Probable cadmium-transporting ATPase

Preferred order of sections

Names and origin

Protein names

Recommended name:
Probable cadmium-transporting ATPase
EC=3.6.3.3

Alternative name(s):
Cadmium efflux ATPase

Gene names

Name:

cadA

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	804 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism By similarity.
Catalytic activity	ATP + H₂O + Cd²⁺(In) = ADP + phosphate + Cd²⁺(Out).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. [View classification] Contains 2 HMA domains.

Ontologies

Keywords
Biological process	Antiport Cadmium resistance Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Cadmium Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Transposable element
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW antiporter activity Inferred from electronic annotation. Source: UniProtKB-KW cadmium-exporting ATPase activity Inferred from electronic annotation. Source: EC cation channel activity Inferred from electronic annotation. Source: InterPro copper-exporting ATPase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 804

804

Probable cadmium-transporting ATPase

PRO_0000046249

Regions

Transmembrane

183 – 203

21

Helical; Potential

Transmembrane

207 – 227

21

Helical; Potential

Transmembrane

248 – 268

21

Helical; Potential

Transmembrane

413 – 433

21

Helical; Potential

Transmembrane

441 – 461

21

Helical; Potential

Transmembrane

749 – 771

23

Helical; Potential

Transmembrane

776 – 798

23

Helical; Potential

Domain

12 – 91

80

HMA 1

Domain

90 – 153

64

HMA 2

Sites

Active site

492

1

4-aspartylphosphate intermediate Probable

Metal binding

22

1

Cadmium Potential

Metal binding

25

1

Cadmium Potential

Metal binding

100

1

Cadmium Potential

Metal binding

103

1

Cadmium Potential

Natural variations

Natural variant

135

1

A → T in strain: 85/2082.

Sequences

Sequence

Length

Mass (Da)

Tools

P37386 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: D8D26DE6EB878026
Show »

FASTA

804

86,883

        10         20         30         40         50         60 
MDSSTKTLTE DKQVYRVEGF SCANCAGKFE KNVKELSGVH DAKVNFGASK IDVFGSATVE 

        70         80         90        100        110        120 
DLEKAGAFEN LKVAPEKARR RVEPVVTEDK NVYRVEGFSC ANCAGKFEKN VKQLAGVQDA 

       130        140        150        160        170        180 
KVNFGASKID VYGNASVEEL EKAGAFENLK VIPEKLANPS IQAVKEDTKA PKEEKIPFYK 

       190        200        210        220        230        240 
KHSTLLFATL LIAFGYLSHF VNGEDNLVTS MLFVSSIVIG GYSLFKVGFQ NLIRFDFDMK 

       250        260        270        280        290        300 
TLMTVAVIGA AIIGEWAEAS IVVILFAISE ALERFSMDRA RQSIRSLMDI APKEALVRRN 

       310        320        330        340        350        360 
GQEIMIHVDD IAVGDIMIVK PGEKIAMDGI IINGVSAVNQ AAITGESVPV AKTVDDEVFA 

       370        380        390        400        410        420 
GTLNEEGLLE VKITKYVEDT TISKIIHLVE EAQGERAPAQ AFVDKFAKYY TPIIMVIAAL 

       430        440        450        460        470        480 
VAVVPPLFFG GSWDTWVYQG LAVLVVGCPC ALVITTPISI VSAIGNAAKK GVLIKGGVYL 

       490        500        510        520        530        540 
EELGAIKAIA FDKTGTLTKG VPVVTDFKVL NDQVEEKELF SIITALEYRS QHPLASAIMK 

       550        560        570        580        590        600 
KAEQDNITYS DVRVKDFTSI TGRGIQGNID GTTYYIGSPR LFKELNVSDF SLEFENKVKV 

       610        620        630        640        650        660 
LQNQGKTAMI IGTDQTILGV IAVADEVRET SKNVILKLHQ LGIKQTIMLT GDNQGTAEAI 

       670        680        690        700        710        720 
GAHVGVSDIQ SELLPQDKLD YIKKMKAEHG NVAMIGDGVN DAPALAASTV GIAMGGAGTD 

       730        740        750        760        770        780 
TAIETADIAL MGDDLSKLPF AVRLSRKTLN IIKANITFAI GIKIIALLLV IPGWLTLWIA 

       790        800 
ILSDMGATIL VALNSLRLMR VKDK

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References

[1]	"PsiTn554: a Staphylococcus aureus chromosomal element encoding cadmium resistance determinants, and genes resembling the transposases genes of Tn554." Chikramane S.G., Dubin D.T. Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Transposon: PsiTn554.
[2]	"Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus." Ito T., Katayama Y., Asada K., Mori N., Tsutsumimoto K. Antimicrob. Agents Chemother. 45:1323-1336(2001) [PubMed: 11302791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 85/2082 and 85/3907.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10909 Genomic DNA. Translation: AAA26610.1. AB047089 Genomic DNA. Translation: BAC57487.1. AB037671 Genomic DNA. Translation: BAB47609.1.
3D structure databases
ProteinModelPortal	P37386.
SMR	P37386. Positions 11-77, 89-157.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR006403. ATPase_P-typ_cat/Cu-transptr. IPR001366. ATPase_P-typ_Cd-transp. IPR006404. ATPase_P-typ_Cd/Co/Hg/Pb/Zn. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR006416. ATPase_P-typ_heavy-metal. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. IPR017969. Heavy-metal-associated_CS. IPR006121. HeavyMe-assoc_HMA. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit. G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00403. HMA. 2 hits. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00941. CDATPASE.
SUPFAM	SSF56784. HAD-like_dom. 1 hit. SSF55008. HeavyMe_transpt. 2 hits.
TIGRFAMs	TIGR01511. ATPase-IB1_Cu. 1 hit. TIGR01512. ATPase-IB2_Cd. 1 hit. TIGR01525. ATPase-IB_hvy. 1 hit. TIGR01494. ATPase_P-type. 1 hit.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. PS01047. HMA_1. 2 hits. PS50846. HMA_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CADA2_STAAU

Accession

Primary (citable) accession number: P37386
Secondary accession number(s): Q7DIB0, Q93ID4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	September 21, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

Entry information

Relevant documents