P37360 (MT3_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metallothionein-3
Short name=MT-3

Alternative name(s):
Growth inhibitory factor
Short name=GIF
Metallothionein-III
Short name=MT-III

Gene names

Name:

MT3

Organism

Equus caballus (Horse) [Reference proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › Equus

Protein attributes

Sequence length	68 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium.
Tissue specificity	Brain.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Copper Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ERK1 and ERK2 cascade Inferred from sequence or structural similarity. Source: UniProtKB activation of protein kinase B activity Inferred from sequence or structural similarity. Source: UniProtKB astrocyte development Inferred from sequence or structural similarity. Source: UniProtKB cadmium ion homeostasis Inferred from sequence or structural similarity. Source: UniProtKB cellular lipid catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cellular response to cadmium ion Inferred from sequence or structural similarity. Source: UniProtKB cellular response to drug Inferred from sequence or structural similarity. Source: UniProtKB cellular response to nitric oxide Inferred from sequence or structural similarity. Source: UniProtKB cellular zinc ion homeostasis Inferred from sequence or structural similarity. Source: UniProtKB cholesterol catabolic process Inferred from sequence or structural similarity. Source: UniProtKB energy reserve metabolic process Inferred from sequence or structural similarity. Source: UniProtKB histone modification Inferred from sequence or structural similarity. Source: UniProtKB leptin-mediated signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of autophagy Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of axon extension Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of necrotic cell death Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of neuron apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of oxidoreductase activity Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of reactive oxygen species metabolic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of ERK1 and ERK2 cascade Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of lysosomal membrane permeability Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of necrotic cell death Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of oxygen metabolic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of vascular endothelial growth factor receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB protein import into nucleus, translocation Inferred from sequence or structural similarity. Source: UniProtKB protein kinase B signaling cascade Inferred from sequence or structural similarity. Source: UniProtKB protein stabilization Inferred from sequence or structural similarity. Source: UniProtKB regulation of protein glycosylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of response to food Inferred from sequence or structural similarity. Source: UniProtKB removal of superoxide radicals Inferred from sequence or structural similarity. Source: UniProtKB response to hypoxia Inferred from sequence or structural similarity. Source: UniProtKB zinc ion transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	inclusion body Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB synaptic vesicle Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cadmium ion binding Inferred from sequence or structural similarity. Source: UniProtKB copper ion binding Inferred from sequence or structural similarity. Source: UniProtKB cysteine-type endopeptidase inhibitor activity involved in apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB drug binding Inferred from sequence or structural similarity. Source: UniProtKB protein kinase activator activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 68	68	Metallothionein-3		PRO_0000197249
Regions
	Region	1 – 30	30	Beta
	Region	31 – 68	38	Alpha
Sites
	Metal binding	6	1	Divalent metal cation; cluster B By similarity
	Metal binding	8	1	Divalent metal cation; cluster B By similarity
	Metal binding	14	1	Divalent metal cation; cluster B By similarity
	Metal binding	16	1	Divalent metal cation; cluster B By similarity
	Metal binding	20	1	Divalent metal cation; cluster B By similarity
	Metal binding	22	1	Divalent metal cation; cluster B By similarity
	Metal binding	25	1	Divalent metal cation; cluster B By similarity
	Metal binding	27	1	Divalent metal cation; cluster B By similarity
	Metal binding	30	1	Divalent metal cation; cluster B By similarity
	Metal binding	34	1	Divalent metal cation; cluster A By similarity
	Metal binding	35	1	Divalent metal cation; cluster A By similarity
	Metal binding	37	1	Divalent metal cation; cluster A By similarity
	Metal binding	38	1	Divalent metal cation; cluster A By similarity
	Metal binding	42	1	Divalent metal cation; cluster A By similarity
	Metal binding	45	1	Divalent metal cation; cluster A By similarity
	Metal binding	49	1	Divalent metal cation; cluster A By similarity
	Metal binding	51	1	Divalent metal cation; cluster A By similarity
	Metal binding	64	1	Divalent metal cation; cluster A By similarity
	Metal binding	66	1	Divalent metal cation; cluster A By similarity
	Metal binding	67	1	Divalent metal cation; cluster A By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine

Sequences

	Sequence		Length	Mass (Da)
	P37360 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 5998E0F17FC52CE6	FASTA	68	6,927
	10 20 30 40 50 60 MDPETCPCPT GGSCTCSGEC KCEGCKCTSC KKSCCSCCPA ECEKCAKDCV CKGGEGAEAE AEKCSCCQ

References

[1]

"Isolation, primary structures and metal binding properties of neuronal growth inhibitory factor (GIF) from bovine and equine brain." Pountney D.L., Fundel S.M., Faller P., Birchler N.E., Hunziker P., Vasak M. FEBS Lett. 345:193-197(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Brain.

Cross-references

Sequence databases
PIR	S44392.
3D structure databases
ProteinModelPortal	P37360.
SMR	P37360. Positions 32-68.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOGENOM	HOG000236262.
InParanoid	P37360.
Family and domain databases
Gene3D	4.10.10.10. 1 hit.
InterPro	IPR017854. Metalthion_dom. IPR023587. Metalthion_dom_vert. IPR003019. Metalthion_sfam_euk. IPR000006. Metalthion_vert. IPR018064. Metalthion_vert_metal_BS. [Graphical view]
PANTHER	PTHR23299. PTHR23299. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
SUPFAM	SSF57868. Metallothionein_sfam. 1 hit.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MT3_HORSE

Accession

Primary (citable) accession number: P37360

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program