Reviewed, UniProtKB/Swiss-Prot P37361 (MT3_RAT)

Names and origin

Protein names

Recommended name:
    Metallothionein-3
      Short name=MT-3
Alternative name(s):
    Metallothionein-III
      Short name=MT-III
    Growth inhibitory factor
      Short name=GIF

Gene names

Name:

Mt3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	66 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro By similarity.
Tissue specificity	Brain.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Copper Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Gene Ontology (GO)
Biological process	negative regulation of cell growth Ref.2 Non-traceable author statement. Source: UniProtKB negative regulation of dendrite morphogenesis Non-traceable author statement. Source: UniProtKB removal of superoxide radicals Inferred from sequence or structural similarity. Source: UniProtKB response to hypoxia Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	synaptic vesicle Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 66	66	Metallothionein-3		PRO_0000197253
Regions
	Region	1 – 30	30	Beta
	Region	31 – 66	36	Alpha
Sites
	Metal binding	6	1	Cluster B By similarity
	Metal binding	8	1	Cluster B By similarity
	Metal binding	14	1	Cluster B By similarity
	Metal binding	16	1	Cluster B By similarity
	Metal binding	20	1	Cluster B By similarity
	Metal binding	22	1	Cluster B By similarity
	Metal binding	25	1	Cluster B By similarity
	Metal binding	27	1	Cluster B By similarity
	Metal binding	30	1	Cluster B By similarity
	Metal binding	34	1	Cluster A By similarity
	Metal binding	35	1	Cluster A By similarity
	Metal binding	37	1	Cluster A By similarity
	Metal binding	38	1	Cluster A By similarity
	Metal binding	42	1	Cluster A By similarity
	Metal binding	45	1	Cluster A By similarity
	Metal binding	49	1	Cluster A By similarity
	Metal binding	51	1	Cluster A By similarity
	Metal binding	62	1	Cluster A By similarity
	Metal binding	64	1	Cluster A By similarity
	Metal binding	65	1	Cluster A By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37361-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: BE7538E85664EBF8	FASTA	66	6,809
	10 20 30 40 50 60 MDPETCPCPT GGSCTCSDKC KCKGCKCTNC KKSCCSCCPA GCEKCAKDCV CKGEEGAKAE KCSCCQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of rat growth inhibitory factor cDNA and the expression in the central nervous system." Kobayashi H., Uchida Y., Ihara Y., Nakajima K., Kohsaka S., Miyatake T., Tsuji S. Brain Res. Mol. Brain Res. 19:188-194(1993) [PubMed: 8412560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Modulation of metallothionein-III mRNA content and growth rate of rat C6-glial cells by transfection with human 5-HT1D receptor genes." Amoureux M.C., Wurch T., Pauwels P.J. Biochem. Biophys. Res. Commun. 214:639-645(1995) [PubMed: 7677777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Glial tumor.
[3]	"Structural and functional analysis of the rat metallothionein III genomic locus." Chapman G.A., Kay J., Kille P. Biochim. Biophys. Acta 1445:321-329(1999) [PubMed: 10366715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S65838 mRNA. Translation: AAB28366.1. X89603 mRNA. Translation: CAA61762.1. Y08235 Genomic DNA. Translation: CAA69404.1. BC058453 mRNA. Translation: AAH58453.1.
IPI	IPI00205463.
PIR	S58086.
RefSeq	NP_446420.1.
UniGene	Rn.11325
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025669; ENSRNOP00000025669; ENSRNOG00000018958; Rattus norvegicus. [Genome view]
GeneID	117038.
KEGG	rno:117038.
UCSC	NM_053968. rat.
Organism-specific databases
CTD	117038.
RGD	621252. Mt3.
Phylogenomic databases
HOVERGEN	P37361.
InParanoid	P37361.
OMA	ETCPCPT.
Gene expression databases
ArrayExpress	P37361.
Genevestigator	P37361.
GermOnline	ENSRNOG00000018958. Rattus norvegicus.
Family and domain databases
InterPro	IPR017854. Metallothionein_sfam. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. IPR018064. Metallothionein_vert_metal_BS. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	619839.

Entry information

Entry name

MT3_RAT

Accession

Primary (citable) accession number: P37361

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 15, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)