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Protein

Bifunctional ligase/repressor BirA

Gene

birA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.UniRule annotation

Catalytic activityi

ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112BiotinUniRule annotation1
Binding sitei183BiotinUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi22 – 41H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Ligase, Repressor
Biological processTranscription, Transcription regulation
LigandATP-binding, Biotin, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ligase/repressor BirAUniRule annotation
Alternative name(s):
Biotin operon repressorUniRule annotation
Biotin--[acetyl-CoA-carboxylase] ligaseUniRule annotation (EC:6.3.4.15UniRule annotation)
Biotin--protein ligaseUniRule annotation
Biotin-[acetyl-CoA carboxylase] synthetaseUniRule annotation
Gene namesi
Name:birAUniRule annotation
Ordered Locus Names:STM4138
ORF Names:STMF1.2
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000649351 – 320Bifunctional ligase/repressor BirAAdd BLAST320

Proteomic databases

PaxDbiP37416.
PRIDEiP37416.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM4138.

Structurei

3D structure databases

ProteinModelPortaliP37416.
SMRiP37416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 254BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST189

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni89 – 91Biotin bindingUniRule annotation3
Regioni116 – 118Biotin bindingUniRule annotation3

Sequence similaritiesi

Belongs to the biotin--protein ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105HJX. Bacteria.
COG0340. LUCA.
COG1654. LUCA.
HOGENOMiHOG000041812.
KOiK03524.
OMAiRAAVWKH.
PhylomeDBiP37416.

Family and domain databases

CDDicd16442. BPL. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00978. Bifunct_BirA. 1 hit.
InterProiView protein in InterPro
IPR030855. Bifunct_BirA.
IPR004408. Biotin_CoA_COase_ligase.
IPR004409. Biotin_operon_repress_HTH.
IPR003142. BPL_C.
IPR004143. BPL_LPL_catalytic.
IPR013196. HTH_11.
IPR008988. Transcriptional_repressor_C.
IPR036388. WH-like_DNA-bd_sf.
IPR036390. WH_DNA-bd_sf.
PANTHERiPTHR12835:SF5. PTHR12835:SF5. 1 hit.
PfamiView protein in Pfam
PF02237. BPL_C. 1 hit.
PF03099. BPL_LplA_LipB. 1 hit.
PF08279. HTH_11. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50037. SSF50037. 1 hit.
TIGRFAMsiTIGR00121. birA_ligase. 1 hit.
TIGR00122. birA_repr_reg. 1 hit.
PROSITEiView protein in PROSITE
PS51733. BPL_LPL_CATALYTIC. 1 hit.

Sequencei

Sequence statusi: Complete.

P37416-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDTTVPLTL ISLLADGEFH SGEQLGERLG MSRAAINKHI QTLRDWGVDV
60 70 80 90 100
FTVPGKGYSL PEPIQLLDAD RIHSQLDSGN VAVLPVIDST NQYLLDRIGE
110 120 130 140 150
LRSGDACVAE YQQAGRGRRG RKWFSPFGAN LYLSMYWRLE QGPAAAIGLS
160 170 180 190 200
LVIGIVMAEV LRKLGADKVR VKWPNDLYLL DRKLAGILVE LTGKTGDAAQ
210 220 230 240 250
IVIGAGINMA MRRVEEDVIN QGWITLQEAG ITLDRNMLAA KLIYKLRAAL
260 270 280 290 300
ELFEQEGLSP YLSRWKKLDN FIDRPVKLII GDKEIFGISR GIDTQGALLL
310 320
EQDGVIKPWM GGEISLRSAE
Length:320
Mass (Da):35,395
Last modified:August 29, 2001 - v2
Checksum:i010E8483093297F5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100 – 101EL → DV (PubMed:8048842).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF170176 Genomic DNA. Translation: AAF33493.1.
AE006468 Genomic DNA. Translation: AAL22971.1.
L14816 Genomic DNA. No translation available.
RefSeqiNP_463012.1. NC_003197.2.
WP_000655752.1. NC_003197.2.

Genome annotation databases

EnsemblBacteriaiAAL22971; AAL22971; STM4138.
GeneIDi1255664.
KEGGistm:STM4138.
PATRICifig|99287.12.peg.4354.

Similar proteinsi

Entry informationi

Entry nameiBIRA_SALTY
AccessioniPrimary (citable) accession number: P37416
Secondary accession number(s): Q9L9K4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: August 29, 2001
Last modified: October 25, 2017
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families