Reviewed, UniProtKB/Swiss-Prot P37475 (SP2E_BACSU)

Names and origin

Protein names

Recommended name:
    Stage II sporulation protein E
    EC=3.1.3.16
Alternative name(s):
    Stage II sporulation protein H

Gene names

Name:	spoIIE
Synonyms:	spoIIH
Ordered Locus Names:	BSU00640

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates spoIIAA by dephosphorylation.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Manganese.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Note: Polar septum.
Sequence similarities	Contains 1 PP2C-like domain.

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 827	827	Stage II sporulation protein E		PRO_0000057794
Regions
	Transmembrane	49 – 69	21	Potential
	Transmembrane	71 – 91	21	Potential
	Transmembrane	116 – 136	21	Potential
	Transmembrane	142 – 162	21	Potential
	Transmembrane	175 – 195	21	Potential
	Transmembrane	206 – 226	21	Potential
	Transmembrane	247 – 267	21	Potential
	Transmembrane	269 – 289	21	Potential
	Transmembrane	299 – 319	21	Potential
	Transmembrane	320 – 340	21	Potential
	Topological domain	341 – 827	487	Cytoplasmic Potential
	Domain	605 – 827	223	PP2C-like
	Compositional bias	799 – 802	4	Poly-Val

Sequences

	Sequence		Length	Mass (Da)
	P37475-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 33EA3A81935B407B	FASTA	827	91,969
	10 20 30 40 50 60 MEKAERRVNG PMAGQALEKL QSFFNRGTKL VTHHLHSLFF YKGFIYVVIG FLLGRAFILS 70 80 90 100 110 120 EVLPFALPFF GAMLLIRRDK AFYAVLAVLA GALTISPKHS LLILAALLAF FVFSKVAAFI 130 140 150 160 170 180 TDDRVKALPI VVFFSMAAAR AGFVYAQNGV FTTYDYVMAI VEAGLSFILT LIFLQSLPIF 190 200 210 220 230 240 TVKKVKQSLK IEEIICFMIL IASVLTGLAG LSYQGMQAEH ILARYVVLSF SFIGGASIGC 250 260 270 280 290 300 TVGVVTGLIL GLANIGNLYQ MSLLAFSGLL GGLLKEGKKA GAAIGLIVGS LLISLYGEGS 310 320 330 340 350 360 AGLMTTLYES LIAVCLFLLT PQSITRKVAR YIPGTVEHLQ EQQQYARKIR DVTAQKVDQF 370 380 390 400 410 420 SNVFHALSES FATFYQASDE QTDDSEVDLF LSKITEHSCQ TCYKKNRCWV QNFDKTYDLM 430 440 450 460 470 480 KQVMLETEEK EYASNRRLKK EFQQYCSKSK QVEELIEDEL AHHHAHLTLK KKVQDSRRLV 490 500 510 520 530 540 AEQLLGVSEV MADFSREIKR EREQHFLQEE QIIEALQHFG IEIQHVEIYS LEQGNIDIEM 550 560 570 580 590 600 TIPFSGHGES EKIIAPMLSD ILEEQILVKA EQHSPHPNGY SHVAFGSTKS YRVSTGAAHA 610 620 630 640 650 660 AKGGGLVSGD SYSMMELGAR KYAAAISDGM GNGARAHFES NETIKLLEKI LESGIDEKIA 670 680 690 700 710 720 IKTINSILSL RTTDEIYSTL DLSIIDLQDA SCKFLKVGST PSFIKRGDQV MKVQASNLPI 730 740 750 760 770 780 GIINEFDVEV VSEQLKAGDL LIMMSDGIFE GPKHVENHDL WMKRKMKGLK TNDPQEIADL 790 800 810 820 LMEEVIRTRS GQIEDDMTVV VVRIDHNTPK WASIPVPAIF QNKQEIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"Structure and function of the Bacillus SpoIIE protein and its localization to sites of sporulation septum assembly." Barak I., Behari J., Olmedo G., Guzman P., Brown D.P., Castro E., Walker D., Westpheling J., Youngman P. Mol. Microbiol. 19:1047-1060(1996) [PubMed: 8830262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / PY79.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Characterization of the promoter region of the Bacillus subtilis spoIIE operon." Guzman P., Westpheling J., Youngman P. J. Bacteriol. 170:1598-1609(1988) [PubMed: 2832371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
[5]	"Characterization of a cell division gene from Bacillus subtilis that is required for vegetative and sporulation septum formation." Levin P.A., Losick R. J. Bacteriol. 176:1451-1459(1994) [PubMed: 8113187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7. Strain: 168.
[6]	"Activation of cell-specific transcription by a serine phosphatase at the site of asymmetric division." Duncan L., Alper S., Arigoni F., Losick R., Stragier P. Science 270:641-644(1995) [PubMed: 7570023] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D26185 Genomic DNA. Translation: BAA05299.1. U26835 Genomic DNA. Translation: AAB58073.1. AL009126 Genomic DNA. Translation: CAB11840.1. M29403 Genomic DNA. Translation: AAA22798.1. L23497 Genomic DNA. Translation: AAB38381.1.
PIR	S66094.
RefSeq	NP_387945.1.
3D structure databases
ModBase	Search...
Protein family/group databases
PptaseDB	P3D0406130.
Genome annotation databases
GeneID	938480.
GenomeReviews	Gene locus BSU00640 in contig AL009126_GR.
KEGG	bsu:BSU00640.
NMPDR	fig|224308.1.peg.64.
Organism-specific databases
SubtiList	BG10127. spoIIE. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG314042.
OMA	ISDGMGN.
Enzyme and pathway databases
BRENDA	3.1.3.16. 150.
Family and domain databases
InterPro	IPR001932. PP2C-related. IPR014221. Spore_II_E. IPR010822. Spore_II_E_C. [Graphical view]
Gene3D	G3DSA:3.60.40.10. PP2C-related. 1 hit.
Pfam	PF07228. SpoIIE. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SP2E_BACSU

Accession

Primary (citable) accession number: P37475

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 15, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)