Reviewed, UniProtKB/Swiss-Prot P37474 (MFD_BACSU)

Names and origin

Protein names

Recommended name:
    Transcription-repair-coupling factor
      Short name=TRCF
    EC=3.6.1.-
Alternative name(s):
    ATP-dependent helicase mfd

Gene names

Name:	mfd
Ordered Locus Names:	BSU00550

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	1177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Necessary for strand-specific repair. A lesion in the template strand blocks the RNA polymerase complex (RNAP). The RNAP-DNA-RNA complex is specifically recognized by TRCF which releases RNAP and the truncated transcript; the TCRF may replace RNAP at the lesion site and then recruit the uvrA/B/C repair system By similarity.
Sequence similarities	In the N-terminal section; belongs to the uvrB family. In the C-terminal section; belongs to the helicase family. RecG subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro damaged DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1177	1177	Transcription-repair-coupling factor		PRO_0000102162
Regions
	Domain	638 – 799	162	Helicase ATP-binding
	Domain	820 – 974	155	Helicase C-terminal
	Nucleotide binding	651 – 658	8	ATP Potential
	Motif	752 – 755	4	DEEQ box

Sequences

	Sequence		Length	Mass (Da)
	P37474-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 70938BC354424687	FASTA	1,177	133,812
	10 20 30 40 50 60 MDNIQTFIKE SDDFKSIING LHEGLKEQLL AGLSGSARSV FTSALANETN KPIFLITHNL 70 80 90 100 110 120 YQAQKVTDDL TSLLEDRSVL LYPVNELISS EIAVASPELR AQRLDVINRL TNGEAPIVVA 130 140 150 160 170 180 PVAAIRRMLP PVEVWKSSQM LIQVGHDIEP DQLASRLVEV GYERSDMVSA PGEFSIRGGI 190 200 210 220 230 240 IDIYPLTSEN PVRIELFDTE VDSIRSFNSD DQRSIETLTS INIGPAKELI IRPEEKARAM 250 260 270 280 290 300 EKIDSGLAAS LKKLKADKQK EILHANISHD KERLSEGQTD QELVKYLSYF YEKPASLLDY 310 320 330 340 350 360 TPDNTLLILD EVSRIHEMEE QLQKEEAEFI TNLLEEGKIL HDIRLSFSFQ KIVAEQKRPL 370 380 390 400 410 420 LYYSLFLRHV HHTSPQNIVN VSGRQMQSFH GQMNVLAGEM ERFKKSNFTV VFLGANKERT 430 440 450 460 470 480 QKLSSVLADY DIEAAMTDSK KALVQGQVYI MEGELQSGFE LPLMKLAVIT EEELFKNRVK 490 500 510 520 530 540 KKPRKQKLTN AERIKSYSEL QIGDYVVHIN HGIGKYLGIE TLEINGIHKD YLNIHYQGSD 550 560 570 580 590 600 KLYVPVEQID QVQKYVGSEG KEPKLYKLGG SEWKRVKKKV ETSVQDIADD LIKLYAEREA 610 620 630 640 650 660 SKGYAFSPDH EMQREFESAF PYQETEDQLR SIHEIKKDME RERPMDRLLC GDVGYGKTEV 670 680 690 700 710 720 AIRAAFKAIG DGKQVALLVP TTILAQQHYE TIKERFQDYP INIGLLSRFR TRKEANETIK 730 740 750 760 770 780 GLKNGTVDIV IGTHRLLSKD VVYKDLGLLI IDEEQRFGVT HKEKIKQIKA NVDVLTLTAT 790 800 810 820 830 840 PIPRTLHMSM LGVRDLSVIE TPPENRFPVQ TYVVEYNGAL VREAIERELA RGGQVYFLYN 850 860 870 880 890 900 RVEDIERKAD EISMLVPDAK VAYAHGKMTE NELETVMLSF LEGESDVLVS TTIIETGVDI 910 920 930 940 950 960 PNVNTLIVFD ADKMGLSQLY QLRGRVGRSN RVAYAYFTYR RDKVLTEVAE KRLQAIKEFT 970 980 990 1000 1010 1020 ELGSGFKIAM RDLTIRGAGN LLGAQQHGFI DSVGFDLYSQ MLKEAIEERK GDTAKTEQFE 1030 1040 1050 1060 1070 1080 TEIDVELDAY IPETYIQDGK QKIDMYKRFR SVATIEEKNE LQDEMIDRFG NYPKEVEYLF 1090 1100 1110 1120 1130 1140 TVAEMKVYAR QERVELIKQD KDAVRLTISE EASAEIDGQK LFELGNQYGR QIGLGMEGKK 1150 1160 1170 LKISIQTKGR SADEWLDTVL GMLKGLKDVK KQTISST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D26185 Genomic DNA. Translation: BAA05290.1. AL009126 Genomic DNA. Translation: CAB11831.1.
PIR	S66085.
RefSeq	NP_387936.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	936653.
GenomeReviews	Gene locus BSU00550 in contig AL009126_GR.
KEGG	bsu:BSU00550.
NMPDR	fig|224308.1.peg.55.
Organism-specific databases
SubtiList	BG10118. mfd. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG616165.
OMA	IQTYVME.
Family and domain databases
InterPro	IPR003711. CarD_TRCF. IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR004576. Mfd. IPR005118. Transcpt_repair-coupling_fac. [Graphical view]
Pfam	PF02559. CarD_TRCF. 1 hit. PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. PF03461. TRCF. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MFD_BACSU

Accession

Primary (citable) accession number: P37474

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 15, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)