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Protein

Stage II sporulation protein E

Gene

spoIIE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates SpoIIAA by dephosphorylation.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Sporulation

Enzyme and pathway databases

BioCyciBSUB:BSU00640-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Stage II sporulation protein E (EC:3.1.3.16)
Alternative name(s):
Stage II sporulation protein H
Gene namesi
Name:spoIIE
Synonyms:spoIIH
Ordered Locus Names:BSU00640
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Transmembranei142 – 162HelicalSequence analysisAdd BLAST21
Transmembranei175 – 195HelicalSequence analysisAdd BLAST21
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21
Transmembranei247 – 267HelicalSequence analysisAdd BLAST21
Transmembranei269 – 289HelicalSequence analysisAdd BLAST21
Transmembranei299 – 319HelicalSequence analysisAdd BLAST21
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 827CytoplasmicSequence analysisAdd BLAST487

GO - Cellular componenti

  • endospore-forming forespore Source: CACAO
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577941 – 827Stage II sporulation protein EAdd BLAST827

Proteomic databases

PaxDbiP37475.
PRIDEiP37475.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
racAP458703EBI-9304781,EBI-5242400
recAP169712EBI-9304781,EBI-1535844

Protein-protein interaction databases

IntActiP37475. 16 interactors.
MINTiMINT-126037.
STRINGi224308.Bsubs1_010100000325.

Structurei

Secondary structure

1827
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi592 – 600Combined sources9
Beta strandi611 – 617Combined sources7
Turni618 – 620Combined sources3
Beta strandi621 – 627Combined sources7
Beta strandi637 – 640Combined sources4
Helixi642 – 653Combined sources12
Helixi657 – 668Combined sources12
Beta strandi673 – 675Combined sources3
Beta strandi680 – 686Combined sources7
Turni687 – 689Combined sources3
Beta strandi691 – 698Combined sources8
Beta strandi702 – 706Combined sources5
Beta strandi709 – 712Combined sources4
Beta strandi728 – 734Combined sources7
Beta strandi740 – 744Combined sources5
Helixi746 – 749Combined sources4
Beta strandi752 – 754Combined sources3
Helixi758 – 767Combined sources10
Helixi774 – 788Combined sources15
Beta strandi797 – 806Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T91X-ray2.64A/B590-827[»]
3T9QX-ray2.76A/B590-827[»]
ProteinModelPortaliP37475.
SMRiP37475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini594 – 804PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST211

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi799 – 802Poly-Val4

Sequence similaritiesi

Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2208. LUCA.
HOGENOMiHOG000082091.
InParanoidiP37475.
KOiK06382.
OMAiHNTPKWA.
PhylomeDBiP37475.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PPM-type_phosphatase_dom.
IPR014221. Spore_II_E.
[Graphical view]
PfamiPF07228. SpoIIE. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
TIGRFAMsiTIGR02865. spore_II_E. 1 hit.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37475-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKAERRVNG PMAGQALEKL QSFFNRGTKL VTHHLHSLFF YKGFIYVVIG
60 70 80 90 100
FLLGRAFILS EVLPFALPFF GAMLLIRRDK AFYAVLAVLA GALTISPKHS
110 120 130 140 150
LLILAALLAF FVFSKVAAFI TDDRVKALPI VVFFSMAAAR AGFVYAQNGV
160 170 180 190 200
FTTYDYVMAI VEAGLSFILT LIFLQSLPIF TVKKVKQSLK IEEIICFMIL
210 220 230 240 250
IASVLTGLAG LSYQGMQAEH ILARYVVLSF SFIGGASIGC TVGVVTGLIL
260 270 280 290 300
GLANIGNLYQ MSLLAFSGLL GGLLKEGKKA GAAIGLIVGS LLISLYGEGS
310 320 330 340 350
AGLMTTLYES LIAVCLFLLT PQSITRKVAR YIPGTVEHLQ EQQQYARKIR
360 370 380 390 400
DVTAQKVDQF SNVFHALSES FATFYQASDE QTDDSEVDLF LSKITEHSCQ
410 420 430 440 450
TCYKKNRCWV QNFDKTYDLM KQVMLETEEK EYASNRRLKK EFQQYCSKSK
460 470 480 490 500
QVEELIEDEL AHHHAHLTLK KKVQDSRRLV AEQLLGVSEV MADFSREIKR
510 520 530 540 550
EREQHFLQEE QIIEALQHFG IEIQHVEIYS LEQGNIDIEM TIPFSGHGES
560 570 580 590 600
EKIIAPMLSD ILEEQILVKA EQHSPHPNGY SHVAFGSTKS YRVSTGAAHA
610 620 630 640 650
AKGGGLVSGD SYSMMELGAR KYAAAISDGM GNGARAHFES NETIKLLEKI
660 670 680 690 700
LESGIDEKIA IKTINSILSL RTTDEIYSTL DLSIIDLQDA SCKFLKVGST
710 720 730 740 750
PSFIKRGDQV MKVQASNLPI GIINEFDVEV VSEQLKAGDL LIMMSDGIFE
760 770 780 790 800
GPKHVENHDL WMKRKMKGLK TNDPQEIADL LMEEVIRTRS GQIEDDMTVV
810 820
VVRIDHNTPK WASIPVPAIF QNKQEIS
Length:827
Mass (Da):91,969
Last modified:October 1, 1994 - v1
Checksum:i33EA3A81935B407B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05299.1.
U26835 Genomic DNA. Translation: AAB58073.1.
AL009126 Genomic DNA. Translation: CAB11840.1.
M29403 Genomic DNA. Translation: AAA22798.1.
L23497 Genomic DNA. Translation: AAB38381.1.
PIRiS66094.
RefSeqiNP_387945.1. NC_000964.3.
WP_003243026.1. NZ_JNCM01000028.1.

Genome annotation databases

EnsemblBacteriaiCAB11840; CAB11840; BSU00640.
GeneIDi938480.
KEGGibsu:BSU00640.
PATRICi18971603. VBIBacSub10457_0065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05299.1.
U26835 Genomic DNA. Translation: AAB58073.1.
AL009126 Genomic DNA. Translation: CAB11840.1.
M29403 Genomic DNA. Translation: AAA22798.1.
L23497 Genomic DNA. Translation: AAB38381.1.
PIRiS66094.
RefSeqiNP_387945.1. NC_000964.3.
WP_003243026.1. NZ_JNCM01000028.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T91X-ray2.64A/B590-827[»]
3T9QX-ray2.76A/B590-827[»]
ProteinModelPortaliP37475.
SMRiP37475.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP37475. 16 interactors.
MINTiMINT-126037.
STRINGi224308.Bsubs1_010100000325.

Proteomic databases

PaxDbiP37475.
PRIDEiP37475.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11840; CAB11840; BSU00640.
GeneIDi938480.
KEGGibsu:BSU00640.
PATRICi18971603. VBIBacSub10457_0065.

Phylogenomic databases

eggNOGiCOG2208. LUCA.
HOGENOMiHOG000082091.
InParanoidiP37475.
KOiK06382.
OMAiHNTPKWA.
PhylomeDBiP37475.

Enzyme and pathway databases

BioCyciBSUB:BSU00640-MONOMER.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PPM-type_phosphatase_dom.
IPR014221. Spore_II_E.
[Graphical view]
PfamiPF07228. SpoIIE. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
TIGRFAMsiTIGR02865. spore_II_E. 1 hit.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSP2E_BACSU
AccessioniPrimary (citable) accession number: P37475
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.