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P37427

- RIR2_SALTY

UniProt

P37427 - RIR2_SALTY

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Protein

Ribonucleoside-diphosphate reductase 1 subunit beta

Gene

nrdB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron 1PROSITE-ProRule annotation
Metal bindingi116 – 1161Iron 1PROSITE-ProRule annotation
Metal bindingi116 – 1161Iron 2By similarity
Metal bindingi119 – 1191Iron 1PROSITE-ProRule annotation
Active sitei123 – 1231PROSITE-ProRule annotation
Metal bindingi205 – 2051Iron 2By similarity
Metal bindingi239 – 2391Iron 2By similarity
Metal bindingi242 – 2421Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2293-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase 1 subunit beta (EC:1.17.4.1)
Alternative name(s):
Protein B2
Protein R2
Ribonucleotide reductase 1
Gene namesi
Name:nrdB
Ordered Locus Names:STM2278
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 376375Ribonucleoside-diphosphate reductase 1 subunit betaPRO_0000190478Add
BLAST

Proteomic databases

PaxDbiP37427.
PRIDEiP37427.

Interactioni

Subunit structurei

Tetramer of two alpha (R1) and two beta (R2) subunits. The B1 protein is a dimer of alpha subunits. A radical transfer pathway occurs between Tyr-123 of R2 and R1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi99287.STM2278.

Structurei

3D structure databases

ProteinModelPortaliP37427.
SMRiP37427. Positions 2-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000278087.
KOiK00526.
OMAiWIETWAF.
OrthoDBiEOG6J48J7.
PhylomeDBiP37427.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 2 hits.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37427-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYTTFSQTK NDQLKEPMFF GQPVNVARYD QQKYDIFEKL IEKQLSFFWR
60 70 80 90 100
PEEVDVSRDR IDYQALPEHE KHIFISNLKY QTLLDSIQGR SPNVALLPLI
110 120 130 140 150
SIPELETWVE TWAFSETIHS RSYTHIIRNI VNDPAVVFDD IVTNEQIQKR
160 170 180 190 200
AEGISAYYDE LIEMTSYWHL LGEGTHTVNG KTVVVNLREL KKKLYLCLMS
210 220 230 240 250
VNALEAIRFY VSFACSFAFA ERELMEGNAK IIRLIARDEA LHLTGTQHML
260 270 280 290 300
NLLRSGVDDP EMAEIAEECK QECYDLFVQA AQQEKEWADY LFRDGSMIGL
310 320 330 340 350
NKDILCQYVE YITNIRMQAV GLDLPFQTRS NPIPWINTWL VSDNVQVAPQ
360 370
EVEVSSYLVG QIDSEVDTDD LSNFQL
Length:376
Mass (Da):43,552
Last modified:January 23, 2007 - v3
Checksum:iA82E9F1ABE058226
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL21179.1.
X72948 Genomic DNA. Translation: CAA51453.1.
PIRiS32630.
RefSeqiNP_461220.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21179; AAL21179; STM2278.
GeneIDi1253800.
KEGGistm:STM2278.
PATRICi32383163. VBISalEnt20916_2411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL21179.1 .
X72948 Genomic DNA. Translation: CAA51453.1 .
PIRi S32630.
RefSeqi NP_461220.1. NC_003197.1.

3D structure databases

ProteinModelPortali P37427.
SMRi P37427. Positions 2-342.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM2278.

Proteomic databases

PaxDbi P37427.
PRIDEi P37427.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL21179 ; AAL21179 ; STM2278 .
GeneIDi 1253800.
KEGGi stm:STM2278.
PATRICi 32383163. VBISalEnt20916_2411.

Phylogenomic databases

eggNOGi COG0208.
HOGENOMi HOG000278087.
KOi K00526.
OMAi WIETWAF.
OrthoDBi EOG6J48J7.
PhylomeDBi P37427.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci SENT99287:GCTI-2293-MONOMER.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 2 hits.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase."
    Jordan A., Gibert I., Barbe J.
    J. Bacteriol. 176:3420-3427(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-122.
  3. Jordan A.
    Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-376.

Entry informationi

Entry nameiRIR2_SALTY
AccessioniPrimary (citable) accession number: P37427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A substrate-binding catalytic site, located on R1, is formed only in the presence of the second subunit R2.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3