P37411 (GYRA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	STM2272

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Miscellaneous	When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA. HAMAP MF_01897
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145248
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37411 [UniParc]. Last modified December 13, 2001. Version 3. Checksum: CD6F5582190AE53C	FASTA	878	97,064
	10 20 30 40 50 60 MSDLAREITP VNIEEELKSS YLDYAMSVIV GRALPDVRDG LKPVHRRVLY AMNVLGNDWN 70 80 90 100 110 120 KAYKKSARVV GDVIGKYHPH GDSAVYDTIV RMAQPFSLRY MLVDGQGNFG SIDGDSAAAM 130 140 150 160 170 180 RYTEIRLAKI AHELMADLEK ETVDFVDNYD GTEKIPDVMP TKIPNLLVNG SSGIAVGMAT 190 200 210 220 230 240 NIPPHNLTEV INGCLAYIDN EDISIEGLME HIPGPDFPTA AIINGRRGIE EAYRTGRGKV 250 260 270 280 290 300 YIRARAEVEA DAKTGRETII VHEIPYQVNK ARLIEKIAEL VKDKRVEGIS ALRDESDKDG 310 320 330 340 350 360 MRIVIEVKRD AVGEVVLNNL YSQTQLQVSF GINMVALHHG QPKIMNLKDI ISAFVRHRRE 370 380 390 400 410 420 VVTRRTIFEL RKARDRAHIL EALAIALANI DPIIELIRRA PTPAEAKAAL ISRPWDLGNV 430 440 450 460 470 480 AAMLERAGDD AARPEWLEPE FGVRDGQYYL TEQQAQAILD LRLQKLTGLE HEKLLDEYKE 490 500 510 520 530 540 LLEQIAELLH ILGSADRLME VIREEMELIR DQFGDERRTE ITANSADINI EDLISQEDVV 550 560 570 580 590 600 VTLSHQGYVK YQPLTDYEAQ RRGGKGKSAA RIKEEDFIDR LLVANTHDTI LCFSSRGRLY 610 620 630 640 650 660 WMKVYQLPEA SRGARGRPIV NLLPLEANER ITAILPVREY EEGVNVFMAT ASGTVKKTAL 670 680 690 700 710 720 TEFSRPRSAG IIAVNLNDGD ELIGVDLTSG SDEVMLFSAA GKVVRFKEDA VRAMGRTATG 730 740 750 760 770 780 VRGIKLAGDD KVVSLIIPRG EGAILTVTQN GYGKRTAADE YPTKSRATQG VISIKVTERN 790 800 810 820 830 840 GSVVGAVQVD DCDQIMMITD AGTLVRTRVS EISVVGRNTQ GVILIRTAED ENVVGLQRVA 850 860 870 EPVDDEELDA IDGSVAEGDE DIAPEAESDD DVADDADE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[2]	Griggs D.J. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 39-160. Strain: NCTC 74.
[3]	Ruiz J., Castro D., Goni P., Borrego J.J., Vila J. Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-166. Strain: SM4.
[4]	"Mutations in gyrA gene of quinolone-resistant Salmonella serotypes isolated from humans and animals." Griggs D., Gensberg K., Piddock L.J.V. Antimicrob. Agents Chemother. 40:1009-1013(1996) [PubMed: 8849216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 74-159. Strain: NCTC 74.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL21173.1. X78977 Genomic DNA. Translation: CAA55580.1. X86695 Genomic DNA. Translation: CAA60388.1.
PIR	S54254.
RefSeq	NP_461214.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P37411.
SMR	P37411. Positions 30-522, 535-841.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1253794.
GenomeReviews	Gene locus STM2272 in contig AE006468_GR.
KEGG	stm:STM2272.
PATRIC	32383151. VBISalEnt20916_2405.
Phylogenomic databases
HOGENOM	HBG635183.
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	STYP99287:STM2272-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_SALTY

Accession

Primary (citable) accession number: P37411

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	December 13, 2001
Last modified:	January 25, 2012
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program