Reviewed, UniProtKB/Swiss-Prot P37447 (PA1_PROVU)

Names and origin

Protein names

Recommended name:
    Phospholipase A1
    EC=3.1.1.32
    EC=3.1.1.4
Alternative name(s):
    Detergent-resistant phospholipase A
      Short name=DR-phospholipase A
    Phosphatidylcholine 1-acylhydrolase
    Outer membrane phospholipase A
      Short name=OM PLA

Gene names

Name:

pldA

Organism

Proteus vulgaris

Taxonomic identifier

585 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities.
Catalytic activity	Phosphatidylcholine + H(2)O = 2-acylglycerophosphocholine + a carboxylate. Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Calcium.
Subcellular location	Cell outer membrane. Note= One of the very few enzymes located there.
Sequence similarities	Belongs to the phospholipase A1 family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
Ligand	Calcium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A1 activity Inferred from electronic annotation. Source: EC phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 289	269	Phospholipase A1		PRO_0000021987
Sites
	Active site	164	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37447-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: D75516CFFB406997	FASTA	289	32,945
	10 20 30 40 50 60 MRTGPGWLLA AAALPFFACA QEATIDKVHD TPAVRGSIIA NMLQEHDNPF TLYPYESNYL 70 80 90 100 110 120 LYTYTSDLNK KAIESYNWSD NANKDEVKFQ LSLAFPLWRG ILGDNSLLGA SYTQRSWWQL 130 140 150 160 170 180 SNTGESAPFR ETNYEPQLFL GFATDYSVGD WTLRDAEFGY NHQSNGRSDP TSRSWNRLYS 190 200 210 220 230 240 RLMAQNGNWL VEVKPWYVIG DTSDNKNITK YMGYYQLKIG YQLGEAVLSA KGQYNWNTGY 250 260 270 280 GGAELGVSYP ITKHVRFYTQ VYSGYGESLI DYDFNQTRVG MGVMLNDLF

References

[1]	"Molecular characterization of enterobacterial pldA genes encoding outer membrane phospholipase A." Brok R.G.P.M., Brinkman E., van Boxtel R., Bekkers A.C.A.P., Verheij H.M., Tommassen J. J. Bacteriol. 176:861-870(1994) [PubMed: 8300539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76902 Genomic DNA. Translation: CAA54224.1.
PIR	C36971.
3D structure databases
HSSP	HSSP built from PDB template 1QD6 based on UniProtKB P00631.
SMR	P37447. Positions 28-289.
ModBase	Search...
Family and domain databases
InterPro	IPR003187. PLA1. [Graphical view]
Gene3D	G3DSA:2.40.230.10. PLA1. 1 hit.
Pfam	PF02253. PLA1. 1 hit. [Graphical view]
PRINTS	PR01486. PHPHLIPASEA1.
ProtoNet	Search...

Entry information

Entry name

PA1_PROVU

Accession

Primary (citable) accession number: P37447

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 4, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)