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Protein

Single-stranded DNA-binding protein A

Gene

ssbA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism (PubMed:21170359). Has a 20-fold higher affinity for ssDNA than SsbB; SsbA and DprA activate the homologuos DNA strand exchange function of RecA-ATP (PubMed:25138221).1 Publication1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU40900-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Single-stranded DNA-binding protein AUniRule annotation
Short name:
SSB AUniRule annotation
Gene namesi
Name:ssbA
Ordered Locus Names:BSU40900
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172Single-stranded DNA-binding protein APRO_0000096005Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei82 – 821Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylated by YwqD, which increases ssDNA affinity; dephosphorylated by YwqE.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP37455.

PTM databases

iPTMnetiP37455.

Expressioni

Inductioni

Strongly expressed during exponential growth, decreases 2-4-fold in stationary phase, part of the rpsF-ssbA-rpsR and the ychF-rpsF-ssbA-rpsR operons (PubMed:14762004). The operon is induced by DNA damage by mitomycin C (PubMed:14762004).1 Publication

Interactioni

Subunit structurei

Homotetramer. Interacts with several proteins involved in DNA metabolism such as PriA, RecQ, RecG, RecS, DnaE, RarA, RecJ, RecO, SbcC, YrrC, XseA and Ung.UniRule annotation2 Publications

Protein-protein interaction databases

IntActiP37455. 1 interaction.
STRINGi224308.Bsubs1_010100022076.

Structurei

3D structure databases

ProteinModelPortaliP37455.
SMRiP37455. Positions 2-103.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 104104SSBUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi167 – 1726Important for interaction with partner proteinsUniRule annotation

Sequence similaritiesi

Contains 1 SSB domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UUM. Bacteria.
COG0629. LUCA.
HOGENOMiHOG000106484.
InParanoidiP37455.
KOiK03111.
OMAiEETEWHD.
PhylomeDBiP37455.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
HAMAPiMF_00984. SSB. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR000424. Primosome_PriB/ssb.
IPR011344. ssDNA-bd.
[Graphical view]
PANTHERiPTHR10302. PTHR10302. 1 hit.
PfamiPF00436. SSB. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00621. ssb. 1 hit.
PROSITEiPS50935. SSB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37455-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNRVVLVGR LTKDPELRYT PNGAAVATFT LAVNRTFTNQ SGEREADFIN
60 70 80 90 100
CVTWRRQAEN VANFLKKGSL AGVDGRLQTR NYENQQGQRV FVTEVQAESV
110 120 130 140 150
QFLEPKNGGG SGSGGYNEGN SGGGQYFGGG QNDNPFGGNQ NNQRRNQGNS
160 170
FNDDPFANDG KPIDISDDDL PF
Length:172
Mass (Da):18,742
Last modified:October 1, 1994 - v1
Checksum:iC170025FBDACDC5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05220.1.
AL009126 Genomic DNA. Translation: CAB16127.1.
PIRiS66014.
RefSeqiNP_391970.1. NC_000964.3.
WP_003219228.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB16127; CAB16127; BSU40900.
GeneIDi937911.
KEGGibsu:BSU40900.
PATRICi18980278. VBIBacSub10457_4297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05220.1.
AL009126 Genomic DNA. Translation: CAB16127.1.
PIRiS66014.
RefSeqiNP_391970.1. NC_000964.3.
WP_003219228.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliP37455.
SMRiP37455. Positions 2-103.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP37455. 1 interaction.
STRINGi224308.Bsubs1_010100022076.

PTM databases

iPTMnetiP37455.

Proteomic databases

PaxDbiP37455.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB16127; CAB16127; BSU40900.
GeneIDi937911.
KEGGibsu:BSU40900.
PATRICi18980278. VBIBacSub10457_4297.

Phylogenomic databases

eggNOGiENOG4108UUM. Bacteria.
COG0629. LUCA.
HOGENOMiHOG000106484.
InParanoidiP37455.
KOiK03111.
OMAiEETEWHD.
PhylomeDBiP37455.

Enzyme and pathway databases

BioCyciBSUB:BSU40900-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
HAMAPiMF_00984. SSB. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR000424. Primosome_PriB/ssb.
IPR011344. ssDNA-bd.
[Graphical view]
PANTHERiPTHR10302. PTHR10302. 1 hit.
PfamiPF00436. SSB. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00621. ssb. 1 hit.
PROSITEiPS50935. SSB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSBA_BACSU
AccessioniPrimary (citable) accession number: P37455
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.