Reviewed, UniProtKB/Swiss-Prot P37416 (BIRA_SALTY)

Names and origin

Protein names

Recommended name:
    Bifunctional protein birA
Including the following 2 domains:
    1- Recommended name:
            Biotin operon repressor
    2- Recommended name:
            Biotin--[acetyl-CoA-carboxylase] synthetase
              EC=6.3.4.15
        Alternative name(s):
            Biotin--protein ligase

Gene names

Name:	birA
Ordered Locus Names:	STM4138
ORF Names:	STMF1.2

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins.
Catalytic activity	ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].
Subunit structure	Monomer.
Sequence similarities	Belongs to the biotin--protein ligase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	ATP-binding Biotin DNA-binding Nucleotide-binding
Molecular function	Ligase Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW biotin-[acetyl-CoA-carboxylase] ligase activity Inferred from electronic annotation. Source: EC transcription repressor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Bifunctional protein birA		PRO_0000064935
Regions
	DNA binding	22 – 41	20	H-T-H motif By similarity
Experimental info
	Sequence conflict	100 – 101	2	EL → DV Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P37416-1 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 010E8483093297F5	FASTA	320	35,395
	10 20 30 40 50 60 MKDTTVPLTL ISLLADGEFH SGEQLGERLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL 70 80 90 100 110 120 PEPIQLLDAD RIHSQLDSGN VAVLPVIDST NQYLLDRIGE LRSGDACVAE YQQAGRGRRG 130 140 150 160 170 180 RKWFSPFGAN LYLSMYWRLE QGPAAAIGLS LVIGIVMAEV LRKLGADKVR VKWPNDLYLL 190 200 210 220 230 240 DRKLAGILVE LTGKTGDAAQ IVIGAGINMA MRRVEEDVIN QGWITLQEAG ITLDRNMLAA 250 260 270 280 290 300 KLIYKLRAAL ELFEQEGLSP YLSRWKKLDN FIDRPVKLII GDKEIFGISR GIDTQGALLL 310 320 EQDGVIKPWM GGEISLRSAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[2]	"Sequence divergence of the murB and rrfB genes from Escherichia coli and Salmonella typhimurium." Dombrosky P.M., Schmid M.B., Young K.D. Arch. Microbiol. 161:501-507(1994) [PubMed: 8048842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-135. Strain: LT2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF170176 Genomic DNA. Translation: AAF33493.1. AE006468 Genomic DNA. Translation: AAL22971.1. L14816 Genomic DNA. No translation available.
RefSeq	NP_463012.1.
3D structure databases
HSSP	HSSP built from PDB template 1BIA based on UniProtKB P06709.
SMR	P37416. Positions 3-320.
ModBase	Search...
Proteomic databases
PRIDE	P37416.
Genome annotation databases
GeneID	1255664.
GenomeReviews	Gene locus STM4138 in contig AE006468_GR.
KEGG	stm:STM4138.
NMPDR	fig|99287.1.peg.3985.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P37416.
OMA	GTGIWMS.
Enzyme and pathway databases
BioCyc	STYP99287:STM4138-MON.
BRENDA	6.3.4.15. 2.
Family and domain databases
InterPro	IPR004408. Biotin_CoA_COase_ligase. IPR003142. BPL_C. IPR004143. BPL_LipA_LipB. IPR013196. HTH_11. IPR004409. HTHbiotin_repres. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR12835. BirA_ligase. 1 hit.
Pfam	PF02237. BPL_C. 1 hit. PF03099. BPL_LipA_LipB. 1 hit. PF08279. HTH_11. 1 hit. [Graphical view]
TIGRFAMs	TIGR00121. birA_ligase. 1 hit. TIGR00122. birA_repr_reg. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIRA_SALTY

Accession

Primary (citable) accession number: P37416
Secondary accession number(s): Q9L9K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	August 29, 2001
Last modified:	November 3, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)