P37468 (RSMA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase A
EC=2.1.1.182

Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Gene names

Name:	rsmA
Synonyms:	ksgA
Ordered Locus Names:	BSU00420

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP MF_00607
Catalytic activity	4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP MF_00607
Subcellular location	Cytoplasm Potential HAMAP MF_00607.
Sequence similarities	Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Ribosomal RNA small subunit methyltransferase A HAMAP MF_00607		PRO_0000101485
Sites
	Binding site	29	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	31	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	56	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	77	1	S-adenosyl-L-methionine By similarity
	Binding site	102	1	S-adenosyl-L-methionine By similarity
	Binding site	127	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37468 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 26CE063F33BA8994	FASTA	292	32,721
	10 20 30 40 50 60 MNKDIATPIR TKEILKKYGF SFKKSLGQNF LIDTNILNRI VDHAEVTEKT GVIEIGPGIG 70 80 90 100 110 120 ALTEQLAKRA KKVVAFEIDQ RLLPILKDTL SPYENVTVIH QDVLKADVKS VIEEQFQDCD 130 140 150 160 170 180 EIMVVANLPY YVTTPIIMKL LEEHLPLKGI VVMLQKEVAE RMAADPSSKE YGSLSIAVQF 190 200 210 220 230 240 YTEAKTVMIV PKTVFVPQPN VDSAVIRLIL RDGPAVDVEN ESFFFQLIKA SFAQRRKTLL 250 260 270 280 290 NNLVNNLPEG KAQKSTIEQV LEETNIDGKR RGESLSIEEF AALSNGLYKA LF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26185 Genomic DNA. Translation: BAA05277.1. AL009126 Genomic DNA. Translation: CAB11818.1.
PIR	S66071.
RefSeq	NP_387923.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P37468.
SMR	P37468. Positions 27-291.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000003412; EBBACP00000003412; EBBACG00000003405.
GeneID	936358.
GenomeReviews	Gene locus BSU00420 in contig AL009126_GR.
KEGG	bsu:BSU00420.
NMPDR	fig|224308.1.peg.42.
PATRIC	18971555. VBIBacSub10457_0043.
Organism-specific databases
GenoList	BSU00420. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000002781.
HOGENOM	HBG319664.
OMA	LPHPAKD.
PhylomeDB	P37468.
ProtClustDB	PRK00274.
Enzyme and pathway databases
BioCyc	BSUB:BSU00420-MONOMER.
Family and domain databases
HAMAP	MF_00607. 16SrRNA_methyltr_A. [Tree]
InterPro	IPR023165. rRNA_Ade_diMease-like. IPR020596. rRNA_Ade_Mease_Trfase_CS. IPR001737. rRNA_Ade_methylase_transferase. IPR020598. rRNA_Ade_methylase_Trfase_N. IPR011530. rRNA_adenine_dimethylase. [Graphical view]
Gene3D	G3DSA:1.10.8.100. rRNA_Ade_diMease-like. 1 hit.
KO	K02528.
PANTHER	PTHR11727. RRNA_meth_trans. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00755. KsgA. 1 hit.
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMA_BACSU

Accession

Primary (citable) accession number: P37468

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program