Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-directed RNA polymerase II subunit RPB3

Gene

rpb3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • transcription by RNA polymerase II Source: PomBase

Keywordsi

Biological processTranscription

Enzyme and pathway databases

ReactomeiR-SPO-113418 Formation of the Early Elongation Complex
R-SPO-5578749 Transcriptional regulation by small RNAs
R-SPO-674695 RNA Polymerase II Pre-transcription Events
R-SPO-6781823 Formation of TC-NER Pre-Incision Complex
R-SPO-6782135 Dual incision in TC-NER
R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SPO-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SPO-72086 mRNA Capping
R-SPO-72165 mRNA Splicing - Minor Pathway
R-SPO-73776 RNA Polymerase II Promoter Escape
R-SPO-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SPO-75953 RNA Polymerase II Transcription Initiation
R-SPO-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SPO-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-SPO-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB3
Short name:
RNA polymerase II subunit 3
Short name:
RNA polymerase II subunit B3
Alternative name(s):
DNA-directed RNA polymerase II 33 kDa polypeptide
Gene namesi
Name:rpb3
ORF Names:SPCC1442.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1442.10c
PomBaseiSPCC1442.10c rpb3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001327471 – 297DNA-directed RNA polymerase II subunit RPB3Add BLAST297

Proteomic databases

MaxQBiP37382
PaxDbiP37382
PRIDEiP37382

PTM databases

iPTMnetiP37382

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi275397, 23 interactors
IntActiP37382, 4 interactors
MINTiP37382
STRINGi4896.SPCC1442.10c.1

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65C/O1-297[»]
5U0Selectron microscopy7.80c1-297[»]
ProteinModelPortaliP37382
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37382

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000230844
InParanoidiP37382
KOiK03011
OMAiMAIDWVQ
OrthoDBiEOG092C3EZ0
PhylomeDBiP37382

Family and domain databases

Gene3Di2.170.120.12, 1 hit
3.30.1360.10, 2 hits
InterProiView protein in InterPro
IPR001514 DNA-dir_RNA_pol_30-40kDasu_CS
IPR011262 DNA-dir_RNA_pol_insert
IPR011263 DNA-dir_RNA_pol_RpoA/D/Rpb3
IPR036603 RBP11-like
IPR036643 RNApol_insert_sf
PfamiView protein in Pfam
PF01000 RNA_pol_A_bac, 1 hit
PF01193 RNA_pol_L, 1 hit
SMARTiView protein in SMART
SM00662 RPOLD, 1 hit
SUPFAMiSSF55257 SSF55257, 2 hits
SSF56553 SSF56553, 1 hit
PROSITEiView protein in PROSITE
PS00446 RNA_POL_D_30KD, 1 hit

Sequencei

Sequence statusi: Complete.

P37382-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSETHITIR NISKNSVDFV LTNTSLAVAN SLRRVVLAEI PTVAIDLVEI
60 70 80 90 100
NVNTSVMPDE FLAHRLGMIP LDSSNIDEPP PVGLEYTRNC DCDQYCPKCS
110 120 130 140 150
VELFLNAKCT GEGTMEIYAR DLVVSSNSSL GHPILADPKS RGPLICKLRK
160 170 180 190 200
EQEISLRCIA KKGIAKEHAK WSPTSAVAFE YDPWNKLQHT DYWFENDADA
210 220 230 240 250
EWPKSKNADW EEPPREGEPF NFQEEPRRFY MDVESVGSIP PNEIMVQGLR
260 270 280 290
ILQEKLAVLV RDLDEEQPTQ LSANELNMEE NAEMNWSPYQ NGEENTW
Length:297
Mass (Da):33,716
Last modified:February 21, 2001 - v2
Checksum:i94F639734B162DDF
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti7I → L in strain: Isolate TS99. 1
Natural varianti14 – 15KN → RD in strain: Isolate TS54. 2
Natural varianti24T → S in strain: Isolate TS54. 1
Natural varianti29A → P in strain: Isolate TS99. 1
Natural varianti67G → D in strain: Isolate TS54. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15070 Genomic DNA Translation: BAA22566.1
CU329672 Genomic DNA Translation: CAA21444.1
D50661 Genomic DNA Translation: BAA09315.1
D50662 Genomic DNA Translation: BAA09316.1
PIRiS43201
T40975
RefSeqiNP_588324.1, NM_001023315.2

Genome annotation databases

EnsemblFungiiSPCC1442.10c.1; SPCC1442.10c.1:pep; SPCC1442.10c
GeneIDi2538816
KEGGispo:SPCC1442.10c

Similar proteinsi

Entry informationi

Entry nameiRPB3_SCHPO
AccessioniPrimary (citable) accession number: P37382
Secondary accession number(s): P78959, P78960, Q9UUM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 21, 2001
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health