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Protein

Exodeoxyribonuclease

Gene

exoA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Probably binds two magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Magnesium 1By similarity1
Active sitei106By similarity1
Active sitei145Proton donor/acceptorBy similarity1
Metal bindingi145Magnesium 2By similarity1
Metal bindingi147Magnesium 2By similarity1
Sitei147Transition state stabilizerBy similarity1
Sitei217Important for catalytic activityBy similarity1
Metal bindingi242Magnesium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU40880-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exodeoxyribonuclease (EC:3.1.11.2)
Gene namesi
Name:exoA
Ordered Locus Names:BSU40880
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002000251 – 252ExodeoxyribonucleaseAdd BLAST252

Proteomic databases

PaxDbiP37454.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei243Interaction with DNA substrateBy similarity1

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100022066.

Structurei

Secondary structure

1252
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi11 – 17Combined sources7
Helixi20 – 27Combined sources8
Beta strandi30 – 35Combined sources6
Beta strandi41 – 44Combined sources4
Beta strandi52 – 56Combined sources5
Beta strandi58 – 60Combined sources3
Beta strandi66 – 72Combined sources7
Beta strandi75 – 80Combined sources6
Helixi84 – 86Combined sources3
Beta strandi87 – 89Combined sources3
Beta strandi92 – 96Combined sources5
Beta strandi101 – 106Combined sources6
Helixi112 – 114Combined sources3
Helixi117 – 137Combined sources21
Beta strandi140 – 145Combined sources6
Helixi152 – 154Combined sources3
Helixi158 – 160Combined sources3
Helixi169 – 180Combined sources12
Beta strandi183 – 185Combined sources3
Helixi186 – 190Combined sources5
Beta strandi199 – 201Combined sources3
Turni205 – 211Combined sources7
Beta strandi217 – 222Combined sources6
Helixi223 – 228Combined sources6
Beta strandi229 – 234Combined sources6
Beta strandi240 – 243Combined sources4
Beta strandi246 – 250Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CFEX-ray1.84A1-252[»]
ProteinModelPortaliP37454.
SMRiP37454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA repair enzymes AP/ExoA family.Curated

Phylogenomic databases

eggNOGiENOG4105CGK. Bacteria.
COG0708. LUCA.
HOGENOMiHOG000034586.
InParanoidiP37454.
KOiK01142.
OMAiYTPNSQQ.
PhylomeDBiP37454.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR22748. PTHR22748. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
TIGRFAMsiTIGR00633. xth. 1 hit.
PROSITEiPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLISWNVNG LRAVMRKMDF LSYLKEEDAD IICLQETKIQ DGQVDLQPED
60 70 80 90 100
YHVYWNYAVK KGYSGTAVFS KQEPLQVIYG IGVEEHDQEG RVITLEFENV
110 120 130 140 150
FVMTVYTPNS RRGLERIDYR MQWEEALLSY ILELDQKKPV ILCGDLNVAH
160 170 180 190 200
QEIDLKNPKA NRNNAGFSDQ EREAFTRFLE AGFVDSFRHV YPDLEGAYSW
210 220 230 240 250
WSYRAGARDR NIGWRIDYFV VSESLKEQIE DASISADVMG SDHCPVELII

NI
Length:252
Mass (Da):29,252
Last modified:October 1, 1994 - v1
Checksum:i08F3636B70FE6625
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05218.1.
AL009126 Genomic DNA. Translation: CAB16125.1.
PIRiS66012.
RefSeqiNP_391968.1. NC_000964.3.
WP_003243194.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB16125; CAB16125; BSU40880.
GeneIDi937918.
KEGGibsu:BSU40880.
PATRICi18980274. VBIBacSub10457_4295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05218.1.
AL009126 Genomic DNA. Translation: CAB16125.1.
PIRiS66012.
RefSeqiNP_391968.1. NC_000964.3.
WP_003243194.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CFEX-ray1.84A1-252[»]
ProteinModelPortaliP37454.
SMRiP37454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100022066.

Proteomic databases

PaxDbiP37454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB16125; CAB16125; BSU40880.
GeneIDi937918.
KEGGibsu:BSU40880.
PATRICi18980274. VBIBacSub10457_4295.

Phylogenomic databases

eggNOGiENOG4105CGK. Bacteria.
COG0708. LUCA.
HOGENOMiHOG000034586.
InParanoidiP37454.
KOiK01142.
OMAiYTPNSQQ.
PhylomeDBiP37454.

Enzyme and pathway databases

BioCyciBSUB:BSU40880-MONOMER.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR22748. PTHR22748. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
TIGRFAMsiTIGR00633. xth. 1 hit.
PROSITEiPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEXOA_BACSU
AccessioniPrimary (citable) accession number: P37454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.