28 – 34Combined sources7
Helixi35 – 43Combined sources9
Beta strandi48 – 53Combined sources6
Turni57 – 59Combined sources3
Helixi60 – 69Combined sources10
Beta strandi76 – 83Combined sources8
Helixi85 – 93Combined sources9
Beta strandi98 – 103Combined sources6
Helixi106 – 117Combined sources12
Beta strandi135 – 137Combined sources3
Beta strandi140 – 143Combined sources4
Turni144 – 147Combined sources4
Beta strandi148 – 151Combined sources4
Helixi160 – 171Combined sources12
Turni172 – 174Combined sources3
Helixi179 – 187Combined sources9
Helixi196 – 209Combined sources14
Beta strandi217 – 222Combined sources6
Turni223 – 225Combined sources3
Beta strandi226 – 229Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1VX-ray2.40A128-235[»]
2ZWMX-ray2.04A/B1-122[»]
3F6PX-ray1.95A1-120[»]
ProteinModelPortaliP37478.
SMRiP37478.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37478.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 117Response regulatoryPROSITE-ProRule annotationAdd BLAST114

Sequence similaritiesi

Contains 1 OmpR/PhoB-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CK6. Bacteria.
COG0745. LUCA.
HOGENOMiHOG000034819.
InParanoidiP37478.
KOiK07668.
OMAiCARIRQK.
PhylomeDBiP37478.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKILVVDD EKPIADILEF NLRKEGYEVH CAHDGNEAVE MVEELQPDLI
60 70 80 90 100
LLDIMLPNKD GVEVCREVRK KYDMPIIMLT AKDSEIDKVI GLEIGADDYV
110 120 130 140 150
TKPFSTRELL ARVKANLRRQ LTTAPAEEEP SSNEIHIGSL VIFPDAYVVS
160 170 180 190 200
KRDETIELTH REFELLHYLA KHIGQVMTRE HLLQTVWGYD YFGDVRTVDV
210 220 230
TVRRLREKIE DNPSHPNWIV TRRGVGYYLR NPEQD
Length:235
Mass (Da):27,226
Last modified:October 1, 1994 - v1
Checksum:i201BC21CBB5EC6B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05173.1.
D78193 Genomic DNA. Translation: BAA11300.1.
AL009126 Genomic DNA. Translation: CAB16078.1.
PIRiS65967.
RefSeqiNP_391921.1. NC_000964.3.
WP_003244363.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB16078; CAB16078; BSU40410.
GeneIDi11241635.
937776.
KEGGibsu:BSU40410.
PATRICi18980160. VBIBacSub10457_4242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05173.1.
D78193 Genomic DNA. Translation: BAA11300.1.
AL009126 Genomic DNA. Translation: CAB16078.1.
PIRiS65967.
RefSeqiNP_391921.1. NC_000964.3.
WP_003244363.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1VX-ray2.40A128-235[»]
2ZWMX-ray2.04A/B1-122[»]
3F6PX-ray1.95A1-120[»]
ProteinModelPortaliP37478.
SMRiP37478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021806.

Proteomic databases

PaxDbiP37478.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB16078; CAB16078; BSU40410.
GeneIDi11241635.
937776.
KEGGibsu:BSU40410.
PATRICi18980160. VBIBacSub10457_4242.

Phylogenomic databases

eggNOGiENOG4105CK6. Bacteria.
COG0745. LUCA.
HOGENOMiHOG000034819.
InParanoidiP37478.
KOiK07668.
OMAiCARIRQK.
PhylomeDBiP37478.

Enzyme and pathway databases

BioCyciBSUB:BSU40410-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37478.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWALR_BACSU
AccessioniPrimary (citable) accession number: P37478
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.