PTS system glucose-specific EIICB component

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Reviewed, UniProtKB/Swiss-Prot P37439 (PTGCB_SALTY)

Last modified November 3, 2009. Version 80. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    PTS system glucose-specific EIICB component
Alternative name(s):
    EIICB-Glc
      Short name=EII-Glc
Including the following 2 domains:
    1- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    2- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component

Gene names

Name:	ptsG
Ordered Locus Names:	STM1203

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

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Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Miscellaneous	This enzyme is also a chemoreceptor monitoring the environment for changes in sugar concentration.
Sequence similarities	Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

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Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose transport Inferred from electronic annotation. Source: InterPro phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 477

477

PTS system glucose-specific EIICB component

PRO_0000186531

Regions

Topological domain

1 – 14

Cytoplasmic Potential

Transmembrane

15 – 35

Potential

Topological domain

36 – 50

Periplasmic Potential

Transmembrane

51 – 71

Potential

Topological domain

72 – 79

Cytoplasmic Potential

Transmembrane

80 – 100

Potential

Topological domain

101 – 111

Periplasmic Potential

Transmembrane

112 – 132

Potential

Topological domain

133 – 151

Cytoplasmic Potential

Transmembrane

152 – 172

Potential

Topological domain

173 – 190

Periplasmic Potential

Transmembrane

191 – 211

Potential

Topological domain

212 – 248

Cytoplasmic Potential

Transmembrane

249 – 269

Potential

Topological domain

270 – 279

Periplasmic Potential

Transmembrane

280 – 300

Potential

Topological domain

301 – 309

Cytoplasmic Potential

Transmembrane

310 – 330

Potential

Topological domain

331 – 355

Periplasmic Potential

Transmembrane

356 – 376

Potential

Topological domain

377 – 477

101

Cytoplasmic Potential

Domain

1 – 388

388

PTS EIIC type-1

Domain

399 – 477

PTS EIIB type-1

Sites

Active site

421

Phosphocysteine intermediate; for EIIB activity By similarity

Experimental info

Sequence conflict

218

F → S in CAA52702. Ref.1

Sequence conflict

379

L → F in CAA52702. Ref.1

Sequence conflict

477

S → H in CAA52702. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P37439-1 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 8CD10142542FF797
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FASTA

477

50,497

        10         20         30         40         50         60 
MFKNAFANLQ KVGKSLMLPV SVLPIAGILL GVGSANFSWL PAVVSHVMAE AGGSVFANMP 

        70         80         90        100        110        120 
LIFAIGVALG FTNNDGVSAL AAVVAYGIMV KTMAVVAPLV LHLPAEEIAA KHLADTGVLG 

       130        140        150        160        170        180 
GIISGAIAAY MFNRFYRIKL PEYLGFFAGK RFVPIISGLA AIFTGVVLSF VWPPIGTAIQ 

       190        200        210        220        230        240 
AFSQWAAYQN PVVAFGIYGF IERCLVPFGL HHIWNVPFQM QIGEYTNAAG QVFHGDIPRY 

       250        260        270        280        290        300 
MAGDPTAGML SGGFLFKMYG LPAAAIAIWH SAKPENRAKV GGIMISAALT SFLTGITEPI 

       310        320        330        340        350        360 
EFSFMFVAPI LYIIHAILAG LAFPICILLG MRDGTSFSHG LIDFIVLSGN SSKLWLFPIV 

       370        380        390        400        410        420 
GAGYAIVYYT VFRVLIKALD LKTPGREDTT DDAKAGATSE MAPALVAAFG GKENITNLDA 

       430        440        450        460        470 
CITRLRVSVA DVAKVDQAGL KKLGAAGVVV AGSGVQAIFG TKSDNLKTEM DEYIRNS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Stolz B., Wehrli C., Erni B. Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SB3507.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

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Cross-references

Sequence databases
	X74629 Genomic DNA. Translation: CAA52702.1. AE006468 Genomic DNA. Translation: AAL20132.1.
PIR	S36620.
RefSeq	NP_460173.1.
3D structure databases
HSSP	HSSP built from PDB template 1IBA based on UniProtKB P05053.
SMR	P37439. Positions 400-476.
ModBase	Search...
Proteomic databases
PRIDE	P37439.
Genome annotation databases
GeneID	1252721.
GenomeReviews	Gene locus STM1203 in contig AE006468_GR.
KEGG	stm:STM1203.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P37439.
OMA	NNDGVAG.
Enzyme and pathway databases
BioCyc	STYP99287:STM1203-MON.
BRENDA	2.7.1.69. 2.
Family and domain databases
InterPro	IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR004719. PTrfase_sys_maltose/Glc-sp_IIC. IPR001996. PTS_EIIB. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011299. PTS_IIBC_glc. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
Pfam	PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00852. pts-Glc. 1 hit. TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITE	PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

PTGCB_SALTY

Accession

Primary (citable) accession number: P37439

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2002
Last modified:	November 3, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents