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P37427

- RIR2_SALTY

UniProt

P37427 - RIR2_SALTY

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Protein
Ribonucleoside-diphosphate reductase 1 subunit beta
Gene
nrdB, STM2278
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron 1 By similarity
Metal bindingi116 – 1161Iron 1 By similarity
Metal bindingi116 – 1161Iron 2 By similarity
Metal bindingi119 – 1191Iron 1 By similarity
Active sitei123 – 1231 By similarity
Metal bindingi205 – 2051Iron 2 By similarity
Metal bindingi239 – 2391Iron 2 By similarity
Metal bindingi242 – 2421Iron 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
  2. deoxyribonucleoside diphosphate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2293-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase 1 subunit beta (EC:1.17.4.1)
Alternative name(s):
Protein B2
Protein R2
Ribonucleotide reductase 1
Gene namesi
Name:nrdB
Ordered Locus Names:STM2278
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 376375Ribonucleoside-diphosphate reductase 1 subunit beta
PRO_0000190478Add
BLAST

Proteomic databases

PaxDbiP37427.
PRIDEiP37427.

Interactioni

Subunit structurei

Tetramer of two alpha (R1) and two beta (R2) subunits. The B1 protein is a dimer of alpha subunits. A radical transfer pathway occurs between Tyr-123 of R2 and R1 By similarity.

Protein-protein interaction databases

STRINGi99287.STM2278.

Structurei

3D structure databases

ProteinModelPortaliP37427.
SMRiP37427. Positions 2-342.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000278087.
KOiK00526.
OMAiWIETWAF.
OrthoDBiEOG6J48J7.
PhylomeDBiP37427.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 2 hits.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37427-1 [UniParc]FASTAAdd to Basket

« Hide

MAYTTFSQTK NDQLKEPMFF GQPVNVARYD QQKYDIFEKL IEKQLSFFWR    50
PEEVDVSRDR IDYQALPEHE KHIFISNLKY QTLLDSIQGR SPNVALLPLI 100
SIPELETWVE TWAFSETIHS RSYTHIIRNI VNDPAVVFDD IVTNEQIQKR 150
AEGISAYYDE LIEMTSYWHL LGEGTHTVNG KTVVVNLREL KKKLYLCLMS 200
VNALEAIRFY VSFACSFAFA ERELMEGNAK IIRLIARDEA LHLTGTQHML 250
NLLRSGVDDP EMAEIAEECK QECYDLFVQA AQQEKEWADY LFRDGSMIGL 300
NKDILCQYVE YITNIRMQAV GLDLPFQTRS NPIPWINTWL VSDNVQVAPQ 350
EVEVSSYLVG QIDSEVDTDD LSNFQL 376
Length:376
Mass (Da):43,552
Last modified:January 23, 2007 - v3
Checksum:iA82E9F1ABE058226
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL21179.1.
X72948 Genomic DNA. Translation: CAA51453.1.
PIRiS32630.
RefSeqiNP_461220.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21179; AAL21179; STM2278.
GeneIDi1253800.
KEGGistm:STM2278.
PATRICi32383163. VBISalEnt20916_2411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL21179.1 .
X72948 Genomic DNA. Translation: CAA51453.1 .
PIRi S32630.
RefSeqi NP_461220.1. NC_003197.1.

3D structure databases

ProteinModelPortali P37427.
SMRi P37427. Positions 2-342.
ModBasei Search...

Protein-protein interaction databases

STRINGi 99287.STM2278.

Proteomic databases

PaxDbi P37427.
PRIDEi P37427.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL21179 ; AAL21179 ; STM2278 .
GeneIDi 1253800.
KEGGi stm:STM2278.
PATRICi 32383163. VBISalEnt20916_2411.

Phylogenomic databases

eggNOGi COG0208.
HOGENOMi HOG000278087.
KOi K00526.
OMAi WIETWAF.
OrthoDBi EOG6J48J7.
PhylomeDBi P37427.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci SENT99287:GCTI-2293-MONOMER.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 2 hits.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase."
    Jordan A., Gibert I., Barbe J.
    J. Bacteriol. 176:3420-3427(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-122.
  3. Jordan A.
    Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-376.

Entry informationi

Entry nameiRIR2_SALTY
AccessioniPrimary (citable) accession number: P37427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A substrate-binding catalytic site, located on R1, is formed only in the presence of the second subunit R2 By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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