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Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi410 – 4101Magnesium 1By similarity
Metal bindingi417 – 4171Magnesium 1By similarity
Metal bindingi417 – 4171Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU00820-MONOMER.
SABIO-RKP37477.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:lysS
Ordered Locus Names:BSU00820
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Lysine--tRNA ligasePRO_0000152600Add
BLAST

Proteomic databases

PaxDbiP37477.
PRIDEiP37477.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP37477. 1 interaction.
MINTiMINT-8367113.
STRINGi224308.Bsubs1_010100000415.

Structurei

3D structure databases

ProteinModelPortaliP37477.
SMRiP37477. Positions 10-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CRK. Bacteria.
COG1190. LUCA.
HOGENOMiHOG000236578.
InParanoidiP37477.
KOiK04567.
OMAiDMMNLTE.
OrthoDBiEOG69PQ2M.
PhylomeDBiP37477.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF039101. LysRS2. 1 hit.
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQEEHNHEE LNDQLQVRRD KMNQLRDNGI DPFGARFERT HQSQEVISAY
60 70 80 90 100
QDLTKEELEE KAIEVTIAGR MMTKRGKGKA GFAHLQDLEG QIQIYVRKDS
110 120 130 140 150
VGDDQYEIFK SSDLGDLIGV TGKVFKTNVG ELSVKATSFE LLTKALRPLP
160 170 180 190 200
DKYHGLKDVE QRYRQRYLDL IVNPDSKHTF ITRSKIIQAM RRYLDDHGYL
210 220 230 240 250
EVETPTMHSI PGGASARPFI THHNALDIPL YMRIAIELHL KRLIVGGLEK
260 270 280 290 300
VYEIGRVFRN EGVSTRHNPE FTMIELYEAY ADYKDIMSLT ENLVAHIAQE
310 320 330 340 350
VLGTTTIQYG EEQIDLKPEW KRIHMVDAVK EATGVDFWEE VTVEQAREYA
360 370 380 390 400
KEHEVEIKDS MTVGHIINEF FEQKIEETLI QPTFIYGHPV EISPLAKKNP
410 420 430 440 450
EDPRFTDRFE LFIVGREHAN AFTELNDPID QRERFEAQLK EREAGNDEAH
460 470 480 490
LMDEDFVEAL EYGMPPTGGL GIGIDRLVML LTNAPSIRDV LLFPQMRQR
Length:499
Mass (Da):57,539
Last modified:October 1, 1994 - v1
Checksum:i9D082E784473EFE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05316.1.
AL009126 Genomic DNA. Translation: CAB11858.1.
PIRiS66111.
RefSeqiNP_387963.1. NC_000964.3.
WP_003242743.1. NZ_JNCM01000028.1.

Genome annotation databases

EnsemblBacteriaiCAB11858; CAB11858; BSU00820.
GeneIDi936911.
KEGGibsu:BSU00820.
PATRICi18971639. VBIBacSub10457_0083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05316.1.
AL009126 Genomic DNA. Translation: CAB11858.1.
PIRiS66111.
RefSeqiNP_387963.1. NC_000964.3.
WP_003242743.1. NZ_JNCM01000028.1.

3D structure databases

ProteinModelPortaliP37477.
SMRiP37477. Positions 10-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP37477. 1 interaction.
MINTiMINT-8367113.
STRINGi224308.Bsubs1_010100000415.

Proteomic databases

PaxDbiP37477.
PRIDEiP37477.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11858; CAB11858; BSU00820.
GeneIDi936911.
KEGGibsu:BSU00820.
PATRICi18971639. VBIBacSub10457_0083.

Phylogenomic databases

eggNOGiENOG4105CRK. Bacteria.
COG1190. LUCA.
HOGENOMiHOG000236578.
InParanoidiP37477.
KOiK04567.
OMAiDMMNLTE.
OrthoDBiEOG69PQ2M.
PhylomeDBiP37477.

Enzyme and pathway databases

BioCyciBSUB:BSU00820-MONOMER.
SABIO-RKP37477.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF039101. LysRS2. 1 hit.
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
    Ogasawara N., Nakai S., Yoshikawa H.
    DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiSYK_BACSU
AccessioniPrimary (citable) accession number: P37477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: February 17, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.