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P37475 (SP2E_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Stage II sporulation protein E
EC=3.1.3.16
Alternative name(s):
Stage II sporulation protein H
Gene names
Name:spoIIE
Synonyms:spoIIH
Ordered Locus Names:BSU00640
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates SpoIIAA by dephosphorylation.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Manganese.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Note: Polar septum.

Sequence similarities

Contains 1 PP2C-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 827827Stage II sporulation protein E
PRO_0000057794

Regions

Transmembrane49 – 6921Helical; Potential
Transmembrane71 – 9121Helical; Potential
Transmembrane116 – 13621Helical; Potential
Transmembrane142 – 16221Helical; Potential
Transmembrane175 – 19521Helical; Potential
Transmembrane206 – 22621Helical; Potential
Transmembrane247 – 26721Helical; Potential
Transmembrane269 – 28921Helical; Potential
Transmembrane299 – 31921Helical; Potential
Transmembrane320 – 34021Helical; Potential
Topological domain341 – 827487Cytoplasmic Potential
Domain605 – 827223PP2C-like
Compositional bias799 – 8024Poly-Val

Secondary structure

1 ...................................... 827
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P37475 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 33EA3A81935B407B

FASTA82791,969
        10         20         30         40         50         60 
MEKAERRVNG PMAGQALEKL QSFFNRGTKL VTHHLHSLFF YKGFIYVVIG FLLGRAFILS 

        70         80         90        100        110        120 
EVLPFALPFF GAMLLIRRDK AFYAVLAVLA GALTISPKHS LLILAALLAF FVFSKVAAFI 

       130        140        150        160        170        180 
TDDRVKALPI VVFFSMAAAR AGFVYAQNGV FTTYDYVMAI VEAGLSFILT LIFLQSLPIF 

       190        200        210        220        230        240 
TVKKVKQSLK IEEIICFMIL IASVLTGLAG LSYQGMQAEH ILARYVVLSF SFIGGASIGC 

       250        260        270        280        290        300 
TVGVVTGLIL GLANIGNLYQ MSLLAFSGLL GGLLKEGKKA GAAIGLIVGS LLISLYGEGS 

       310        320        330        340        350        360 
AGLMTTLYES LIAVCLFLLT PQSITRKVAR YIPGTVEHLQ EQQQYARKIR DVTAQKVDQF 

       370        380        390        400        410        420 
SNVFHALSES FATFYQASDE QTDDSEVDLF LSKITEHSCQ TCYKKNRCWV QNFDKTYDLM 

       430        440        450        460        470        480 
KQVMLETEEK EYASNRRLKK EFQQYCSKSK QVEELIEDEL AHHHAHLTLK KKVQDSRRLV 

       490        500        510        520        530        540 
AEQLLGVSEV MADFSREIKR EREQHFLQEE QIIEALQHFG IEIQHVEIYS LEQGNIDIEM 

       550        560        570        580        590        600 
TIPFSGHGES EKIIAPMLSD ILEEQILVKA EQHSPHPNGY SHVAFGSTKS YRVSTGAAHA 

       610        620        630        640        650        660 
AKGGGLVSGD SYSMMELGAR KYAAAISDGM GNGARAHFES NETIKLLEKI LESGIDEKIA 

       670        680        690        700        710        720 
IKTINSILSL RTTDEIYSTL DLSIIDLQDA SCKFLKVGST PSFIKRGDQV MKVQASNLPI 

       730        740        750        760        770        780 
GIINEFDVEV VSEQLKAGDL LIMMSDGIFE GPKHVENHDL WMKRKMKGLK TNDPQEIADL 

       790        800        810        820 
LMEEVIRTRS GQIEDDMTVV VVRIDHNTPK WASIPVPAIF QNKQEIS

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
Ogasawara N., Nakai S., Yoshikawa H.
DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"Structure and function of the Bacillus SpoIIE protein and its localization to sites of sporulation septum assembly."
Barak I., Behari J., Olmedo G., Guzman P., Brown D.P., Castro E., Walker D., Westpheling J., Youngman P.
Mol. Microbiol. 19:1047-1060(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / PY79.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Characterization of the promoter region of the Bacillus subtilis spoIIE operon."
Guzman P., Westpheling J., Youngman P.
J. Bacteriol. 170:1598-1609(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
[5]"Characterization of a cell division gene from Bacillus subtilis that is required for vegetative and sporulation septum formation."
Levin P.A., Losick R.
J. Bacteriol. 176:1451-1459(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7.
Strain: 168.
[6]"Activation of cell-specific transcription by a serine phosphatase at the site of asymmetric division."
Duncan L., Alper S., Arigoni F., Losick R., Stragier P.
Science 270:641-644(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D26185 Genomic DNA. Translation: BAA05299.1.
U26835 Genomic DNA. Translation: AAB58073.1.
AL009126 Genomic DNA. Translation: CAB11840.1.
M29403 Genomic DNA. Translation: AAA22798.1.
L23497 Genomic DNA. Translation: AAB38381.1.
PIRS66094.
RefSeqNP_387945.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3T91X-ray2.64A/B590-827[»]
3T9QX-ray2.76A/B590-827[»]
ProteinModelPortalP37475.
ModBaseSearch...

Protein-protein interaction databases

STRING224308.BSU00640.

Protein family/group databases

PptaseDBP3D0406130.

Proteomic databases

PaxDbP37475.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB11840; CAB11840; BSU00640.
GeneID938480.
KEGGbsu:BSU00640.
PATRIC18971603. VBIBacSub10457_0065.

Organism-specific databases

GenoListBSU00640. [Micado]

Phylogenomic databases

eggNOGCOG2208.
HOGENOMHOG000082091.
KOK06382.
OMADGMGNGE.
ProtClustDBCLSK872611.

Enzyme and pathway databases

BioCycBSUB:BSU00640-MONOMER.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like.
IPR014221. Spore_II_E.
[Graphical view]
PfamPF07228. SpoIIE. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. PP2C-related. 1 hit.
TIGRFAMsTIGR02865. spore_II_E. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSP2E_BACSU
AccessionPrimary (citable) accession number: P37475
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents