Reviewed, UniProtKB/Swiss-Prot P37465 (SYM_BACSU)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Synonyms:	metS
Ordered Locus Names:	BSU00380

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	664 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_01228
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228
Subunit structure	Homodimer By similarity. HAMAP MF_01228
Subcellular location	Cytoplasm HAMAP MF_01228.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 664	664	Methionyl-tRNA synthetase HAMAP MF_01228		PRO_0000139211
Regions
	Domain	570 – 662	93	tRNA-binding
	Motif	15 – 25	11	"HIGH" region HAMAP MF_01228
	Motif	311 – 315	5	"KMSKS" region HAMAP MF_01228
Sites
	Binding site	314	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37465-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: FF4C0575169E82DE	FASTA	664	76,188
	10 20 30 40 50 60 MPQENNTFYI TTPIYYPSGK LHIGHAYTTV AGDAMARYKR LKGFDVRYLT GTDEHGQKIQ 70 80 90 100 110 120 QKAEQENITP QEYVDRAAAD IQKLWKQLEI SNDDFIRTTE KRHKVVIEKV FQKLLDNGDI 130 140 150 160 170 180 YLDEYEGWYS IPDETFYTET QLVDIERNEK GEVIGGKSPD SGHPVELIKE ESYFFRMGKY 190 200 210 220 230 240 ADRLLKYYEE NPTFIQPESR KNEMINNFIK PGLEDLAVSR TTFDWGVKVP ENPKHVVYVW 250 260 270 280 290 300 IDALFNYLTA LGYDTENDEL YQKYWPADVH LVGKEIVRFH TIYWPIMLMA LDLPLPKQVF 310 320 330 340 350 360 AHGWLLMKDG KMSKSKGNVV DPVTLIERYG LDELRYYLLR EVPFGSDGVF TPEGFVERIN 370 380 390 400 410 420 YDLANDLGNL LNRTVAMINK YFDGQIGSYK GAVTEFDHTL TSVAEETVKA YEKAMENMEF 430 440 450 460 470 480 SVALSTLWQL ISRTNKYIDE TAPWVLAKDP AKEEELRSVM YHLAESLRIS AVLLQPFLTK 490 500 510 520 530 540 TPEKMFEQLG ITDESLKAWD SITAFGQLKD TKVQKGEPLF PRLEAEEEIA YIKGKMQGSA 550 560 570 580 590 600 PAKEETKEEE PQEVDRLPEI TIDQFMDVEL RVAEVIEAEP VKKADRLLKL QLDLGFEKRQ 610 620 630 640 650 660 VVSGIAKHYT PEELVGKKLV CVTNLKPVKL RGELSQGMIL AGEADGVLKV VSIDQSLPKG TRIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26185 Genomic DNA. Translation: BAA05273.1. AL009126 Genomic DNA. Translation: CAB11814.1.
PIR	S66067.
RefSeq	NP_387919.1.
3D structure databases
SMR	P37465. Positions 436-662, 558-663.
ModBase	Search...
Genome annotation databases
GeneID	936877.
GenomeReviews	Gene locus BSU00380 in contig AL009126_GR.
KEGG	bsu:BSU00380.
NMPDR	fig|224308.1.peg.38.
Organism-specific databases
SubtiList	BG10101. metG. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG721667.
OMA	DWGIKVP.
PhylomeDB	P37465.
Enzyme and pathway databases
BRENDA	6.1.1.10. 150.
Family and domain databases
HAMAP	MF_01228. Met_tRNA_synth_type2. Fused. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BACSU

Accession

Primary (citable) accession number: P37465

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	February 9, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)