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Protein

Metallothionein-3

Gene

MT3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds heavy metals. Contains five zinc and one copper atoms per polypeptide chain and only a negligible amount of cadmium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi6 – 61Divalent metal cation; cluster BBy similarity
Metal bindingi8 – 81Divalent metal cation; cluster BBy similarity
Metal bindingi14 – 141Divalent metal cation; cluster BBy similarity
Metal bindingi16 – 161Divalent metal cation; cluster BBy similarity
Metal bindingi20 – 201Divalent metal cation; cluster BBy similarity
Metal bindingi22 – 221Divalent metal cation; cluster BBy similarity
Metal bindingi25 – 251Divalent metal cation; cluster BBy similarity
Metal bindingi27 – 271Divalent metal cation; cluster BBy similarity
Metal bindingi34 – 341Divalent metal cation; cluster ABy similarity
Metal bindingi35 – 351Divalent metal cation; cluster ABy similarity
Metal bindingi37 – 371Divalent metal cation; cluster ABy similarity
Metal bindingi38 – 381Divalent metal cation; cluster ABy similarity
Metal bindingi42 – 421Divalent metal cation; cluster ABy similarity
Metal bindingi45 – 451Divalent metal cation; cluster ABy similarity
Metal bindingi49 – 491Divalent metal cation; cluster ABy similarity
Metal bindingi51 – 511Divalent metal cation; cluster ABy similarity
Metal bindingi64 – 641Divalent metal cation; cluster ABy similarity
Metal bindingi66 – 661Divalent metal cation; cluster ABy similarity
Metal bindingi67 – 671Divalent metal cation; cluster ABy similarity

GO - Molecular functioni

  1. cadmium ion binding Source: UniProtKB
  2. copper ion binding Source: UniProtKB
  3. cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: UniProtKB
  4. drug binding Source: UniProtKB
  5. protein kinase activator activity Source: UniProtKB
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. activation of protein kinase B activity Source: UniProtKB
  2. astrocyte development Source: UniProtKB
  3. cadmium ion homeostasis Source: UniProtKB
  4. cellular lipid catabolic process Source: UniProtKB
  5. cellular response to cadmium ion Source: UniProtKB
  6. cellular response to drug Source: UniProtKB
  7. cellular response to hypoxia Source: Ensembl
  8. cellular response to nitric oxide Source: UniProtKB
  9. cellular response to oxidative stress Source: UniProtKB
  10. cellular zinc ion homeostasis Source: UniProtKB
  11. cholesterol catabolic process Source: UniProtKB
  12. energy reserve metabolic process Source: UniProtKB
  13. ERK1 and ERK2 cascade Source: UniProtKB
  14. histone modification Source: UniProtKB
  15. leptin-mediated signaling pathway Source: UniProtKB
  16. negative regulation of apoptotic process Source: UniProtKB
  17. negative regulation of autophagy Source: UniProtKB
  18. negative regulation of axon extension Source: UniProtKB
  19. negative regulation of cell growth Source: UniProtKB
  20. negative regulation of cysteine-type endopeptidase activity Source: UniProtKB
  21. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  22. negative regulation of necrotic cell death Source: UniProtKB
  23. negative regulation of neuron apoptotic process Source: UniProtKB
  24. negative regulation of oxidoreductase activity Source: UniProtKB
  25. negative regulation of reactive oxygen species metabolic process Source: UniProtKB
  26. negative regulation of transcription, DNA-templated Source: UniProtKB
  27. positive regulation of catalytic activity Source: UniProtKB
  28. positive regulation of cell death Source: UniProtKB
  29. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  30. positive regulation of gene expression Source: UniProtKB
  31. positive regulation of lysosomal membrane permeability Source: UniProtKB
  32. positive regulation of necrotic cell death Source: UniProtKB
  33. positive regulation of oxygen metabolic process Source: UniProtKB
  34. positive regulation of protein phosphorylation Source: UniProtKB
  35. positive regulation of transcription, DNA-templated Source: UniProtKB
  36. positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: UniProtKB
  37. positive regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
  38. protein import into nucleus, translocation Source: UniProtKB
  39. protein kinase B signaling Source: UniProtKB
  40. protein stabilization Source: UniProtKB
  41. regulation of protein glycosylation Source: UniProtKB
  42. regulation of response to food Source: UniProtKB
  43. removal of superoxide radicals Source: UniProtKB
  44. response to hypoxia Source: UniProtKB
  45. zinc ion homeostasis Source: UniProtKB
  46. zinc ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Copper, Metal-binding, Metal-thiolate cluster, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-3
Short name:
MT-3
Alternative name(s):
Growth inhibitory factor
Short name:
GIF
Metallothionein-III
Short name:
MT-III
Gene namesi
Name:MT3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 18

Subcellular locationi

GO - Cellular componenti

  1. inclusion body Source: UniProtKB
  2. intracellular Source: UniProtKB
  3. nucleus Source: UniProtKB
  4. perinuclear region of cytoplasm Source: UniProtKB
  5. synaptic vesicle Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6868Metallothionein-3PRO_0000197248Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine

Keywords - PTMi

Acetylation

Expressioni

Tissue specificityi

Brain.

Structurei

3D structure databases

ProteinModelPortaliP37359.
SMRiP37359. Positions 32-68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3030BetaAdd
BLAST
Regioni31 – 6838AlphaAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00730000111484.
HOGENOMiHOG000236262.
HOVERGENiHBG095156.
InParanoidiP37359.
KOiK14740.
OMAiVCKGGEG.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.

Sequencei

Sequence statusi: Complete.

P37359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPETCPCPT GGSCTCSDPC KCEGCTCASS KKSCCSCCPA ECEKCAKDCV
60
CKGGEGAEAE EKKCSCCQ
Length:68
Mass (Da):6,937
Last modified:April 17, 2007 - v2
Checksum:i1DD24B83AA52AE71
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301S → C AA sequence (PubMed:8200454).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126773 mRNA. Translation: AAI26774.1.
PIRiS44391.
RefSeqiNP_001106775.1. NM_001113304.2.
UniGeneiBt.16213.

Genome annotation databases

EnsembliENSBTAT00000022460; ENSBTAP00000022460; ENSBTAG00000016886.
GeneIDi613320.
KEGGibta:613320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126773 mRNA. Translation: AAI26774.1.
PIRiS44391.
RefSeqiNP_001106775.1. NM_001113304.2.
UniGeneiBt.16213.

3D structure databases

ProteinModelPortaliP37359.
SMRiP37359. Positions 32-68.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000022460; ENSBTAP00000022460; ENSBTAG00000016886.
GeneIDi613320.
KEGGibta:613320.

Organism-specific databases

CTDi4504.

Phylogenomic databases

GeneTreeiENSGT00730000111484.
HOGENOMiHOG000236262.
HOVERGENiHBG095156.
InParanoidiP37359.
KOiK14740.
OMAiVCKGGEG.

Miscellaneous databases

NextBioi20898520.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, primary structures and metal binding properties of neuronal growth inhibitory factor (GIF) from bovine and equine brain."
    Pountney D.L., Fundel S.M., Faller P., Birchler N.E., Hunziker P., Vasak M.
    FEBS Lett. 345:193-197(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Brain.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal pons.

Entry informationi

Entry nameiMT3_BOVIN
AccessioniPrimary (citable) accession number: P37359
Secondary accession number(s): A0JNL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 17, 2007
Last modified: January 7, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.