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Protein

Ribose-5-phosphate isomerase B

Gene

rpiB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of ribulose-5-P and ribose-5-P. It probably also has activity on D-allose 6-phosphate.4 Publications

Catalytic activityi

D-ribose 5-phosphate = D-ribulose 5-phosphate.2 Publications
D-allose 6-phosphate = D-allulose 6-phosphate.1 Publication

Enzyme regulationi

Inhibited by iodoacetate and glucose 6-phosphate.2 Publications

Kineticsi

  1. KM=0.83 mM for D-ribose 5-phosphate (at 37 degrees Celsius)1 Publication
  2. KM=1.23 mM for D-ribose 5-phosphate1 Publication
  3. KM=0.5 mM for D-allose 6-phosphate1 Publication

    Temperature dependencei

    After incubation at 45 degrees Celsius for 30 minutes RpiB retains 65% of its original activities. At 60 degrees Celsius RpiB is rapidly inactivated.3 Publications

    Pathwayi: pentose phosphate pathway

    This protein is involved in step 1 of the subpathway that synthesizes D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage).
    Proteins known to be involved in this subpathway in this organism are:
    1. Ribose-5-phosphate isomerase B (rpiB), Ribose-5-phosphate isomerase A (rpiA)
    This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei66Proton acceptor1 Publication1
    Active sitei99Proton donor1 Publication1
    Binding sitei100D-ribulose 5-phosphateBy similarity1
    Binding sitei110D-ribulose 5-phosphateBy similarity1
    Binding sitei133D-ribulose 5-phosphateBy similarity1
    Binding sitei137D-ribulose 5-phosphateBy similarity1

    GO - Molecular functioni

    • allose 6-phosphate isomerase activity Source: EcoCyc
    • ribose-5-phosphate isomerase activity Source: EcoCyc

    GO - Biological processi

    • D-allose catabolic process Source: EcoCyc
    • pentose-phosphate shunt Source: UniProtKB-UniPathway

    Keywordsi

    Molecular functionIsomerase
    Biological processCarbohydrate metabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:RIB5PISOMB-MONOMER
    MetaCyc:RIB5PISOMB-MONOMER
    SABIO-RKP37351
    UniPathwayiUPA00115; UER00412

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribose-5-phosphate isomerase B1 Publication (EC:5.3.1.62 Publications)
    Alternative name(s):
    Phosphoriboisomerase B1 Publication
    Gene namesi
    Name:rpiB1 Publication
    Synonyms:yjcA
    Ordered Locus Names:b4090, JW4051
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11827 rpiB

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi99H → N: Strongly reduced enzyme activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002081631 – 149Ribose-5-phosphate isomerase BAdd BLAST149

    Proteomic databases

    PaxDbiP37351
    PRIDEiP37351

    Expressioni

    Inductioni

    Induced by ribose and repressed by RpiR.2 Publications

    Interactioni

    Subunit structurei

    Homodimer, and homotetramer.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    rplJP0A7J32EBI-557460,EBI-546827

    Protein-protein interaction databases

    BioGridi4262683, 7 interactors
    DIPiDIP-10740N
    IntActiP37351, 3 interactors
    STRINGi316385.ECDH10B_4281

    Structurei

    Secondary structure

    1149
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 8Combined sources6
    Helixi12 – 15Combined sources4
    Helixi16 – 25Combined sources10
    Beta strandi29 – 32Combined sources4
    Beta strandi37 – 39Combined sources3
    Helixi43 – 55Combined sources13
    Beta strandi58 – 69Combined sources12
    Helixi70 – 77Combined sources8
    Beta strandi84 – 86Combined sources3
    Helixi90 – 100Combined sources11
    Beta strandi104 – 108Combined sources5
    Turni109 – 111Combined sources3
    Helixi114 – 126Combined sources13
    Helixi134 – 145Combined sources12

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1NN4X-ray2.20A/B/C/D1-149[»]
    2VVRX-ray2.10A/B/C/D/E/F1-149[»]
    ProteinModelPortaliP37351
    SMRiP37351
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP37351

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni9 – 10D-ribulose 5-phosphate bindingBy similarity2
    Regioni67 – 71D-ribulose 5-phosphate bindingBy similarity5

    Sequence similaritiesi

    Belongs to the LacAB/RpiB family.Curated

    Phylogenomic databases

    eggNOGiENOG4108YZG Bacteria
    COG0698 LUCA
    HOGENOMiHOG000226301
    InParanoidiP37351
    KOiK01808
    OMAiDDYPPFC
    PhylomeDBiP37351

    Family and domain databases

    Gene3Di3.40.1400.10, 1 hit
    InterProiView protein in InterPro
    IPR004785 RpiB
    IPR003500 RpiB_LacA_LacB
    IPR036569 RpiB_LacA_LacB_sf
    PANTHERiPTHR30345 PTHR30345, 1 hit
    PfamiView protein in Pfam
    PF02502 LacAB_rpiB, 1 hit
    PIRSFiPIRSF005384 RpiB_LacA_B, 1 hit
    SUPFAMiSSF89623 SSF89623, 1 hit
    TIGRFAMsiTIGR01120 rpiB, 1 hit
    TIGR00689 rpiB_lacA_lacB, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P37351-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKKIAFGCDH VGFILKHEIV AHLVERGVEV IDKGTWSSER TDYPHYASQV
    60 70 80 90 100
    ALAVAGGEVD GGILICGTGV GISIAANKFA GIRAVVCSEP YSAQLSRQHN
    110 120 130 140
    DTNVLAFGSR VVGLELAKMI VDAWLGAQYE GGRHQQRVEA ITAIEQRRN
    Length:149
    Mass (Da):16,073
    Last modified:February 1, 1995 - v2
    Checksum:iBE610B5F995CEEB6
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X82203 Genomic DNA Translation: CAA57688.1
    U14003 Genomic DNA Translation: AAA96989.1
    U00096 Genomic DNA Translation: AAC77051.1
    AP009048 Genomic DNA Translation: BAE78093.1
    D90227 Genomic DNA Translation: BAA21501.1
    PIRiJC6054
    RefSeqiNP_418514.1, NC_000913.3
    WP_000716794.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC77051; AAC77051; b4090
    BAE78093; BAE78093; BAE78093
    GeneIDi948602
    KEGGiecj:JW4051
    eco:b4090
    PATRICifig|1411691.4.peg.2610

    Entry informationi

    Entry nameiRPIB_ECOLI
    AccessioniPrimary (citable) accession number: P37351
    Secondary accession number(s): O32564, Q2M6L3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: February 1, 1995
    Last modified: March 28, 2018
    This is version 145 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

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