Reviewed, UniProtKB/Swiss-Prot P37440 (UCPA_ECOLI)

Names and origin

Protein names

Recommended name:
    Oxidoreductase ucpA
    EC=1.-.-.-

Gene names

Name:	ucpA
Synonyms:	yfeF
Ordered Locus Names:	b2426, JW5394

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pfkA	P0A796	1	EBI-559387,EBI-554405

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Oxidoreductase ucpA		PRO_0000054801
Regions
	Nucleotide binding	10 – 32	23	NAD or NADP By similarity
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	141	1	Substrate By similarity
Experimental info
	Sequence conflict	153	1	T → Q in CAA68181. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37440-1 [UniParc]. Last modified July 11, 2001. Version 3. Checksum: 2E201713357FDF41	FASTA	263	27,850
	10 20 30 40 50 60 MGKLTGKTAL ITGALQGIGE GIARTFARHG ANLILLDISP EIEKLADELC GRGHRCTAVV 70 80 90 100 110 120 ADVRDPASVA AAIKRAKEKE GRIDILVNNA GVCRLGSFLD MSDDDRDFHI DINIKGVWNV 130 140 150 160 170 180 TKAVLPEMIA RKDGRIVMMS SVTGDMVADP GETAYALTKA AIVGLTKSLA VEYAQSGIRV 190 200 210 220 230 240 NAICPGYVRT PMAESIARQS NPEDPESVLT EMAKAIPMRR LADPLEVGEL AAFLASDESS 250 260 YLTGTQNVID GGSTLPETVS VGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the Escherichia coli gene encoding a new member of the short-chain dehydrogenase/reductase (SDR) family." Sirko A., Weglenska A., Hryniewicz M.M., Hulanicka D.M. Acta Biochim. Pol. 44:153-157(1997) [PubMed: 9241368] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Sulfate and thiosulfate transport in Escherichia coli K-12: identification of a gene encoding a novel protein involved in thiosulfate binding." Hryniewicz M.M., Sirko A., Palucha A., Boeck A., Hulanicka D.M. J. Bacteriol. 172:3358-3366(1990) [PubMed: 2188959] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 180-263. Strain: K12.
[6]	"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Borodovsky M., Rudd K.E., Koonin E.V. Nucleic Acids Res. 22:4756-4767(1994) [PubMed: 7984428] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99908 Genomic DNA. Translation: CAA68181.1. U00096 Genomic DNA. Translation: AAC75479.1. Different initiation. AP009048 Genomic DNA. Translation: BAA16309.2. M32101 Genomic DNA. No translation available.
PIR	A65017.
RefSeq	AP_003020.1. NP_416921.4.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11073N.
IntAct	P37440. 3 interactions.
STRING	P37440.
Genome annotation databases
GeneID	946898.
GenomeReviews	Gene locus JW5394 in contig AP009048_GR. Gene locus b2426 in contig U00096_GR.
KEGG	ecj:JW5394. eco:b2426.
Organism-specific databases
EchoBASE	EB2054.
EcoGene	EG12133. ucpA.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750976.
OMA	CRLADPL.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12133-MON. ECOL168927:B2426-MON.
Gene expression databases
Genevestigator	P37440.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCPA_ECOLI

Accession

Primary (citable) accession number: P37440
Secondary accession number(s): P76963, P77140, P77442

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 11, 2001
Last modified:	December 15, 2009
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)