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P37475

- SP2E_BACSU

UniProt

P37475 - SP2E_BACSU

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Protein

Stage II sporulation protein E

Gene

spoIIE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates SpoIIAA by dephosphorylation.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Manganese.

GO - Molecular functioni

  1. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Sporulation

Enzyme and pathway databases

BioCyciBSUB:BSU00640-MONOMER.

Protein family/group databases

PptaseDBiP3D0406130.

Names & Taxonomyi

Protein namesi
Recommended name:
Stage II sporulation protein E (EC:3.1.3.16)
Alternative name(s):
Stage II sporulation protein H
Gene namesi
Name:spoIIE
Synonyms:spoIIH
Ordered Locus Names:BSU00640
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU00640. [Micado]

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Polar septum.

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
  3. prospore membrane Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 827827Stage II sporulation protein EPRO_0000057794Add
BLAST

Proteomic databases

PaxDbiP37475.

Interactioni

Protein-protein interaction databases

MINTiMINT-126037.
STRINGi224308.BSU00640.

Structurei

Secondary structure

1
827
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi592 – 6009
Beta strandi611 – 6177
Turni618 – 6203
Beta strandi621 – 6277
Beta strandi637 – 6404
Helixi642 – 65312
Helixi657 – 66812
Beta strandi673 – 6753
Beta strandi680 – 6867
Turni687 – 6893
Beta strandi691 – 6988
Beta strandi702 – 7065
Beta strandi709 – 7124
Beta strandi728 – 7347
Beta strandi740 – 7445
Helixi746 – 7494
Beta strandi752 – 7543
Helixi758 – 76710
Helixi774 – 78815
Beta strandi797 – 80610

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3T91X-ray2.64A/B590-827[»]
3T9QX-ray2.76A/B590-827[»]
ProteinModelPortaliP37475.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini341 – 827487CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei49 – 6921HelicalSequence AnalysisAdd
BLAST
Transmembranei71 – 9121HelicalSequence AnalysisAdd
BLAST
Transmembranei116 – 13621HelicalSequence AnalysisAdd
BLAST
Transmembranei142 – 16221HelicalSequence AnalysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence AnalysisAdd
BLAST
Transmembranei206 – 22621HelicalSequence AnalysisAdd
BLAST
Transmembranei247 – 26721HelicalSequence AnalysisAdd
BLAST
Transmembranei269 – 28921HelicalSequence AnalysisAdd
BLAST
Transmembranei299 – 31921HelicalSequence AnalysisAdd
BLAST
Transmembranei320 – 34021HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini605 – 827223PP2C-likeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi799 – 8024Poly-Val

Sequence similaritiesi

Contains 1 PP2C-like domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2208.
HOGENOMiHOG000082091.
InParanoidiP37475.
KOiK06382.
OMAiDGMGNGE.
OrthoDBiEOG6JMMQ9.
PhylomeDBiP37475.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR014221. Spore_II_E.
[Graphical view]
PfamiPF07228. SpoIIE. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
TIGRFAMsiTIGR02865. spore_II_E. 1 hit.

Sequencei

Sequence statusi: Complete.

