Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P37427 (RIR2_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase 1 subunit beta

EC=1.17.4.1
Alternative name(s):
Protein B2
Protein R2
Ribonucleotide reductase 1
Gene names
Name:nrdB
Ordered Locus Names:STM2278
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Tetramer of two alpha (R1) and two beta (R2) subunits. The B1 protein is a dimer of alpha subunits. A radical transfer pathway occurs between Tyr-123 of R2 and R1 By similarity.

Miscellaneous

A substrate-binding catalytic site, located on R1, is formed only in the presence of the second subunit R2 By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 376375Ribonucleoside-diphosphate reductase 1 subunit beta
PRO_0000190478

Sites

Active site1231 By similarity
Metal binding851Iron 1 By similarity
Metal binding1161Iron 1 By similarity
Metal binding1161Iron 2 By similarity
Metal binding1191Iron 1 By similarity
Metal binding2051Iron 2 By similarity
Metal binding2391Iron 2 By similarity
Metal binding2421Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P37427 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: A82E9F1ABE058226

FASTA37643,552
        10         20         30         40         50         60 
MAYTTFSQTK NDQLKEPMFF GQPVNVARYD QQKYDIFEKL IEKQLSFFWR PEEVDVSRDR 

        70         80         90        100        110        120 
IDYQALPEHE KHIFISNLKY QTLLDSIQGR SPNVALLPLI SIPELETWVE TWAFSETIHS 

       130        140        150        160        170        180 
RSYTHIIRNI VNDPAVVFDD IVTNEQIQKR AEGISAYYDE LIEMTSYWHL LGEGTHTVNG 

       190        200        210        220        230        240 
KTVVVNLREL KKKLYLCLMS VNALEAIRFY VSFACSFAFA ERELMEGNAK IIRLIARDEA 

       250        260        270        280        290        300 
LHLTGTQHML NLLRSGVDDP EMAEIAEECK QECYDLFVQA AQQEKEWADY LFRDGSMIGL 

       310        320        330        340        350        360 
NKDILCQYVE YITNIRMQAV GLDLPFQTRS NPIPWINTWL VSDNVQVAPQ EVEVSSYLVG 

       370 
QIDSEVDTDD LSNFQL 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
[2]"Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase."
Jordan A., Gibert I., Barbe J.
J. Bacteriol. 176:3420-3427(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-122.
[3]Jordan A.
Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-376.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006468 Genomic DNA. Translation: AAL21179.1.
X72948 Genomic DNA. Translation: CAA51453.1.
PIRS32630.
RefSeqNP_461220.1. NC_003197.1.

3D structure databases

ProteinModelPortalP37427.
SMRP37427. Positions 2-342.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM2278.

Proteomic databases

PaxDbP37427.
PRIDEP37427.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL21179; AAL21179; STM2278.
GeneID1253800.
KEGGstm:STM2278.
PATRIC32383163. VBISalEnt20916_2411.

Phylogenomic databases

eggNOGCOG0208.
HOGENOMHOG000278087.
KOK00526.
OMAWIETWAF.
OrthoDBEOG6J48J7.
PhylomeDBP37427.

Enzyme and pathway databases

BioCycSENT99287:GCTI-2293-MONOMER.
UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 2 hits.
[Graphical view]
SUPFAMSSF47240. SSF47240. 1 hit.
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR2_SALTY
AccessionPrimary (citable) accession number: P37427
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways