Reviewed, UniProtKB/Swiss-Prot P37468 (KSGA_BACSU)

Names and origin

Protein names

Recommended name:
    Dimethyladenosine transferase
    EC=2.1.1.-
Alternative name(s):
    S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
    16S rRNA dimethylase
    High level kasugamycin resistance protein ksgA
    Kasugamycin dimethyltransferase

Gene names

Name:	ksgA
Ordered Locus Names:	BSU00420

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. Its inactivation leads to kasugamycin resistance By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance rRNA processing
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: HAMAP response to antibiotic Inferred from electronic annotation. Source: HAMAP
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Dimethyladenosine transferase HAMAP MF_00607		PRO_0000101485
Sites
	Binding site	29	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	31	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	56	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	77	1	S-adenosyl-L-methionine By similarity
	Binding site	102	1	S-adenosyl-L-methionine By similarity
	Binding site	127	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37468-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 26CE063F33BA8994	FASTA	292	32,721
	10 20 30 40 50 60 MNKDIATPIR TKEILKKYGF SFKKSLGQNF LIDTNILNRI VDHAEVTEKT GVIEIGPGIG 70 80 90 100 110 120 ALTEQLAKRA KKVVAFEIDQ RLLPILKDTL SPYENVTVIH QDVLKADVKS VIEEQFQDCD 130 140 150 160 170 180 EIMVVANLPY YVTTPIIMKL LEEHLPLKGI VVMLQKEVAE RMAADPSSKE YGSLSIAVQF 190 200 210 220 230 240 YTEAKTVMIV PKTVFVPQPN VDSAVIRLIL RDGPAVDVEN ESFFFQLIKA SFAQRRKTLL 250 260 270 280 290 NNLVNNLPEG KAQKSTIEQV LEETNIDGKR RGESLSIEEF AALSNGLYKA LF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D26185 Genomic DNA. Translation: BAA05277.1. AL009126 Genomic DNA. Translation: CAB11818.1.
PIR	S66071.
RefSeq	NP_387923.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	936358.
GenomeReviews	Gene locus BSU00420 in contig AL009126_GR.
KEGG	bsu:BSU00420.
NMPDR	fig|224308.1.peg.42.
Organism-specific databases
SubtiList	BG10105. ksgA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P37468.
OMA	LGNIPYS
Family and domain databases
HAMAP	MF_00607. [Tree]
InterPro	IPR020596. rRNA_Ade_Mease_Trfase_CS. IPR001737. rRNA_Ade_methylase_transferase. IPR020598. rRNA_Ade_methylase_Trfase_N. IPR011530. rRNA_adenine_dimethylase. [Graphical view]
PANTHER	PTHR11727. RRNA_meth_trans. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00755. ksgA. 1 hit.
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KSGA_BACSU

Accession

Primary (citable) accession number: P37468

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 24, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)