P37475 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKAERRVNG PMAGQALEKL QSFFNRGTKL VTHHLHSLFF YKGFIYVVIG
60 70 80 90 100
FLLGRAFILS EVLPFALPFF GAMLLIRRDK AFYAVLAVLA GALTISPKHS
110 120 130 140 150
LLILAALLAF FVFSKVAAFI TDDRVKALPI VVFFSMAAAR AGFVYAQNGV
160 170 180 190 200
FTTYDYVMAI VEAGLSFILT LIFLQSLPIF TVKKVKQSLK IEEIICFMIL
210 220 230 240 250
IASVLTGLAG LSYQGMQAEH ILARYVVLSF SFIGGASIGC TVGVVTGLIL
260 270 280 290 300
GLANIGNLYQ MSLLAFSGLL GGLLKEGKKA GAAIGLIVGS LLISLYGEGS
310 320 330 340 350
AGLMTTLYES LIAVCLFLLT PQSITRKVAR YIPGTVEHLQ EQQQYARKIR
360 370 380 390 400
DVTAQKVDQF SNVFHALSES FATFYQASDE QTDDSEVDLF LSKITEHSCQ
410 420 430 440 450
TCYKKNRCWV QNFDKTYDLM KQVMLETEEK EYASNRRLKK EFQQYCSKSK
460 470 480 490 500
QVEELIEDEL AHHHAHLTLK KKVQDSRRLV AEQLLGVSEV MADFSREIKR
510 520 530 540 550
EREQHFLQEE QIIEALQHFG IEIQHVEIYS LEQGNIDIEM TIPFSGHGES
560 570 580 590 600
EKIIAPMLSD ILEEQILVKA EQHSPHPNGY SHVAFGSTKS YRVSTGAAHA
610 620 630 640 650
AKGGGLVSGD SYSMMELGAR KYAAAISDGM GNGARAHFES NETIKLLEKI
660 670 680 690 700
LESGIDEKIA IKTINSILSL RTTDEIYSTL DLSIIDLQDA SCKFLKVGST
710 720 730 740 750
PSFIKRGDQV MKVQASNLPI GIINEFDVEV VSEQLKAGDL LIMMSDGIFE
760 770 780 790 800
GPKHVENHDL WMKRKMKGLK TNDPQEIADL LMEEVIRTRS GQIEDDMTVV
810 820
VVRIDHNTPK WASIPVPAIF QNKQEIS
Length:827
Mass (Da):91,969
Last modified:October 1, 1994 - v1
Checksum:i33EA3A81935B407B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26185 Genomic DNA. Translation: BAA05299.1.
U26835 Genomic DNA. Translation: AAB58073.1.
AL009126 Genomic DNA. Translation: CAB11840.1.
M29403 Genomic DNA. Translation: AAA22798.1.
L23497 Genomic DNA. Translation: AAB38381.1.
PIRiS66094.
RefSeqiNP_387945.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB11840; CAB11840; BSU00640.
GeneIDi938480.
KEGGibsu:BSU00640.
PATRICi18971603. VBIBacSub10457_0065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26185 Genomic DNA. Translation: BAA05299.1 .
U26835 Genomic DNA. Translation: AAB58073.1 .
AL009126 Genomic DNA. Translation: CAB11840.1 .
M29403 Genomic DNA. Translation: AAA22798.1 .
L23497 Genomic DNA. Translation: AAB38381.1 .
PIRi S66094.
RefSeqi NP_387945.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3T91 X-ray 2.64 A/B 590-827 [» ]
3T9Q X-ray 2.76 A/B 590-827 [» ]
ProteinModelPortali P37475.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-126037.
STRINGi 224308.BSU00640.

Protein family/group databases

PptaseDBi P3D0406130.

Proteomic databases

PaxDbi P37475.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB11840 ; CAB11840 ; BSU00640 .
GeneIDi 938480.
KEGGi bsu:BSU00640.
PATRICi 18971603. VBIBacSub10457_0065.

Organism-specific databases

GenoListi BSU00640. [Micado ]

Phylogenomic databases

eggNOGi COG2208.
HOGENOMi HOG000082091.
InParanoidi P37475.
KOi K06382.
OMAi DGMGNGE.
OrthoDBi EOG6JMMQ9.
PhylomeDBi P37475.

Enzyme and pathway databases

BioCyci BSUB:BSU00640-MONOMER.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR014221. Spore_II_E.
[Graphical view ]
Pfami PF07228. SpoIIE. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
TIGRFAMsi TIGR02865. spore_II_E. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
    Ogasawara N., Nakai S., Yoshikawa H.
    DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "Structure and function of the Bacillus SpoIIE protein and its localization to sites of sporulation septum assembly."
    Barak I., Behari J., Olmedo G., Guzman P., Brown D.P., Castro E., Walker D., Westpheling J., Youngman P.
    Mol. Microbiol. 19:1047-1060(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / PY79.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Characterization of the promoter region of the Bacillus subtilis spoIIE operon."
    Guzman P., Westpheling J., Youngman P.
    J. Bacteriol. 170:1598-1609(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
  5. "Characterization of a cell division gene from Bacillus subtilis that is required for vegetative and sporulation septum formation."
    Levin P.A., Losick R.
    J. Bacteriol. 176:1451-1459(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7.
    Strain: 168.
  6. "Activation of cell-specific transcription by a serine phosphatase at the site of asymmetric division."
    Duncan L., Alper S., Arigoni F., Losick R., Stragier P.
    Science 270:641-644(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiSP2E_BACSU
AccessioniPrimary (citable) accession number: P37475
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3