Reviewed, UniProtKB/Swiss-Prot P37473 (ADH_DROPE)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase
    EC=1.1.1.1

Gene names

Name:	Adh
ORF Names:	GL25993

Organism

Drosophila persimilis (Fruit fly)

Taxonomic identifier

7234 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Sequence caution	The sequence AAB80702.1 differs from that shown. Reason: Frameshift at position 186. The sequence AAB80704.1 differs from that shown. Reason: Frameshift at position 186. The sequence AAB80708.1 differs from that shown. Reason: Frameshift at position 186. The sequence AAB80710.1 differs from that shown. Reason: Frameshift at position 186.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alcohol dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 254	253	Alcohol dehydrogenase		PRO_0000054488
Regions
	Nucleotide binding	10 – 33	24	NAD By similarity
Sites
	Active site	151	1	Proton acceptor By similarity
	Binding site	138	1	Substrate By similarity
Natural variations
	Natural variant	82	1	V → L in strain: Persimi50. Ref.2
	Natural variant	185	1	R → K in strain: Persimi44. Ref.2
Experimental info
	Sequence conflict	16	1	G → A in AAB80702. Ref.2
	Sequence conflict	16	1	G → A in AAB80704. Ref.2
	Sequence conflict	16	1	G → A in AAB80706. Ref.2
	Sequence conflict	16	1	G → A in AAB80708. Ref.2
	Sequence conflict	16	1	G → A in AAB80710. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P37473-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C76A72252371F0B6	FASTA	254	27,645
	10 20 30 40 50 60 MSLTNKNVVF VAGLGGIGLD TSRELVKRNL KNLVILDRID NPAAIAELKA INPKVTITFY 70 80 90 100 110 120 PYDVTVPVAE TTKLLKTIFA QVKTIDVLIN GAGILDDHQI ERTIAVNYTG LVNTTTAILD 130 140 150 160 170 180 FWDKRKGGPG GIICNIGSVT GFNAIYQVPV YSGSKAAVVN FTSSLAKLAP ITGVTAYTVN 190 200 210 220 230 240 PGITRTTLVH KFNSWLDVEP RVAEKLLEHP TQTSQQCAEN FVKAIELNKN GAIWKLDLGT 250 LEPITWTQHW DSGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis of Adh genes estimates the time of geographic isolation of the Bogota population of Drosophila pseudoobscura." Schaeffer S.W., Miller E.L. Proc. Natl. Acad. Sci. U.S.A. 88:6097-6101(1991) [PubMed: 2068088] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Gene flow and natural selection in the origin of Drosophila pseudoobscura and close relatives." Wang R.L., Wakeley J., Hey J. Genetics 147:1091-1106(1997) [PubMed: 9383055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-82 AND LYS-185. Strain: Persimi40, Persimi42, Persimi44, Persimi49 and Persimi50.
[3]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 14011-0111.49.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60997 Genomic DNA. Translation: AAA99035.1. AF006564 Genomic DNA. Translation: AAB80702.1. Frameshift. AF006565 Genomic DNA. Translation: AAB80704.1. Frameshift. AF006566 Genomic DNA. Translation: AAB80706.1. AF006567 Genomic DNA. Translation: AAB80708.1. Frameshift. AF006568 Genomic DNA. Translation: AAB80710.1. Frameshift. CH479184 Genomic DNA. Translation: EDW36993.1.
PIR	S26784.
RefSeq	XP_002018797.1.
3D structure databases
HSSP	HSSP built from PDB template 1B16 based on UniProtKB P10807.
SMR	P37473. Positions 2-254.
ModBase	Search...
Genome annotation databases
GeneID	6593284.
KEGG	dpe:Dper_GL25993.
Organism-specific databases
FlyBase	FBgn0012635. Dper\Adh.
Enzyme and pathway databases
BRENDA	1.1.1.1. 190796.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002425. Insect_ADH. IPR002424. Insect_AlcDH_fam. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR01168. ALCDHDRGNASE. PR01167. INSADHFAMILY. PR00080. SDRFAMILY.
ProDom	PD000819. SRP54. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADH_DROPE

Accession

Primary (citable) accession number: P37473
Secondary accession number(s): B4GK27 , O16093, O16095, O16097, O18661, Q7KQD6, Q7KQD7, Q7KQD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project